ZCRB1 (NM_033114) Human Tagged ORF Clone

CAT#: RG205492

ZCRB1 (tGFP-tagged) - Human zinc finger CCHC-type and RNA binding motif 1 (ZCRB1)

Product Images

Circular map for RG205492

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZCRB1
• Synonyms: MADP-1; MADP1; RBM36; SNRNP31; ZCCHC19
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205492 representing NM_033114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGTGGATTGGCTCCAAGTAAGAGCACAGTGTATGTATCCAACTTGCCTTTTTCCCTGACAAACA
  ATGACTTGTACCGGATATTTTCCAAGTATGGCAAAGTTGTAAAGGTTACCATCATGAAAGATAAAGATAC
  CAGGAAGAGTAAAGGGGTTGCATTTATTTTATTTTTGGATAAAGACTCTGCACAAAACTGTACCAGGGCA
  ATAAACAACAAACAGTTATTTGGTAGAGTGATAAAAGCAAGCATTGCTATTGACAATGGAAGAGCAGCTG
  AGTTCATCCGAAGGCGAAACTACTTTGATAAATCTAAGTGTTATGAATGTGGGGAAAGTGGACACTTAAG
  TTATGCCTGTCCGAAAAATATGCTCGGAGAACGTGAGCCTCAAAAGAAGAAAGAAAAAAAGAAAAAAAAG
  AAAGCTCCTGAACCAGAAGAAGAAATTGAGGAAGTAGAAGAAAGTGAAGATGAAGGGGAGGATCCTGCTC
  TTGACAGCCTCAGTCAGGCCATAGCATTCCAGCAAGCCAAAATTGAAGAAGAACAAAAAAAATGGAAACC
  CAGTTCAGGAGTCCCCTCAACATCAGATGATTCAAGACGCCCAAGGATAAAGAAAAGCACATATTTCAGT
  GATGAGGAAGAACTTAGTGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205492 representing NM_033114
  Red=Cloning site Green=Tags(s)
  
  MSGGLAPSKSTVYVSNLPFSLTNNDLYRIFSKYGKVVKVTIMKDKDTRKSKGVAFILFLDKDSAQNCTRA
  INNKQLFGRVIKASIAIDNGRAAEFIRRRNYFDKSKCYECGESGHLSYACPKNMLGEREPQKKKEKKKKK
  KAPEPEEEIEEVEESEDEGEDPALDSLSQAIAFQQAKIEEEQKKWKPSSGVPSTSDDSRRPRIKKSTYFS
  DEEELSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033114
• ORF Size: 651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033114.2
• RefSeq Size: 1844 bp
• RefSeq ORF: 654 bp
• Locus ID: 85437
• UniProt ID: Q8TBF4
• Cytogenetics: 12q12
• Domains: RRM, zf-CCHC, PAP_assoc, NTP_transf_2, RRM_1
• Gene Summary: Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq, Jul 2008]