Elf4 (NM_001191735) Rat Tagged ORF Clone

CAT#: RR217068

Elf4 (myc-DDK-tagged) - Rat E74-like factor 4 (ets domain transcription factor) (Elf4)

Product Images

Circular map for RR217068

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elf4
• Synonyms: RGD1560743
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217068 representing NM_001191735
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATTGCCCTGCAGCCCAGCGATCTGGTCTTTGAGTTCGCAAGCAACGGGATGGATGATATCCACC
  AGCTGGAAGACCCTTCAGTGTTCCCAGCTGTGATCGTGGAACAGGTCCCCTATCCTGAATTAGTACATCT
  GTACTCAGGACTGGATCTGGATGAAGTCCACAGTGGCATCATAAGGGACAGGACCTTGTGCATGACACAG
  GATCAGATCTTAGAAGGCAGTATTTTGCTGACAGATGACAATGTGTCCACCTCAAATAACTCGTCATCTA
  CTGAAGTCCTATTCAATGTGGCAACTCCCAGTGATGTCCTGGATGAGAAGCAGATCTTCAGCACACCGGA
  AGTGCTTCCAGACTCCAACTCTGTTCAGGCCATCAATCTGCCCAACTTCCTGCTTTCCACCCCTGAACCT
  GATGACCCAAAGAAGACAAGTGATGCTGGTGACCAGAAGGAGTATTCTCTGGAGGAGGTTTCCAGAGAGG
  AAAATCTTAGGAAGATTGGGAAGTCACGGAAGAGAAACCGAAAGACAAAGAACAACCGAAGTACCTCACC
  TGTCACTGACCCCAGCGTGCCCATCCGGAAGAAATCCAAGGATGGCAAAGGCAGCACCATTTATCTGTGG
  GAGTTCCTCCTGGCTCTTCTGCAAGATAGAAACACCTGCCCCAAATACATCAAGTGGACGCAGCGAGAGA
  AAGGCATCTTCAAGTTGGTGGACTCCAAAGCTGTGTCCAAGCTGTGGGGCAAGCAGAAAAACAAACCTGA
  TATGAACTATGAGACTATGGGGCGGGCCCTAAGATATTACTATCAGAGAGGAATCCTGGCCAAAGTGGAA
  GGGCAGAGGCTGGTATACCAGTTCAAGGAGATGCCTAAGGACTTGGTGGTGATTGACGATGAGGAGGAGA
  GTACTGAAACACCAGAAGACTCTTCCCAGGCCTCTACTTCCTCCACACCCTCTACCAGTACCACCCGGAG
  ACCCAGCTCCAGGGTTGTAACCAGAGCCTCCCCCGAGGACAAGGATAGTCCTTCCTGGGAGAAGCCAAAG
  GTTGAACACACTGGTCTCCAGCCCTCTGCAAGCCTGGAGTTAGGATTATCTGTGGATGAGGAGGTCCCGA
  CTACCTCCACCATGATTGTCAGTCCACTACAGAGCCAGGCCAAGCCCGCCAAAACTGTGAGTACTTCAGC
  GCCCAGTAACATCCACCTAGGAGTAGCCCCAGTGGGGCCCGGCTCCACGGTTACCCTGCAGACCATCCCA
  CTGACTACGGTGCTGACCAATGGGCCTCCTGCCGGTACTACTGCTCCTGCTCAGCTCGTTCTTCAGAGTG
  TCCCACAGGTGTCAACGTTCAAGGACACCCTCACTTTGCAGACTTCCTTTCCCCTGAATGCCAGTTTGCA
  AGAGAACCAGGTGGCAACCCAAGGGACCCCATTGATCCTCAGTGGTCTCCCCCAACTTCTGGCCGGGGCC
  AACCATCAGAGCAACCCGGTAACATCCCAGGTCATAGGTGCTGGGTCAGCAGGACCCAGCTCTCAGCCCC
  CTGGGACTGTCATTGCTGCCTTTATCAGGACTTCTAATGGCACTTCAGTGCCTGTGGTTAAGGAGGGGCC
  CTTGAGGTCCTCTTCATATGTGCAGGGTGTGGTAACTGGGGCCCCAGTGGAGGGGCTGTTGGTCCCTGAA
  GAGACCCTAAGAGAGCTCCTGAGAGATCAGGCTCATCTTCAGCCACTTCAGAGTCAGGCGCTCTCAAGAG
  GTTCCCATAACCTGAGCCTTGTGGGGAACCAGACTTTATCTCCCCCTAGCCACCCCACTGTTGGGCTAAC
  TCCGGTGGCTGAACTTGAGCTCTCATCCAGCTCAGGGTCCCTGTTGGTAACTGAGCCTAATATGACCACA
  TCTGGGAGCCTGCTGACCAGATCCCCCACCCAGGCCCCTTTCTCCCCATTCAATCCCACTTCCCTCATTA
  AGATGGAGCCCCATGACATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217068 representing NM_001191735
  Red=Cloning site Green=Tags(s)
  
  MAIALQPSDLVFEFASNGMDDIHQLEDPSVFPAVIVEQVPYPELVHLYSGLDLDEVHSGIIRDRTLCMTQ
  DQILEGSILLTDDNVSTSNNSSSTEVLFNVATPSDVLDEKQIFSTPEVLPDSNSVQAINLPNFLLSTPEP
  DDPKKTSDAGDQKEYSLEEVSREENLRKIGKSRKRNRKTKNNRSTSPVTDPSVPIRKKSKDGKGSTIYLW
  EFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGILAKVE
  GQRLVYQFKEMPKDLVVIDDEEESTETPEDSSQASTSSTPSTSTTRRPSSRVVTRASPEDKDSPSWEKPK
  VEHTGLQPSASLELGLSVDEEVPTTSTMIVSPLQSQAKPAKTVSTSAPSNIHLGVAPVGPGSTVTLQTIP
  LTTVLTNGPPAGTTAPAQLVLQSVPQVSTFKDTLTLQTSFPLNASLQENQVATQGTPLILSGLPQLLAGA
  NHQSNPVTSQVIGAGSAGPSSQPPGTVIAAFIRTSNGTSVPVVKEGPLRSSSYVQGVVTGAPVEGLLVPE
  ETLRELLRDQAHLQPLQSQALSRGSHNLSLVGNQTLSPPSHPTVGLTPVAELELSSSSGSLLVTEPNMTT
  SGSLLTRSPTQAPFSPFNPTSLIKMEPHDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191735
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191735.1, NP_001178664.1
• RefSeq Size: 2448 bp
• RefSeq ORF: 1983 bp
• Locus ID: 302811
• Cytogenetics: Xq36
• MW: 71.3 kDa