NUAK2 (AK056725) Human Untagged Clone

CAT#: SC104381

(untagged)-Human cDNA FLJ32163 fis, clone PLACE6000371

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUAK2
• Synonyms: ACVRLK1; ALK-1; ALK1; HHT; HHT2; ORW2; SKR3; TSR-I
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK056725, the custom clone sequence may differ by one or more nucleotides
  
  
  ATTTGTATCTCAGCTCCATGATGCCTTGGGCTTTCTGTCTCCTCAACAAGAGCGCAGCTTGCTGAATGTC
  AGCTGCCTGAGAGAGCTGGGGCCTGACTTACTAGGGCATTAAATCCTAAGAGGTCCTACTGAGGTGTGGC
  AGGATCACAGGCCAGTGGAAAAAGGGCAGGTCAGATGGGCAAGGCCCAGGACTTTCAGATTAACTGAGAG
  GATATCGAGGCCAAGCATGGCAGGGGGAAGGTCAGTGGGTGTCAAGAGACCCAGGTCTGACCCCGGATGT
  TTGCTCCATGTGACAAAAGCAGGCCTGTCTCAGGACCTTTTCTTTTCTTTTTTCCTTCTTTTTTTTTTTG
  ACACGGAGTTTTGCTCTTGCTGTCCAGGCTAGAGTGCAATGGCATGATCCCAGCTTACCGCAACGTCTAC
  CTCCCAGGTTCAAATCATTCTCTTGCCTCAGACTCCCGAGTAGCTGGGATTACAGGCACATGCCACCATG
  CCTGGCTAATTTTGTATATTTAGTAGAAACAGGGTTTCACCATGTTGGCCATGCTGGTCTCGAACTCCTG
  ACCTCAGGTGTTCCACCTACCTCAGCCTCCCAAAGTGCTGGGGTTACAGGTGTGAGCCATCGCGCCTGGC
  CAGGACCTTTGTTTCTTATCTACATATTGGAAGATTTGGTCCTGATGTCCTTTGAGGCTTCTTTAGCTCT
  AGTTCTCTGACACTTCAGCCTATATCACAGCTAACTTCCTCAGTCTCATCTATTCCTTATGCTCCAGCCC
  CTGGCAATTTGCCTCAAGATGGGGGTTTGAAAATAACTTTACCTGACTCAAGGAGTGTCTGGAGCACCTC
  CTAGTCTAAGTCTGCAAGCTCCAGTTCTTGCCTAAAACCATGCCAGTGGCCACCCTTGGGCTCAGACAGC
  TCTGGGCCTTTTGACCACAAGCCAGCCCCTCGCCCTCTCTGTGGCATAGTCTTCTCTGCCCCAGGACTGC
  AGGGCGGCTTCCTCCAAGGCTTCCAAGGCTCAAAAGAAATTTGGCTCCATCCAAGAAGGCTCCAGCTCCC
  CTACTGGCCCCTGGCTCAGGCCCACACCCCTGGCCAGGCCCAGAGAGTGTGTCTCAGGAGAATTCAATGG
  CTCTAGAGAGACACACAGAAAGTTTGGCATTTGGAAATTTCAAGGATGTATGTATGCTCACGTATGGAGC
  AGGTTGTCCTGGTCCCTGGGTGCAGGGAAGTGGGCTGCAGGGAAGTGGATTGGAGGGGAGCTTGAGGAAT
  ATAAGGAGCGGGGGTGGAGACTCAGGCTATGGACAAGGACAGCCCCAAGGTTGGGAAGACCTGGCCTTAG
  TCGTCCTCAGCCTAGGGGCAGGGCAGTGAAGAAAGCTCTCCCCGCTCCTGCTGTAATGACCCAGAGTAGC
  CTCCCCAGGCCGGCATCTTATGTGTGTCTTCCACCATCCTCATGGTGGCACTTTTCTAGGCCTGTCTCCC
  AGCATTGTGCAAGGCTCGGAAGAGAACCAGGAAGTGAAACTGGGTGAAAACAGAAAGCTCAATGGATGGG
  CTAGGTTCCCAGATCATTAGGGCAGAGTTTGCACGTCCTCTGGTCACTGGAATCCACCCAGCCCACGAAT
  CATCTCCCTCTTGAAGGATTTTATTTCTACTGGGTTTTGGAACAAACTCCTGCTGAGACCCCACAGCCAG
  AAACTGAAAGCAGCAGCTCCCCAAAGCCTGGAAAATCCCTAAGAGAAGGCCTGGGGCAGGAAGTGGAGTG
  ACAGGGGACAGGTAGAGAGAAGGGGGCCCAATGGCCAGGGAGTGAAGGAGGTGGCGTTGCTGAGAGCAGT
  CTGCACATGCTTCTGTCTGAGTGCAGGAAGGTGTTCCAGGGTCGAAATTACACTTCTCGTACCTGGAGAC
  GCTGTTTGTGGGAGCACTGGGCTCATGCCTGGCACACAATAGGTCTGCAATAAACCATGGTTAAATCCTG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK056725 unedited
  ATAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGATTTGGTCCTGATGTCCTTTGAGGCTTCTTTAGCTCTAGTTCTCT
  GACACTTCAGCCTATATCACAGCTAACTTCTTCAGTCTCATCTATTCCTTATGCTCCAGC
  CCCTGGCAATTTGCCTCAAGATGGGGGTTTGAAAATAACTTTACCTGACTCAAGGAGTGT
  CTGGAGCACCTCCTAGTCTAAGTCTGCAAGCTCCAGTTCTTGCCTAAAACCATGCCAGTG
  GCCACCCTTGGGCTCAGACAGCTCTGGGCCTTTTGACCACAAGCCAGCCCCTCGCCCTCT
  CTGTGGCATAGTCTTCTCTGCCCCAGGACTGCAGGGCGGCTTCCTCCAAGGCTTCCAAGG
  CTCAAAAGAAATTTGGCTCCATCCAAGAAGGCTCCAGCTCCCCTACTGGCCCCTGGCTCA
  GGCCCACACCCCTGGCCAGGCCCAGAGAGTGTGTCTCAGGAGAATTCAATGGCTCTAGAG
  AGACACACAGAAAGTTTGGCATTTGGAAATTTCAAGGATGTATGTATGCTCACGTATGGA
  GCAGGTTGTCCTGGTCCCTGNGTGCAGGNAAGTGGGCTGCAGGGAAGTGGATTGCAGGGG
  AGCTTGAGGAATATAAGGAGCGGNNGGTGGAGACTCANGCTATGGACCAGGACAGCCCCA
  AGGTGGGGAAGACCTGGCCTTAGTCGTCCTCAGCCTAGGGCAGGGCAGTGAAGAAAGCTC
  TCCCCGCCTCCTGCCTGTATGACCCAGATAGCCTCCCCAGCCCGCATCTTTATGTGTGGT
  CTCCACCATCCTCATGGGGCACTTTNCTAGCCTGTCTCCCACACTGTGCAGGCCTCGAGA
  GACCCAGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK056725 unedited
  GTAATACTATGNNACCGCGGCCGCAATTCTANGATCGGTTTTTTTTTTTTTTTTTTCAGN
  ATTTAACCATGGTTTATTGCAGACCTATTGTGTGCCAGGCATGAGCCCAGTGCTCCCACA
  AACAGCGTCTCCAGGTACGAGAAGTGTAATTTCGACCCTGGAACACCTTCCTGCACTCAG
  ACAGAAGCATGTGCAGACTGCTCTCAGCAACGCCACCTCCTTCACTCCCTGGCCATTGGG
  CCCCCTTCTCTCTACCTGTCCCCTGTCACTCCACTTCCTGCCCCAGGCCTTCTCTTAGGG
  ATTTTCCAGGCTTTGGGGAGCTGCTGCTTTCAGTTTCTGGCTGTGGGGTCTCAGCAGGAG
  TTTGTTCCAAAACCCAGTAGAAATAAAATCCTTCAAGAGGGAGATGATTCGTGGGCTGGG
  TGGATTCCAGTGACCAGAGGACGTGCAAACTCTGCCCTAATGATCTGGGAACCTAGCCCA
  TCCATTGAGCTTTCTGTTTTCACCCAGTTTCACTTCCTGGTTCTCTTCCGAGCCTTGCAC
  AATGCTGGGAGACAGGCCTAGAAAAGTGCCACCATGAGGATGGTGGAAGACACACATAAT
  ATGCCGGCCTGGGGAGGCTACTCTGGGTCATTACAGCAGGAGCGGGGAGAGCTTTCTTCA
  CTGCCCTGCCCCTTAGCTGAAGACGACTAAAGGCAGGTCTTTCCACCTTTGGGGCTGTCC
  TTGTGCATAGCCTGGAGCTCCCCCCCCGCTCCTTAAATTTCTTAAGCTCCCCTCCAATCC
  ACTTTTCTTGAAGGCCAATTTTCTTGGGCCCCAGGGACCAGGAAAAACCTGGTTCTTACG
  GGAGGAAACATACCCCCCTTGGAATTTCCAAAGGCCAACCTTTTTTGGTGGGTTTCTAAA
  GGCCTGGAAATTTCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: AK056725
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK056725.1
• RefSeq Size: 1960 bp
• RefSeq ORF: 1960 bp
• Locus ID: 81788
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.[UniProtKB/Swiss-Prot Function]