ZCRB1 (NM_033114) Human Untagged Clone

CAT#: SC101692

ZCRB1 (untagged)-Human zinc finger CCHC-type and RNA binding motif 1 (ZCRB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZCRB1
• Synonyms: MADP-1; MADP1; RBM36; SNRNP31; ZCCHC19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101692 sequence for NM_033114 edited (data generated by NextGen Sequencing)
  ATGAGTGGTGGATTGGCTCCAAGTAAGAGCACAGTGTATGTATCCAACTTGCCTTTTTCC
  CTGACAAACAATGACTTGTACCGGATATTTTCCAAGTATGGCAAAGTTGTAAAGGTTACC
  ATCATGAAAGATAAAGATACCAGGAAGAGTAAAGGGGTTGCATTTATTTTATTTTTGGAT
  AAAGACTCTGCACAAAACTGTACCAGGGCAATAAACAACAAACAGTTATTTGGTAGAGTG
  ATAAAAGCAAGCATTGCTATTGACAATGGAAGAGCAGCTGAGTTCATCCGAAGGCGAAAC
  TACTTTGATAAATCTAAGTGTTATGAATGTGGGGAAAGTGGACACTTAAGTTATGCCTGT
  CCGAAAAATATGCTCGGAGAACGTGAGCCTCCAAAGAAGAAAGAAAAAAAGAAAAAAAAG
  AAAGCTCCTGAACCAGAAGAAGAAATTGAGGAAGTAGAAGAAAGTGAAGATGAAGGGGAG
  GATCCTGCTCTTGACAGCCTCAGTCAGGCCATAGCATTCCAGCAAGCCAAAATTGAAGAA
  GAACAAAAAAAATGGAAACCCAGTTCAGGAGTCCCCTCAACATCAGATGATTCAAGACGC
  CCAAGGATAAAGAAAAGCACATATTTCAGTGATGAGGAAGAACTTAGTGATTAA
  
  Clone variation with respect to NM_033114.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033114 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCTGCTATTATCTG
  CTCTCCGCGCCTGACCCCTCCCAGGACTCGTGATGCCAAGGCCGCTGCGAGCGGCTACGA
  AGAGTCGGGGTTGAGCCCCAGCTGAGCCGAGGGCTCGCACTCTTCTGGTCTCCCAGGCCC
  AACCCACCTGAAGAAATGAGTGGTGGATTGGCTCCAAGTAAGAGCACAGTGTATGTATCC
  AACTTGCCTTTTTCCCTGACAAACAATGACTTGTACCGGATATTTTCCAAGTATGGCAAA
  GTTGTAAAGGTTACCATCATGAAAGATAAAGATACCAGGAAGAGTAAAGGGGTTGCATTT
  ATTTTATTTTTGGATAAAGACTCTGCACAAAACTGTACCAGGGCAATAAACAACAAACAG
  TTATTTGGTAGAGTGATAAAAGCAAGCATTGCTATTGACAATGGAAGAGCAGCTGAGTTC
  ATCCGAAGGCGAAACTACTTTGATAAATCTAAGTGTTATGAATGTGGGGAAAGTGGACAC
  TTAAGTTATGCCTGTCCGAAAAATATGCTCGGAGAACGTGAGCCTCCAAAGAAGAAAGAA
  AAAAAGAAAAAAAAGAAAGCTCCTGAACCAGAAGAAGAAATTGAGGAAGTAGAAGAAAGT
  GAAGATGAAGGGGAGGATCCTGCTCTTGACAGCCTCAGTCAGGCCATAGCATTCCAGCAA
  GCCAAAATTGAAGAAGAACAAAAAAAATGGAAACCCAGTTCAGGAGTCCCCTCACATCAG
  ATGATTCAAGACGCCCAAGGATAAAGAANAGCACATATTTCAGTGATGAGGAAGAACTTA
  GTGATTAAATCTTGCCCCAGCACAGTATAAAATCAAGATTGGTAGTACAATCTTGAGAGC
  TTATTTAATAAAAAGAAAATATACCTTCGGTATACATTATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033114 unedited
  ACTGTTTCACTCTGCACCGCGCGCCGCAATCTACGATCGAGTTTTTTTTTTTTTTTTTTA
  AAAACAAAATTTCTCTTTGAAAAGTTTATTAATATAAGTAACAATAAATCATTCAACTTT
  TCGGTCTTCAGGAAGTTTGTTTTAAGGATTAATTTCAGAAGTCCTCATGTAAACAAATCA
  GGCATGAATAAAGCCCTTATAATGAACTTTTCAAATAAATTTTTAAAATATCTTTTTTCT
  TGGGATGACAATAATAGTATTAACATGATAGTATTAATTTTTCTTATTTTTATTAAAATT
  AGCTCTTCAAGATTGTTACTAACAAATCTTGATTTTTATTACTGTGCTGGGGCAAGATTT
  TAATCACTAAGTTCTTCCTCATCACTGAAATATGTGCTTTTCTTTATCCTTGGGCGTCTT
  GAATCATCTGATGTTGAGGGGACTCCTGAACTGGGTTTCCATTTTTTTTGTTCTTCTTCA
  ATTTTGGCTTGCTGGAATGCTATGGCCTGACTGAGGCTGTCAAGAGCAGGATCCTCCCCT
  TCATCTTCACTTTCTTCTACTTCCTCAATTCCTTCTTCTGGCTCAGGAGCTTTCTTTTTT
  TTCTTCTCTTCTTTCTTCCTTGGAGGCTCACGTTCTCCGAGCATATTTTTCGGACAGGCA
  TAACTTAAGTGTCCACTTTCCCCACATTCATAACACTTACATTTATCAAAGCAGCTTCCG
  CCTTCGGATGAACTCAGCTGCTCCTTCATTGTCAATAGCATGCTTGCTTCTATCCATCTC
  ACCAAAAACTGTTTGTTGCTTATTGCCCTGTCCCAGTTTTGCGCCAAGTTTTTATCCCAA
  AAAAAATAAAGCCACCCCTTTACTCCTTCCGGGATCTTATCTTCATGAGGGAACCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033114
• Insert Size: 1160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033114.3, NP_149105.3
• RefSeq Size: 1844 bp
• RefSeq ORF: 654 bp
• Locus ID: 85437
• UniProt ID: Q8TBF4
• Cytogenetics: 12q12
• Domains: RRM, zf-CCHC, PAP_assoc, NTP_transf_2, RRM_1
• Gene Summary: Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq, Jul 2008]