Ndst4 (NM_001191849) Rat Tagged ORF Clone

CAT#: RR217283

Ndst4 (myc-DDK-tagged) - Rat N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (Ndst4)

Product Images

Circular map for RR217283

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ndst4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217283 representing NM_001191849
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTATTGTAAAGTTTCGGAGAAGCTTTCGAACACTGATTGTTCTCTTAGCTACCTTTTGTTTGG
  TGAGCATCGTCATTTCTGCCTACTTCCTGTACTCTGGCTACAAACAGGAAATGACCCTTGTCGAAACTAC
  TGCAGAAGCAGAGTGCGCTGACATCAAAAACCTTCCTTATCGGTCTATAGAACTGAAAACCATCAAGCCT
  ATTGATACATCCAAAACTGATCCCACTGTCCTTCTCTTTGTAGAGAGCCAGTATTCGCAACTTGGTCAAG
  ATATTATAGCAATCTTGGAGTCCAGCCGGTTTCAGTACCAGATGGTCATTGCTCCAGGCAAGGGAGACAT
  ACCTCCTCTTACCGATAGTGGCAAGGGGAAGTACACATTGATTATTTACGAAAACATTTTGAAGTACGTC
  AGCATGGACTCATGGAACCGGGAGCTTTTAGAAAAATACTGTATAGAATACAGTGTTAGCATTATTGGTT
  TTCATAAAGCCAATGAGAACAGCTTGCCGACTACACAACTGAAAGGGTTTCCTTTGAACTTATTCAATAA
  TGTAGCTCTGAAGGACTGCTCTGTGAACCCCCAGTCTCCTCTGCTTCATATTACCAAAGGCCCCAAAGTT
  GAAAAGGGACCTCTTCCTGGGGAAGACTGGACAATTTTCCAATATAACCATTCTACCTACCAGCCAGTGC
  TTTTAACTGAGTTACAAACAGAAAAGTCCCTCTCTTTCTTGTCCAGCCAAACACTCTATGCGACAATAAT
  TCAGGATCTGGGGCTTCATGATGGGATCCAGCGAGTTCTTTTTGGTAACAATTTGAACTTTTGGTTGCAC
  AAGCTCATTTTCATAGACGCCATCTCTTTCTTGTCGGGCAAGAGGCTGACACTATCCTTGGACAGGTACA
  TCCTTGTGGACATTGATGACATATTTGTGGGGAAGGAGGGCACAAGGATGAATGTCAAAGATGTGAAGGC
  ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG
  AAGTTTTACCACACAGGGACTGAAGAAGAAGATGCAGGGGATGACCTTCTGCTGAGGTCTGTGGATGAGT
  TCTGGTGGTTTCCTCATATGTGGAGTCACATGCAGCCCCACCTGTTCCACAATGAGTCTTCTTTAGTGGA
  ACAGATGATTCTTAACAAAGAATTTGCCCTGGAACATGGAATTCCGATCAACCTGGGCTATGCAGTGGCC
  CCACATCATTCAGGAGTCTACCCAGTCCATATTCAGCTTTATGCAGCTTGGAAGAAGGTCTGGGGTATTC
  AGGTCACCAGCACTGAAGAATATCCACATCTAAAGCCTGCACGGTACCGGAAGGGTTTCATTCACAACAA
  CATCATGGTCCTCCCTCGACAGACCTGTGGGCTGTTCACGCATACTATTTTCTACAAGGAGTATCCAGGA
  GGACCTCAAGAATTGGATAAAAGTATCAAAGGAGGTGAACTTTTCCTCACAATTCTTCTAAATCCAATCA
  GCATTTTCATGACGCATCTGTCTAACTACGGCAATGACCGCCTCGGCTTATACACCTTTGTGAACTTGGC
  CAACTTTGTGCACAGCTGGACCAACTTGAAACTGCAGACCCTGCCACCAGTGCAACTGGCCCATAAGTAC
  TTCGAACTCTTCCCTGAGCAGAAAGACCCTCTCTGGCAGAATCCATGTGATGATAAACGACACAAGGACA
  TCTGGTCCAGGGAGAAAACTTGTGATCATTTACCCAAATTCCTTGTGATTGGGCCACAGAAAACAGGAAC
  AACCGCGCTTTATTTATTTCTTCTTATGCACCCTTCCATCGTCAGCAATCTCCCTAGCCCGAAAACCTTC
  GAAGAAGTTCAATTTTTTAACGGCAACAACTATCACAAGGGAATTGAGTGGTATATGGACTTCTTTCCCA
  CTCCTTCCAACATTACCAGTGATTTCCTGTTTGAAAAGAGTGCTAACTACTTCCACTCAGAAGAGGCTCC
  CAAGAGAGCCGCGTCTCTTGTTCCCAAGGCCAAGATCATCACTATCCTCATAGACCCCTCAGACAGGGCG
  TATTCCTGGTACCAGCACCAACGATCTCATGAAGATCCGGCTGCCCTGAGGTTCAATTTCTATGAAGTTA
  TCACGACAGGACACTGGGCCCCTCCTGATTTAAAAACATTGCAGAGGCGATGTCTGGTGCCTGGGTGGTA
  TGCAGTACATATAGAAAGATGGCTGGCCTACTTTTCTACTTCCCAGTTGCTAATTATTGATGGACAACAG
  CTGAGATCTGACCCAGCAACGGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTTACACCTCATTATAATT
  ACTCTGAGGCACTGACGTTTGATCCTCAAAAAGGCTTTTGGTGTCAACTACTGGAAGGAGGGAAAACCAA
  ATGTCTCGGGAAGAGCAAAGGACGGAGGTACCCACCCATGGATCCAGAGTCCAGAACTTTCCTCTCCAGT
  TACTACCGCGACCACAACGTGGAGCTCTCGAAGCTGCTGCACAGGTTGGGGCAGCCTCTGCCCTCGTGGC
  TAAGACAGGAGCTGCAGAAAGTGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217283 representing NM_001191849
  Red=Cloning site Green=Tags(s)
  
  MNLIVKFRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLVETTAEAECADIKNLPYRSIELKTIKP
  IDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYQMVIAPGKGDIPPLTDSGKGKYTLIIYENILKYV
  SMDSWNRELLEKYCIEYSVSIIGFHKANENSLPTTQLKGFPLNLFNNVALKDCSVNPQSPLLHITKGPKV
  EKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSFLSSQTLYATIIQDLGLHDGIQRVLFGNNLNFWLH
  KLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSG
  KFYHTGTEEEDAGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINLGYAVA
  PHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNNIMVLPRQTCGLFTHTIFYKEYPG
  GPQELDKSIKGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLANFVHSWTNLKLQTLPPVQLAHKY
  FELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIVSNLPSPKTF
  EEVQFFNGNNYHKGIEWYMDFFPTPSNITSDFLFEKSANYFHSEEAPKRAASLVPKAKIITILIDPSDRA
  YSWYQHQRSHEDPAALRFNFYEVITTGHWAPPDLKTLQRRCLVPGWYAVHIERWLAYFSTSQLLIIDGQQ
  LRSDPATVMDEVQKFLGVTPHYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRRYPPMDPESRTFLSS
  YYRDHNVELSKLLHRLGQPLPSWLRQELQKVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191849
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191849.1, NP_001178778.1
• RefSeq Size: 2619 bp
• RefSeq ORF: 2619 bp
• Locus ID: 362035
• Cytogenetics: 2q42
• MW: 100.6 kDa