MIR16 (GDE1) (NM_016641) Human Untagged Clone

CAT#: SC108185

GDE1 (untagged)-Human glycerophosphodiester phosphodiesterase 1 (GDE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MIR16
• Synonyms: 363E6.2; MIR16
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016641, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCTGTGGGAGGACCAGGGCGGCCTCCTGGGCCCTTTCTCCTTCCTGCTGCTAGTGCTGCTGCTGG
  TGACGCGGAGCCCGGTCAATGCCTGCCTCCTCACCGGCAGCCTCTTCGTTCTACTGCGCGTCTTCAGCTT
  TGAGCCGGTGCCCTCTTGCAGGGCCCTGCAGGTGCTCAAGCCCCGGGACCGCATTTCTGCCATCGCCCAC
  CGTGGCGGCAGCCACGACGCGCCCGAGAACACGCTGGCGGCCATTCGGCAGGCAGCTAAGAATGGAGCAA
  CAGGCGTGGAGTTGGACATTGAGTTTACTTCTGACGGGATTCCTGTCTTAATGCACGATAACACAGTAGA
  TAGGACGACTGATGGGACTGGGCGATTGTGTGATTTGACATTTGAACAAATTAGGAAGCTGAATCCTGCA
  GCAAACCACAGACTCAGGAATGATTTCCCTGATGAAAAGATCCCTACCCTAAGGGAAGCTGTTGCAGAGT
  GCCTAAACCATAACCTCACAATCTTCTTTGATGTCAAAGGCCATGCACACAAGGCTACTGAGGCTCTAAA
  GAAAATGTATATGGAATTTCCTCAACTGTATAATAATAGTGTGGTCTGTTCTTTCTTGCCAGAAGTTATC
  TACAAGATGAGACAAACAGATCGGGATGTAATAACAGCATTAACTCACAGACCTTGGAGCCTAAGCCATA
  CAGGAGATGGGAAACCACGCTATGATACTTTCTGGAAACATTTTATATTTGTTATGATGGACATTTTGCT
  CGATTGGAGCATGCATAATATCTTGTGGTACCTGTGTGGAATTTCAGCTTTCCTCATGCAAAAGGATTTT
  GTATCCCCGGCCTACTTGAAGAAGTGGTCAGCTAAAGGAATCCAGGTTGTTGGTTGGACTGTTAATACCT
  TTGATGAAAAGAGTTACTACGAATCCCATCTTGGTTCCAGCTATATCACTGACAGCATGGTAGAAGACTG
  CGAACCTCACTTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016641 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCATTGTGCGGCGCTGG
  TCCCCTCAGAGGGTTCCTGCTGCTGCCGGTGCCTTGGACCCTCCCCCTCGCTTCTCGTTC
  TACTGCCCCAGGAGCCCGGCGGGTCCGGGACTCCCGTCCGTGCCGGTGCGGGCGCCGGCA
  TGTGGCTGTGGGAGGACCAGGGCGGCCTCCTGGGCCCTTTCTCCTTCCTGCTGCTAGTGC
  TGCTGCTGGTGACGCGGAGCCCGGTCAATGCCTGCCTCCTCACCGGCAGCCTCTTCGTTC
  TACTGCGCGTCTTCAGCTTTGAGCCGGTGCCCTCTTGCAGGGCCCTGCAGGTGCTCAAGC
  CCCGGGACCGCATTTCTGCCATCGCCCACCGTGGCGGCAGCCACGACGCGCCCGAGAACA
  CGCTGGCGGCCATTCGGCAGGCAGCTAAGAATGGAGCAACAGGCGTGGAGTTGGACATTG
  AGTTTACTTCTGACGGGATTCCTGTCTTAATGCACGATAACACAGTAGATAGGACGACTG
  ATGGGACTGGGCGATTGTGTGATTTGACATTTGAACAAATTAGGAAGCTGAATCCTGCAG
  CAAACCACAGACTCAGGAATGATTTCCCTGATGAAAAGATCCCTACCCTANAGGAAGCTG
  TTGCAGAGTGCCTAAACCATAACCTCACAATCTTCTTTGATGTCAAAGGCCATGCACACA
  AGGCTACTGAGGCTCTAAGANAATGTATATGGAATTTCNCTCACTGTATATATANNTGTG
  GTCTGGTCTTTCTTTGCCAGAGTTATCTANCAGATGGACNAACAGATCCGGATGGTATAC
  AGCATTACTCACAGACCTTGAGCCTAGCCTACAGAGAGGGNAACACCTTTGAACTTCTGG
  ACATNTATATNGNATGAGGGCATTGCTCATGGACTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016641 unedited
  CCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAGATCAGGTTCTCACT
  GTGCCACCAAGGTTGTAGTGCCGTGGTGTAATCATGGCTCATTGCAGCCTTGAACTTCTG
  GGCTCAAGTGATCCTCCCACCTCAACCTCCTGAGTAGCTGGGAATACAGGCATGCACCAC
  CATGCCTAATTTTTTAGAGATGGGGTCTTGCTATATTGCCCAGTCTGGCCTCAAGTAATC
  CTCCTGCCTCAGCTTTCTGAATTGCTGGGATTATCGGCATGAGCCACTGTGCCCAGCTAA
  GCTGTGACTTTTGAGGCAACCCCTTCCCTTCCACAGAGTTTAGTTTCCTCATTTGTAAAA
  TGAGGACAAACCTAGATATTTTTGGAAGTTTCTTCCAGTTCTAATTGCCTATGATCAGAT
  CACATCTGTTGCAGAGCTTCCTAAAAAGCCTGGAAAAGTGCAACTCTGCCCCCTGGTGGT
  TTGCAGCTCTCTATTCAAGATGCTATAAATCCCTCATTGGCTTCAAGACCTCCCTACCAT
  TTTGGTTGCTGTACACTGAAGCCTCACTGGATTGCATGGTGGCTTCCCCAAATTCCGTAT
  GCCTTANGATATGATGCTTGGCACACAGTTTTCCTTTAGTGCTCCTTTTAATAATTTTAT
  TAGTATGGCCACAAGTTTGATGTCTACAGTACATGTTAACATAGCTGAGTACAAATATTT
  GAAATAAGTGTGGCAAGCTTTAAAATGCAACTTTTGAGTTATCATGCCTGTCCCATGCCT
  TTACCTTTGGCTCTGCAAATTGTCCAATTCAATCTGGGCTTATCCTCACTGGGTCTCCCT
  GGGGCCCTAGCTAAGCAGGGCAAGGGCTCTCGGGCGTTNTTTAAGACCAAATTTTCAAAG
  ACACACGGTTGAAATCTGGCTGCCCATGGGCATGGGGGAACCCCGGGTTATCTTTGGTCA
  GAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016641
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016641.3, NP_057725.1
• RefSeq Size: 2960 bp
• RefSeq ORF: 996 bp
• Locus ID: 51573
• UniProt ID: Q9NZC3
• Cytogenetics: 16p12.3
• Domains: GDPD
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: Has glycerophosphoinositol phosphodiesterase activity. Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function]