Sema3g (NM_001100882) Rat Tagged ORF Clone
CAT#: RR217203
- TrueORF®
Sema3g (myc-DDK-tagged) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3G (Sema3g)
"NM_001100882" in other vectors (1)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Sema3g |
Synonyms | CSG53; RGD1306079 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217203 representing NM_001100882
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCTGCTCCGCTTGGGCCATCTGCTGCCTCCTGGGGAGCCTTCTGTTCCACATGGGCAGCCCGA ACCCTGGTCCCAGCACCAGTGTGCCTCGTCTGCGGCTCTCCTACAGAGATCTCCTGACTACCAACCGCTC TGCCATCTTTCTGGGTCCACGGGGCTCCCTAGACCTCCAAGTCATGTACCTGGATGAGTATCGGGACCGC CTCTTTCTGGGAGGCCGAGATGCCCTTTACTCTCTGAGACTGGATCAGGCATGGCCAGATCCCCGGGAGG TCTTGTGGCCGCCGCAGCCCGGACAGAAGGTGGAATGTGTCCGAAAGGGAAAAGACCCTTTGACCGAGTG TGCCAACTTCGTGCGGGTCTTACAACCCCACAACCGGACCCATCTGCTGGCCTGCGGCACTGGCGCCTTC CAGCCCATCTGCACCTTCATCACAGTGGGGCATCGGGGGGAGCATGTGCTCCACTTGGATCCTAGCAGCA TCGAAAATGGAAGGGGGCGTTGCCCACACGAGCCCAGCCGTCCCTTCGCCAGTACCTTTGTAGGTGGGGA GCTGTACACGGGCCTCACTGCTGATTTCCTCGGACGTGAGGCCATGATTTTCCGGAGTGGGGGTCCCCGA CCAGCCCTGCGTTCTGACTCTGACCAGAGCCTCCTACATGATCCCCGGTTTGTGATGGCTGCTCGGATCC CAGATAACTCCGACCGGGATGATGACAAAGTGTACTTCTTCTTCTCTGAGACTGTCCCTTCACCAGATGG TGGCCCAGGTCATGTCACCGTCAGCCGCGTGGGTCGAGTCTGTGTGAATGATGCTGGTGGCCAGCGGGTG CTGGTGAATAAATGGAGCACGTTCCTCAAGGCCAGGCTGGTATGCTCTGTGCCTGGCCCTGGCGGGGCTG AGACTCACTTTGACCAGCTGGAGGATGTGTTCCTGCTGTGGCCAAAGGCAGGGAAGAACCTCGAGGTGTA TGCGCTGTTCAGCACTGTCAGTGCTGTGTTCCGGGGCTTTGCTGTCTGTGTGTACCACATGGTAGACATC TGGGAGGTCTTCAACGGGCCCTTCGCTCACCGAGATGGCCCTCAACATCAGTGGGGACCCTATGGGGGCA AGGTGCCCTTCCCCCGGCCTGGTGTGTGTCCCAGCAAGATGACCGCACAGCCCGGCCGACCCTTTGGCAG CACCAAGGACTACCCGGATGAGGTGCTGCAGTTTGTCCGAGGCCACCCGCTCATGTTCCAGCCTGTGAGG CCTCGGCGTGGCCGCCCCGTCCTGGTCAAAACTCACCTGGCTCAGCAACTGTGCCAGATCGTGGTGGACA GAGTGGAGGCTGAGGATGGGACCTACGATGTCATCTTCCTAGGGACTGATTCAGGTTCTGTGCTCAAAAT CATCGCTCTCCAGGGTAGTGGCTTACCCGAACCGGAAGAGGTGGTTCTGGAGGAGCTCCAGGTGTTTAAG GTACCAACACCCATCACTGAGATGGAGATCTCTGTCAAAAGGCAAACGCTGTATGTGGGCTCTCCACTGG GCGTGGCCCGGCTGCAGCTGCACCAGTGTGAGACTTATGGCAGTGCCTGCGCTGAGTGCTGCCTGGCCCG GGACCCATACTGTGCTTGGGACGGCACTTCCTGTGCCCGCTACCGCCCCAGCTCGGGCAAGCGCAGGTTC CGACGACAGGACATCCGACATGGCAATCCTGCTGTGCAGTGTCTGGGCCAGGGCCAGAGCCAAGACAAAG CCTCGGGACTGGTGACCAGAGTCTTTGGCACAGAGCACAACAGTACCTTCCTTGAGTGCCGGCCCAAGTC TCCCCAGGCTGCAGTGCGCTGGTTCTTACAAAGGCCAGGGGATAAGGGGGCTGACCAGGTGAAGACAGAT GAGCGAGTTGTGCAGACGGACCAGGGGCTTCTGTTCCGAAGGCTCAGCCGCCTTGATGCAGGGAACTACA CCTGTACCACTCTGGAGCACGGCTTCTCCCAGACCGTGGTTCGTTTTGCCCTGGAGGTGATTGCGGCTGT GCAGCTGGACAGCCTGTTCCTTCGGGAGCCAAGGCTGGAGGAGCCCTCAGCCTGGCGAAGTGTGGCTTCT GCCTCACCCAAGACTTGGTATAAGGACATCCTTCAGCTCACTGGTTTCGCCAACCTGCCCCGCGTGGATG AGTACTGCGAACGTGTGTGGTGCAGGGGTGTCGGGGAGCGCTCGGGCTCCTTCCGCGGAAAGGGAAAGCA GGCGAAGGGCAAGAGCTGGGCGGGGCTAGAGCTGGGCAGGAAGATGAAGAGCCGGGTGCAGGCTGAGCAC AATCGGACCCCCCGGGAGGTAGAAGCCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217203 representing NM_001100882
Red=Cloning site Green=Tags(s) MARCSAWAICCLLGSLLFHMGSPNPGPSTSVPRLRLSYRDLLTTNRSAIFLGPRGSLDLQVMYLDEYRDR LFLGGRDALYSLRLDQAWPDPREVLWPPQPGQKVECVRKGKDPLTECANFVRVLQPHNRTHLLACGTGAF QPICTFITVGHRGEHVLHLDPSSIENGRGRCPHEPSRPFASTFVGGELYTGLTADFLGREAMIFRSGGPR PALRSDSDQSLLHDPRFVMAARIPDNSDRDDDKVYFFFSETVPSPDGGPGHVTVSRVGRVCVNDAGGQRV LVNKWSTFLKARLVCSVPGPGGAETHFDQLEDVFLLWPKAGKNLEVYALFSTVSAVFRGFAVCVYHMVDI WEVFNGPFAHRDGPQHQWGPYGGKVPFPRPGVCPSKMTAQPGRPFGSTKDYPDEVLQFVRGHPLMFQPVR PRRGRPVLVKTHLAQQLCQIVVDRVEAEDGTYDVIFLGTDSGSVLKIIALQGSGLPEPEEVVLEELQVFK VPTPITEMEISVKRQTLYVGSPLGVARLQLHQCETYGSACAECCLARDPYCAWDGTSCARYRPSSGKRRF RRQDIRHGNPAVQCLGQGQSQDKASGLVTRVFGTEHNSTFLECRPKSPQAAVRWFLQRPGDKGADQVKTD ERVVQTDQGLLFRRLSRLDAGNYTCTTLEHGFSQTVVRFALEVIAAVQLDSLFLREPRLEEPSAWRSVAS ASPKTWYKDILQLTGFANLPRVDEYCERVWCRGVGERSGSFRGKGKQAKGKSWAGLELGRKMKSRVQAEH NRTPREVEAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001100882 |
ORF Size | 2340 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001100882.1, NP_001094352.1 |
RefSeq Size | 3626 bp |
RefSeq ORF | 2343 bp |
Locus ID | 290562 |
Cytogenetics | 16p16 |
MW | 86.9 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217203 | Sema3g (untagged) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3G (Sema3g) |
USD 787.00 |
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