TRMT2B (NM_024917) Human Untagged Clone

CAT#: SC108366

TRMT2B (untagged)-Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRMT2B
• Synonyms: CXorf34; dJ341D10.3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024917, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGGCCTTAAGAGAAGAGTCCCACTGCACAGCCTCAGATACTTCATCTCCATGGTGGGTCTCTTCT
  CCAAACCAGGACTGCTTCCCTGGTATGCCAGAAATCCACCAGGATGGTCACAGCTCTTTCTGGGCACAGT
  ATGTAAGGGAGATTTCACCCGTGTGATAGCCACGAAATGTCAGAAAGGACAAAAAAGTCAGAAGAAACCA
  AGCCATCTTGGACCACTAGATGGTTCCTGGCAGGAAAGGCTGGCTGATGTTGTGACACCACTCTGGAGGT
  TGAGCTATGAAGAACAGCTCAAGGTGAAATTTGCAGCTCAGAAGAAAATTTTACAAAGACTAGAGTCTTA
  CATCCAAATGCTCAATGGAGTCAGTGTGACAACGGCTGTACCCAAATCTGAGAGGCTCTCTTGTCTTCTC
  CATCCTATTATACCCTCTCCTGTCATCAATGGTTACCGAAATAAGTCCACCTTCTCTGTGAACCGAGGTC
  CAGATGGCAATCCAAAGACTGTGGGGTTCTACCTGGGAACTTGGAGAGATGGGAACGTTGTCTGTGTGCA
  GTCTAATCATCTGAAAAACATCCCTGAGAAACACAGTCAAGTGGCGCAGTACTATGAAGTATTCCTTCGA
  CAGTCTCCATTGGAGCCCTGCCTTGTATTTCATGAAGGTGGATACTGGCGTGAGCTCACAGTCCGCACCA
  ATAGCCAAGGGCACACAATGGCTATCATCACTTTCCATCCCCAGAAATTAAGTCAGGAGGAGCTCCATGT
  TCAGAAGGAGATTGTAAAGGAATTTTTCATCAGAGGTCCTGGAGCAGCCTGTGGCTTGACCTCACTTTAC
  TTCCAGGAAAGTACCATGACCCGTTGCAGCCATCAGCAGTCTCCCTATCAGCTTCTGTTTGGGGAACCCT
  ACATCTTTGAAGAACTTCTGAGCTTGAAGATCCGCATCTCTCCAGATGCCTTTTTCCAGATTAACACAGC
  TGGTGCAGAGATGCTGTATCGGACTGTGGGGGAGCTGACTGGAGTGAACTCTGACACCATCCTTCTTGAC
  ATCTGCTGTGGAACTGGTGTGATTGGCCTCTCTCTGGCTCAGCATACATCTCGGGTCCTTGGGATTGAAT
  TGTTGGAGCAGGCAGTGGAGGATGCAAGATGGACTGCAGCCTTCAATGGCATCACCAACTCTGAATTTCA
  TACTGGTCAAGCAGAGAAGATTTTGCCAGGGCTGCTAAAGTCAAAGGAAGATGGACAGTCAATTGTTGCT
  GTGGTGAACCCAGCCCGTGCCGGACTGCATTACAAGGTGATTCAAGCCATTCGAAACTTCAGGGCCATCC
  ACACGCTAGTTTTTGTTTCCTGCAAGCTCCATGGTGAATCCACTAGGAATGTCATTGAGCTGTGCTGTCC
  TCCAGACCCTGCTAAGAAGCTCTTAGGCGAGCCCTTTGTCCTGCAGCAAGCTGTACCTGTGGATTTGTTC
  CCTCACACCCCACATTGTGAGCTGGTGCTCCTCTTTACTCGATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024917 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTATGAGTGCTTTT
  AAGGAAGGTCTCCATAGAAGGTTCCCCCAGCAAAGTAGGGAGGGGTACTCCCCGAGAGGA
  GTCGAGGTGCCGGAGCCTGCTGCCTCGCCGACGCTGGGAGGAGCCCCGTGTCCCGCCGCG
  CTTGCCAAGGGTTGGCTGCGCGTGCGGCGGGAGTAGAGGCGCCTTGCGCACCAGGAAGTG
  ACTGTTTCCCCGCCGCAGCAAACCAGGCCATCCGCTGGCCTTTTAGTTTGCCGCCTCAGG
  TGAAATTTGCAGCTCAGAAGAAAATTTTACAAAGACTAGAGTCTTACATCCAAATGCTCA
  ATGGAGTCAGTGTGACAACGGCTGTACCCAAATCTGAGAGGCTCTCTTGTCTTCTCCATC
  CTATTATACCCTCTCCTGTCATCAATGGTTACCGAAATAAGTCCACCTTCTCTGTGAACC
  GAGGTCCAGATGGCAATCCAAAGACTGTGGGGTTCTACCTGNGAACTTGGAGAGATGGGA
  ACGTTGTCTGTGTGCAGTCTAATCATCTGAAAAACATCCCTGAGAAACACAGTCAAGTGG
  CGCAGACGGAGTTTTGCTCTGTCACCCAGGCTGGAGTGCTGTGGTGTGATCTCNGCTCGC
  TGCAACTTCTGCCTCCTGCGTTCCAGCGATTCTCCTGCTCAGCCCCCTGAGTACCTGGGA
  TTGCAGGTGGATACTGCCGTGAGCTCCCAGTCCGCACCATAGCCAGGCCCCACAATGGCT
  ATCATCACTTTCCATTCCCAGAAATTAAGTTAGGAGGAGCCTCCTTGTTCAGAAGGAGAT
  TGCAAAAGAATTCTTCATTCAAAGGCCCTGAAACAGCCCTGTGCCTTGACCTCACTTTAC
  TTCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024917 unedited
  NTTCTTTGGAACCGCGGCCGCATTTCTANGATCGAGTTTTTTTTTTTTTTTTTTGAGAGG
  TGTCTCACTCTGTCACCCAGGCTGGAGTGCAGCGGCACAATCCCAGCCCACTGCAACCTC
  CACCTCCCAGGCCCAAGCAATTATCCTGCCTCAGCCTCCCGAGTAGCCGGGATTACAGGT
  GCCCACCACCAGGGGAAAACTACATTTTCAAGGGGCATTCGAGATGCCTTAAAACCAAGA
  AAACCCAAAAGAATTAATCTCATCTGATGATCACTCAGTCACCCTGTATTCACTTCATGG
  CTTACCCTGCTTTTAAAAAACCTCGACTGTTTCTGGTTTTAATCCTTAACTACCTCAAGA
  ATCCAGAAGTCTTAGAAATATCTAAAAATTGTAGCATCTCTGAGCAAATGCACAGGCTCC
  AGTCAATGTAAAATTATTACCAGCTGCTTAAAAAAAGGTCATTTGTCCACACCATAAGCA
  GAAACGGAGGAGACAAGACTATAAACTAGAACCAAGGCTGACAGCAACAGGAAGAAACAG
  TCCAGATCCAGGAGTGGGGAGTGGTCAAACCCAAGCAAACGGGCATGATTGAAAGGGAAT
  CTGAAATCTAAGGTTAAGTCACCCGCGGCGGCGTGTGGGTGGCTCACGCCTATAATCCCA
  TCACTTTGAGAGGCCCACGCCGGGTGCATCACCTTGAGGCCACGAGTTCGAGACCAGCCT
  GGCCAACAGGGGGAAACCCTGCTTTACCAAAAATACAAACTTATCCCGGGCTGGGGCACT
  CCCCTTTAATCCCAATCCCGGCGAAACCGAGCACCAAAATACCCCTATCCGGGAGGCGGA
  AGCCGTTCGAGCCTATAACCTCCCCGTACTTCCACCCGGGCAATATGTAACCCCTTCCAA
  AAAAACAAGTGTCTTGAACACAGGCCTAAGAATTTCTCTTTAAAACCAAGATGGCCCCCC
  GT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024917
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024917.3, NP_079193.2
• RefSeq Size: 3574 bp
• RefSeq ORF: 1515 bp
• Locus ID: 79979
• UniProt ID: Q96GJ1
• Cytogenetics: Xq22.1
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 4 all encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.