Ankrd50 (NM_001191606) Rat Untagged Clone

CAT#: RN217621

Ankrd50 (untagged) - Rat ankyrin repeat domain 50 (Ankrd50)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ankrd50
• Synonyms: RGD1311665
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217621 representing NM_001191606
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAATCCTTGGGAAGAGAAAGTCTGCAAAATGGCCCAGACGAGTCTCCTGCAAGGGAAGCAGTTCT
  ACTGTCGGGAATGGGTTTTCCACAAACTTCAGCACTGCCTCCAGGAGAAATCAAGCCGCTGTTTCAGTGC
  AGCCAATGCACAGTCCCTGGTCGGGGATGCTGGGAATAATGCTAGTGCCCTCTCTGGAAAGGGAGCCTCC
  TGGGGCGTGTTGCTGGTCGGAGGGCCTGGCAGTGGGAAGACAGCCCTGTGCACTGAGCTCCTGTGGCCCA
  GTTCCCCCGCCAGCCTGCAGAGAGGTCTGCATCGGCAGGCTTTGGCTTTCCACTTCTGCAAAGCCCAGGA
  CTCGGACACACTGTGTGTCGGGGGGTTTATCAGAGGTCTGGTAGCCCAGATCTGCCAAAGCGGACTCCTC
  CAAGGTTATGAGGACAAGCTAAGAGATCCAGCCGTCCAAAGTCTCCTAGAGCCCGGAGAGTGTGAGAGGA
  ACCCAGCGGAAGCATTTAAAAGGTGTGTGCTGCTCCCACTCTTGGGGATGAAGCCACCCCCGCAAAGCCT
  CTACCTGTTGGTTGACTCCGTGGATGAAGGCTGCAATGTGGTGGAAGGGGAGCAGACGTCACCCAACCTC
  TCCGGGACTGTGGCAGAGCTCTTGGCTGGTCACCACGAGTTCTTTCCCCCATGGCTCCTGCTTCTCTGCT
  CTGCCCGGAAGCAGAGCAGAGCTGTCACTAAAATGTTTACTGGGTTCCGGAAGATCAGTTTGGATGACCT
  CCGGAAGGCGTATATCGTCAAGGATGTACAGCAGTACATCCTCCACCGCCTGGATCAAGAGGAGGCACTC
  CGGCAGCATCTCACCAAGGAGACGGCTGAGACTCTGAACCAGCTGCACATCAAGAGCAGCGGGTGCTTCT
  TGTACCTGGAGCGAGTGCTAGATGGAGTTGTAGAGAATTTCATCATGCTTCGAGAGATCCGTGACATCCC
  CGGAACTCTGAATGGTCTCTACCTCTGGCTCTGCCAGAGACTTTTTGTCCGAAAACAGTTTGCCAAAGTT
  CAGCCCATTCTGAATGTGATCCTCGCTGCCTGCCGGCCCCTGACCATGACAGAATTATACCATGCTGTGT
  GGACCAAAAATATGTCTCTAACCTTGGAAGACTTTCAGCGCAAGCTAGACGTCCTCTCCAAGCTCTTGGT
  GGATGGGTTGGGCAGCACCAAAATCCTGTTTCACCACAGCTTTGCTGAGTGGCTCTTGGATGTGAAGCAC
  TGCACCCAGAAATACCTGTGTAACGCTGCAGAAGGACACAGGATGCTGGCCATGAGCTACACCTGCCAGG
  CCCGGAACCTGACCCCACTGGAAGCACAAGAATTTGCCTTGCACTTGATTAATTCAAACTTGCAGCTGGA
  GCCAGCCGAACTGGCTCTGTGGATGATATGGAATGGTACCCCAGTCAAAGACTCACTGTCCACCTTGATA
  CCCAAGGAGCAAGAGGTCCTGCAGCTGCTGATCCGAGCTGGTGCTCATGTCAACAGTGAGGATGATCGCA
  CCTCCTGTATTGTCCGGCAAGCCCTCGAGAGAGAGGACTCAATTCGGACTTTACTGGATAGTGGAGCTTC
  TGTCAATCAGTGTGATTCCAATGGGAGAACTCTGCTGGCCAATGCCGCATACAGTGGCAGTCTGGATGTT
  GTGAACTTGCTTGTCTCCAGGGGAGCCGATTTAGAAATCGAAGACACCCATGGACACACGCCACTTACTC
  TAGCTGCTAGACAAGGGCACACAAAGGTGGTTAACTGTCTAATTGGGTGTGGAGCCAATATTAATCATAC
  AGATCAGGACGGTTGGACAGCACTGAGGTCTGCTGCTTGGGGGGGCCACACAGAAGTGGTGTCTGCTCTG
  CTTTACGCTGGCGTAAAGGTAGACTGTGCAGATGCGGACAGCCGGACTGCTCTGCGTGCAGCAGCCTGGG
  GTGGCCACGAGGACATCGTCCTGAACCTGCTACAACATGGTGCAGAGGTCAACAAAGCCGACAATGAAGG
  GAGGACTGCACTCATAGCTGCAGCATATATGGGCCACAGAGAGATCGTAGAGCACCTGCTGGACCATGGT
  GCAGAGGTAAACCATGAGGACGTGGATGGCAGAACAGCGCTCTCTGTGGCTGCGCTCTGTGTGCCGGCAA
  GCAAAGGGCATGCGTCAGTCGTGAGCCTCTTGATTGATCGAGGGGCTGAGGTAGATCACTGTGATAAGGA
  TGGCATGACCCCCCTGCTGGTCGCTGCCTACGAAGGTCATGTGGATGTTGTAGACCTGCTTCTAGAAGGA
  GGAGCAGATGTTGATCACACCGATAACAATGGACGGACACCCCTTCTGGCTGCAGCTTCCATGGGGCATG
  CTTCCGTGGTCAACACACTTCTGTTCTGGGGCGCAGCCGTGGACAGCATTGACAGCGAAGGCAGGACAGT
  TCTCAGCATAGCTTCAGCACAGGGGAATGTGGAGGTGGTGCGCACTCTCCTGGACAGAGGCTTGGACGAG
  AGCCACCGAGATGATGCTGGCTGGACACCTCTACACATGGCAGCTTTTGAGGGTCACAGGTTAATATGTG
  AAGCTCTCATTGAACAAGGTGCTAGAACAAATGAGATTGACAATGATGGGCGCATCCCCTTCATTTTGGC
  CTCCCAAGAGGGCCATTATGATTGTGTCCAGATTCTGCTGGAGAACAAATCCAACATTGATCAGAGAGGC
  TATGACGGGAGAAATGCATTACGAGTTGCTGCCCTGGAAGGACACCGGGACATCGTGGAATTACTCTTTA
  GCCATGGGGCTGATGTGAACTACAAAGATGCCGATGGGCGGCCCACACTTTACATATTGGCCTTAGAAAA
  CCAGCTTACAATGGCCGAGTACTTTCTAGAAAATGGTGCAAACGTGGAGGCCAGTGATGCAGAGGGAAGG
  ACAGCGCTGCACGTCTCCTGCTGGCAGGGTCACGTGGAGATGGTACGGGTACTTATCGCCTGCCATGCCG
  ATGTCAATGCAGCAGACAATGAGAAGCGCTCAGCCTTGCAGTCTGCGGCCTGGCAGGGCCATGTCAAAGT
  GGTCCAGCTTCTGATAGAGCATGGTGCCGTGGTGGACCACACGTGCAACCAAGGAGCCACAGCCCTCTGC
  ATCGCAGCCCAGGAGGGACATGTAGACGTAGTGCAGGTTTTGCTGGAGCACGGTGCTGATCCCAATCATG
  CCGACCAATTTGGACGTACTGCCATGAGGGTGGCAGCCAAAAACGGGCATTCTCAGATCATTAAGTTGTT
  AGAAAAATACGGTGCTTCCAGTCTGAATGGCTGCTCCCCTTCCCCTGTCCACACAATGGAGCAGAAGCCT
  CCACAGTCGACACCATCCAAGATGCAGTCTCTTACCATCAGGTCCAACAGCTCTGGTGGTACAGGCGGAG
  GGGATCTGCAGCCTTCCCTCCGGGGCTTGCCCAATGGGCCGGCTCATGCCTTCAGCTCTCCATCAGAGTC
  TCCAGACTCAACCGTGGATCGGCAAAAGTCGTCTCTGTCCAACAATTCTCTGAAAAGCTCCAAAAACTCA
  TCTCTGAGAACAACGTCCTCCACAGCCACGGCTCAGACGGTGCCCATTGACAGTTTCCATAGCCTGTCGT
  TCACGGAGCAGATCCAACAGCACTCGCTGCCTCGCAGCAGGAGCAGGCAGTCTGTTGTGTCCCCCTCTTC
  CACAACCCAGTCCTTAGGACACAGCCATAATTCTCCCAGTAGTGAGTTCGAATGGAGCCAGGTGAAACCA
  AGTCTGAAGTCAACAAAATCAAATAAAGGAGGCAAGTCAGAAAACTCCAGCAAGTCTGGGTCAGCTGGGA
  AGAAAGCTAAACAAAACAATTCCTCACAGCCAAAGGTTCTAGAATATGAAATGACTCAGTTTGACAAAAG
  AGGACCTGTTGCCAAGTCTGGGAGTAGCCCGCCTAAGCAGATGCCAGCTGAATCTCAGTGTAAAATCATG
  GTCCCTTCATCTCAGGACAGCAACCGTGCCCAGCCACAGTTTCTCATCCACCAGCAGGGCGGCGAGCAGA
  AGAGGAGGAATGGGATAATGACCAACCCCAATTACCATCTGCAGAGCAACCAGGTGTTCCTGGGCAGGGT
  CTCCGTCCCTCGAACACTGCAGGAGCGAGGGCATCAGGAGGTGCTGGAGGGCTTCCCCCCCTCCGAGACA
  GAGCTGAACCTTAAACAAGCGCTGAAGCTCCAGATTGAGGGCTCTGACCCCAGCTTCAACTACAAGAAGG
  AAACGCCCTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001191606
• Insert Size: 4284 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191606.1, NP_001178535.1
• RefSeq Size: 4831 bp
• RefSeq ORF: 4284 bp
• Locus ID: 294988
• Cytogenetics: 2q25