ZNF289 (ARFGAP2) (NM_032389) Human Untagged Clone

CAT#: SC104758

ARFGAP2 (untagged)-Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARFGAP2
• Synonyms: IRZ; NBLA10535; ZFP289; ZNF289
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032389, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGAGCCGAACAAGACCGAAATCCAGACTCTTTTTAAGAGGCTTCGCGCAGTTCCAACCAACA
  AGGCCTGTTTCGACTGCGGCGCCAAGAATCCGAGTTGGGCCAGCATCACGTACGGTGTTTTCTTGTGCAT
  TGACTGTTCCGGGGTGCACCGCTCCCTGGGCGTCCATCTGAGCTTCATCAGGTCCACAGAGTTGGATTCC
  AACTGGAACTGGTTCCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACGGCTTTTTTTCGCC
  AACATGGATGCACAGCCAATGATGCCAACACCAAATATAATAGCCGAGCTGCCCAGATGTACCGGGAGAA
  GATCCGGCAGCTGGGGAGTGCGGCCCTGGCTAGGCATGGCACTGATCTTTGGATAGACAACATGAGTAGT
  GCCGTTCCTAATCACTCCCCAGAGAAGAAGGACTCTGATTTCTTCACAGAACACACTCAACCCCCTGCCT
  GGGATGCGCCAGCCACTGAGCCTTCAGGGACCCAGCAGCCAGCCCCGTCTACAGAGAGCAGTGGCCTGGC
  ACAGCCGGAGCATGGCCCCAACACAGACCTGCTTGGCACCTCACCCAAAGCCTCACTGGAACTGAAAAGC
  TCCATCATTGGCAAGAAGAAGCCAGCAGCAGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTAGGGGCCC
  AGAAGGTGAGCAGCCAGAGCTTCAGTGAGATTGAGCGGCAGGCTCAGGTGGCAGAGAAGCTCCGTGAGCA
  GCAGGCAGCCGATGCCAAGAAGCAGGCGGAGGAGTCCATGGTCGCCTCCATGCGTCTGGCCTACCAGGAG
  CTCCAGATTGATCGTAAGAAAGAGGAAAAGAAGCTACAGAATCTGGAAGGGAAGAAGCGAGAGCAGGCAG
  AAAGGTTGGGCATGGGCTTGGTATCCCGAAGCTCTGTCTCCCACTCCGTGCTGTCTGAGATGCAGGTGAT
  TGAGCAGGAAACCCCAGTGAGTGCAAAATCCTCTCGCTCGCAGCTGGACTTGTTTGACGATGTTGGTACT
  TTCGCCTCTGGACCCCCAAAGTACAAGGACAATCCCTTTTCCTTAGGGGAAAGCTTTGGCTCCCGCTGGG
  ATACAGATGCTGCCTGGGGTATGGACAGGGTAGAGGAGAAGGAGCCAGAAGTGACCATCTCAAGCATCCG
  GCCTATTTCAGAAAGAGCCACAAACCGGAGGGAAGTGGAGAGCCGGAGCTCAGGCCTCGAGTCTAGTGAG
  GCGCGTCAGAAATTCGCAGGAGCCAAAGCCATCTCATCTGACATGTTCTTTGGGCGGGAGGTGGATGCGG
  AGTATGAGGCCAGGTCTCGGCTGCAGCAGCTCTCAGGCAGCAGTGCCATCAGCTCTTCAGACCTCTTTGG
  GGACATGGATGGAGCTCACGGAGCAGGAAGTGTATCTCTGGGGAACGTGCTGCCTACAGCGGACATTGCC
  CAGTTTAAGCAGGGTGTCAAGTCTGTGGCTGGGAAAATGGCTGTGCTGGCCAATGGTGTGATGAATTCCT
  TGCAGGATCGCTACGGTTCCTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032389 unedited
  TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAAGGAGAGAAATGGC
  GGCGGAGCCGAACAAGACCGAAATCCAGACTCTTTTTAAGAGGCTTCGCGCAGTTCCAAC
  CAACAAGGCCTGTTTCGACTGCGGCGCCAAGAATCCGAGTTGGGCCAGCATCACGTACGG
  TGTTTTCTTGTGCATTGACTGTTCCGGGGTGCACCGCTCCCTGGGCGTCCATCTGAGCTT
  CATCAGGTCCACAGAGTTGGATTCCAACTGGAACTGGTTCCAGCTGAGGTGTATGCAGGT
  CGGCGGGAATGCCAATGCGACGGCTTTTTTTCGCCAACATGGATGCACAGCCAATGATGC
  CAACACCAAATATAATAGCCGAGCTGCCCAGATGTACCGGGAGAAGATCCGGCAGCTGGG
  GAGTGCGGCCCTGGCTAGGCATGGCACTGATCTTTGGATAGACAACATGAGTAGTGCCGT
  TCCTAATCACTCCCCAGAGAAGAAGGACTCTGATTTCTTCACAGAACACACTCAACCCCC
  TGCCTGGGATGCGCCAGCCACTGAGCCTTCAGGGACCCAGCAGCCAGCCCCGTCTACAGA
  GAGCAGTGGCCTGGCACAGCCGGAGCATGGCCCCAACACAGACCTGCTTGGCACCTCACC
  CAAAGCCTCACTGGAGTCCGTGTATCTGTCAGCTAAAGGGGCCCTTCCTGCCAGAGAACT
  GAAAAGCTCCATCATTGGCAAGAAGAAGCCAGCAGCAGCTAAGAAAGGGCTGGGTGCCAA
  AANAGGGCCTAGGGCCCCAAAAGTGAGCAGCCAGAGCTTCAGTGAGATTGAGCGGCAGCT
  CAGGTGCAAAAAGCCTCCTGAGCGNNCAGCACCGATGCCAAGAGCAGCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032389 unedited
  CTTGGACCGCGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCACTCAAACCAAC
  AGGGANAGCCAGGCCCTGCNAGGGGTTTTATTTTGACACCACTTTGTTTCAATACAAACA
  GTCCAGAAAGAAAGTCAAGTCCCTCTGGGGGAGGGGCAAGGGGAAGAGTGGTCTGTGTTG
  CTTGGGAGCCCAACCTACAACCCAAAGGTGGGGGCTGGGCTGAAACTGCCGGTGCGGCAG
  GGGAAGATGGCACCAAGAATGACAGTGCTTGGCTCAGCTGCCAGAGGGTGAGGCCCACAG
  CTCTCACTGGCGGGTGCTGTCCAGGCCAAGCCCAGAATGATGCAGAGGAAGGAGCTCAGC
  CCCAGGAGCCTGCCTCTGCCTCTCACATCCTCTGCTTCCCTGGCCAGCATCAAGCTCACA
  GCATCCAGAGTCGAATCACAGCAGACAAGACCCTCCATGGCCACCAACCCGGGGAAGAAG
  GGATAAAGAATGTCACAGAGGGTCCTGCATTCCACAGCAGATGGTACAGAGGGACCCTGA
  GACACCCTGAAACAATCTTCCAAGTGCTTACTGTAAAAAGGGGTGGTGAGACGGGAGAAC
  CCAAGGGTCTCCGGTCTGATACCAAATGAAACCTTTAGGTACCAGGTAAAGTTTCCAGCC
  CTTGGTAACGCCCAGACAAGGTCCTTCCCCTCTCCTGGCACCAGAAGAGGCCACTGGCAG
  CAGCAGCAGCAGCAAGTCCGCAGCCTCTTTCCCCATGGACGGGTCTACAGTGAACTCCTT
  CCANNGCCTCCCCTGNTTCCAGAGATGGGGAACCCAAGCCCTCAACTAAAGAACCCTANA
  GCANCANANAAGCTTCTACTTNCGCTTTCCTACCTGGGAAAGNCTGNACCANAATAGCCA
  TCTGGGGGACCCCTTTCCATATGCCAGAAATGACACCACTGTGGGGCAGACAAAGACAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032389
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032389.3, NP_115765.2
• RefSeq Size: 3039 bp
• RefSeq ORF: 3039 bp
• Locus ID: 84364
• UniProt ID: Q8N6H7
• Cytogenetics: 11p11.2
• Domains: ArfGap
• Protein Pathways: Endocytosis
• Gene Summary: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).