Ankrd50 (NM_001191606) Rat Tagged ORF Clone

CAT#: RR217621

Ankrd50 (myc-DDK-tagged) - Rat ankyrin repeat domain 50 (Ankrd50)

Product Images

Circular map for RR217621

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankrd50
• Synonyms: RGD1311665
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217621 representing NM_001191606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAATCCTTGGGAAGAGAAAGTCTGCAAAATGGCCCAGACGAGTCTCCTGCAAGGGAAGCAGTTCT
  ACTGTCGGGAATGGGTTTTCCACAAACTTCAGCACTGCCTCCAGGAGAAATCAAGCCGCTGTTTCAGTGC
  AGCCAATGCACAGTCCCTGGTCGGGGATGCTGGGAATAATGCTAGTGCCCTCTCTGGAAAGGGAGCCTCC
  TGGGGCGTGTTGCTGGTCGGAGGGCCTGGCAGTGGGAAGACAGCCCTGTGCACTGAGCTCCTGTGGCCCA
  GTTCCCCCGCCAGCCTGCAGAGAGGTCTGCATCGGCAGGCTTTGGCTTTCCACTTCTGCAAAGCCCAGGA
  CTCGGACACACTGTGTGTCGGGGGGTTTATCAGAGGTCTGGTAGCCCAGATCTGCCAAAGCGGACTCCTC
  CAAGGTTATGAGGACAAGCTAAGAGATCCAGCCGTCCAAAGTCTCCTAGAGCCCGGAGAGTGTGAGAGGA
  ACCCAGCGGAAGCATTTAAAAGGTGTGTGCTGCTCCCACTCTTGGGGATGAAGCCACCCCCGCAAAGCCT
  CTACCTGTTGGTTGACTCCGTGGATGAAGGCTGCAATGTGGTGGAAGGGGAGCAGACGTCACCCAACCTC
  TCCGGGACTGTGGCAGAGCTCTTGGCTGGTCACCACGAGTTCTTTCCCCCATGGCTCCTGCTTCTCTGCT
  CTGCCCGGAAGCAGAGCAGAGCTGTCACTAAAATGTTTACTGGGTTCCGGAAGATCAGTTTGGATGACCT
  CCGGAAGGCGTATATCGTCAAGGATGTACAGCAGTACATCCTCCACCGCCTGGATCAAGAGGAGGCACTC
  CGGCAGCATCTCACCAAGGAGACGGCTGAGACTCTGAACCAGCTGCACATCAAGAGCAGCGGGTGCTTCT
  TGTACCTGGAGCGAGTGCTAGATGGAGTTGTAGAGAATTTCATCATGCTTCGAGAGATCCGTGACATCCC
  CGGAACTCTGAATGGTCTCTACCTCTGGCTCTGCCAGAGACTTTTTGTCCGAAAACAGTTTGCCAAAGTT
  CAGCCCATTCTGAATGTGATCCTCGCTGCCTGCCGGCCCCTGACCATGACAGAATTATACCATGCTGTGT
  GGACCAAAAATATGTCTCTAACCTTGGAAGACTTTCAGCGCAAGCTAGACGTCCTCTCCAAGCTCTTGGT
  GGATGGGTTGGGCAGCACCAAAATCCTGTTTCACCACAGCTTTGCTGAGTGGCTCTTGGATGTGAAGCAC
  TGCACCCAGAAATACCTGTGTAACGCTGCAGAAGGACACAGGATGCTGGCCATGAGCTACACCTGCCAGG
  CCCGGAACCTGACCCCACTGGAAGCACAAGAATTTGCCTTGCACTTGATTAATTCAAACTTGCAGCTGGA
  GCCAGCCGAACTGGCTCTGTGGATGATATGGAATGGTACCCCAGTCAAAGACTCACTGTCCACCTTGATA
  CCCAAGGAGCAAGAGGTCCTGCAGCTGCTGATCCGAGCTGGTGCTCATGTCAACAGTGAGGATGATCGCA
  CCTCCTGTATTGTCCGGCAAGCCCTCGAGAGAGAGGACTCAATTCGGACTTTACTGGATAGTGGAGCTTC
  TGTCAATCAGTGTGATTCCAATGGGAGAACTCTGCTGGCCAATGCCGCATACAGTGGCAGTCTGGATGTT
  GTGAACTTGCTTGTCTCCAGGGGAGCCGATTTAGAAATCGAAGACACCCATGGACACACGCCACTTACTC
  TAGCTGCTAGACAAGGGCACACAAAGGTGGTTAACTGTCTAATTGGGTGTGGAGCCAATATTAATCATAC
  AGATCAGGACGGTTGGACAGCACTGAGGTCTGCTGCTTGGGGGGGCCACACAGAAGTGGTGTCTGCTCTG
  CTTTACGCTGGCGTAAAGGTAGACTGTGCAGATGCGGACAGCCGGACTGCTCTGCGTGCAGCAGCCTGGG
  GTGGCCACGAGGACATCGTCCTGAACCTGCTACAACATGGTGCAGAGGTCAACAAAGCCGACAATGAAGG
  GAGGACTGCACTCATAGCTGCAGCATATATGGGCCACAGAGAGATCGTAGAGCACCTGCTGGACCATGGT
  GCAGAGGTAAACCATGAGGACGTGGATGGCAGAACAGCGCTCTCTGTGGCTGCGCTCTGTGTGCCGGCAA
  GCAAAGGGCATGCGTCAGTCGTGAGCCTCTTGATTGATCGAGGGGCTGAGGTAGATCACTGTGATAAGGA
  TGGCATGACCCCCCTGCTGGTCGCTGCCTACGAAGGTCATGTGGATGTTGTAGACCTGCTTCTAGAAGGA
  GGAGCAGATGTTGATCACACCGATAACAATGGACGGACACCCCTTCTGGCTGCAGCTTCCATGGGGCATG
  CTTCCGTGGTCAACACACTTCTGTTCTGGGGCGCAGCCGTGGACAGCATTGACAGCGAAGGCAGGACAGT
  TCTCAGCATAGCTTCAGCACAGGGGAATGTGGAGGTGGTGCGCACTCTCCTGGACAGAGGCTTGGACGAG
  AGCCACCGAGATGATGCTGGCTGGACACCTCTACACATGGCAGCTTTTGAGGGTCACAGGTTAATATGTG
  AAGCTCTCATTGAACAAGGTGCTAGAACAAATGAGATTGACAATGATGGGCGCATCCCCTTCATTTTGGC
  CTCCCAAGAGGGCCATTATGATTGTGTCCAGATTCTGCTGGAGAACAAATCCAACATTGATCAGAGAGGC
  TATGACGGGAGAAATGCATTACGAGTTGCTGCCCTGGAAGGACACCGGGACATCGTGGAATTACTCTTTA
  GCCATGGGGCTGATGTGAACTACAAAGATGCCGATGGGCGGCCCACACTTTACATATTGGCCTTAGAAAA
  CCAGCTTACAATGGCCGAGTACTTTCTAGAAAATGGTGCAAACGTGGAGGCCAGTGATGCAGAGGGAAGG
  ACAGCGCTGCACGTCTCCTGCTGGCAGGGTCACGTGGAGATGGTACGGGTACTTATCGCCTGCCATGCCG
  ATGTCAATGCAGCAGACAATGAGAAGCGCTCAGCCTTGCAGTCTGCGGCCTGGCAGGGCCATGTCAAAGT
  GGTCCAGCTTCTGATAGAGCATGGTGCCGTGGTGGACCACACGTGCAACCAAGGAGCCACAGCCCTCTGC
  ATCGCAGCCCAGGAGGGACATGTAGACGTAGTGCAGGTTTTGCTGGAGCACGGTGCTGATCCCAATCATG
  CCGACCAATTTGGACGTACTGCCATGAGGGTGGCAGCCAAAAACGGGCATTCTCAGATCATTAAGTTGTT
  AGAAAAATACGGTGCTTCCAGTCTGAATGGCTGCTCCCCTTCCCCTGTCCACACAATGGAGCAGAAGCCT
  CCACAGTCGACACCATCCAAGATGCAGTCTCTTACCATCAGGTCCAACAGCTCTGGTGGTACAGGCGGAG
  GGGATCTGCAGCCTTCCCTCCGGGGCTTGCCCAATGGGCCGGCTCATGCCTTCAGCTCTCCATCAGAGTC
  TCCAGACTCAACCGTGGATCGGCAAAAGTCGTCTCTGTCCAACAATTCTCTGAAAAGCTCCAAAAACTCA
  TCTCTGAGAACAACGTCCTCCACAGCCACGGCTCAGACGGTGCCCATTGACAGTTTCCATAGCCTGTCGT
  TCACGGAGCAGATCCAACAGCACTCGCTGCCTCGCAGCAGGAGCAGGCAGTCTGTTGTGTCCCCCTCTTC
  CACAACCCAGTCCTTAGGACACAGCCATAATTCTCCCAGTAGTGAGTTCGAATGGAGCCAGGTGAAACCA
  AGTCTGAAGTCAACAAAATCAAATAAAGGAGGCAAGTCAGAAAACTCCAGCAAGTCTGGGTCAGCTGGGA
  AGAAAGCTAAACAAAACAATTCCTCACAGCCAAAGGTTCTAGAATATGAAATGACTCAGTTTGACAAAAG
  AGGACCTGTTGCCAAGTCTGGGAGTAGCCCGCCTAAGCAGATGCCAGCTGAATCTCAGTGTAAAATCATG
  GTCCCTTCATCTCAGGACAGCAACCGTGCCCAGCCACAGTTTCTCATCCACCAGCAGGGCGGCGAGCAGA
  AGAGGAGGAATGGGATAATGACCAACCCCAATTACCATCTGCAGAGCAACCAGGTGTTCCTGGGCAGGGT
  CTCCGTCCCTCGAACACTGCAGGAGCGAGGGCATCAGGAGGTGCTGGAGGGCTTCCCCCCCTCCGAGACA
  GAGCTGAACCTTAAACAAGCGCTGAAGCTCCAGATTGAGGGCTCTGACCCCAGCTTCAACTACAAGAAGG
  AAACGCCCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217621 representing NM_001191606
  Red=Cloning site Green=Tags(s)
  
  MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKSSRCFSAANAQSLVGDAGNNASALSGKGAS
  WGVLLVGGPGSGKTALCTELLWPSSPASLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICQSGLL
  QGYEDKLRDPAVQSLLEPGECERNPAEAFKRCVLLPLLGMKPPPQSLYLLVDSVDEGCNVVEGEQTSPNL
  SGTVAELLAGHHEFFPPWLLLLCSARKQSRAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEAL
  RQHLTKETAETLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKV
  QPILNVILAACRPLTMTELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGSTKILFHHSFAEWLLDVKH
  CTQKYLCNAAEGHRMLAMSYTCQARNLTPLEAQEFALHLINSNLQLEPAELALWMIWNGTPVKDSLSTLI
  PKEQEVLQLLIRAGAHVNSEDDRTSCIVRQALEREDSIRTLLDSGASVNQCDSNGRTLLANAAYSGSLDV
  VNLLVSRGADLEIEDTHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSAL
  LYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHG
  AEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEG
  GADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE
  SHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRG
  YDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGR
  TALHVSCWQGHVEMVRVLIACHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALC
  IAAQEGHVDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKP
  PQSTPSKMQSLTIRSNSSGGTGGGDLQPSLRGLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNS
  SLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSVVSPSSTTQSLGHSHNSPSSEFEWSQVKP
  SLKSTKSNKGGKSENSSKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPVAKSGSSPPKQMPAESQCKIM
  VPSSQDSNRAQPQFLIHQQGGEQKRRNGIMTNPNYHLQSNQVFLGRVSVPRTLQERGHQEVLEGFPPSET
  ELNLKQALKLQIEGSDPSFNYKKETPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191606
• ORF Size: 4281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191606.1, NP_001178535.1
• RefSeq Size: 4831 bp
• RefSeq ORF: 4284 bp
• Locus ID: 294988
• Cytogenetics: 2q25
• MW: 155.6 kDa