Fpgs (NM_001146125) Rat Tagged ORF Clone
CAT#: RR216958
- TrueORF®
Fpgs (myc-DDK-tagged) - Rat folylpolyglutamate synthase (Fpgs)
"NM_001146125" in other vectors (1)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Fpgs |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216958 representing NM_001146125
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTGGGCGCGGAGCCACCTGTGCTCGGCTCTATCCTTGGCAGCTGTTTCTGCGCGTGGTGCAACGA TGGAGGGCGCGGCGTATCGGTGGTTGAGCGCGTGGCCAGCGCCGCAGGAGCCGAGCATGGAGTATCAGGA TGCTGTGCGCACGCTCAACACCCTGCAAACCAATGCCAGCTGCCTGGAGGAGGTAAAGCGTCAACGGAGT GACCCTCAGGCGCAGCTAGAAGCTATGGAGGTGTACCTCGCACGGAGTGGACTGCAGGTGGAGGATTTGA ACCAACTAAAAATTATCCATGTCACTGGGACCAAAGGAAAGGGCTCCACCTGTGCCTTCACTGAACGGAT CCTGCGGAATTACGGCCTGAAAACTGGCTTCTTTAGCTCTCCCCACCTGGTGCAGGTGCGTGAGCGGATT CGAATCAACGGGAAGCCCATCAGCCCCGAACTCTTTACCAAGTACTTCTGGCACCTCTATCAGCAGCTGG AGGAATTCAAGGATGACAGCCACATTTCCATGCCCTCTTACTTCCGCTTCCTCACACTCATGGCTTTCCA TGTCTTCCTCCAAGAGAAGGTGGACCTGGCCGTGGTGGAAGTGGGCATTGGTGGGGCTTTTGACTGCACC AACATCATCAGAAAGCCAGTGGTGTGTGGAGTCTCCTCTCTTGGCATTGACCACACCAGTCTGCTAGGAG ATACAGTGGAGAAAATAGCATGGCAGAAAGGGGGCATCTTTAAGCCTGGTGTCCCTGCCTTCACTGTGCT ACAGCCAGAAGGTCCCCTGGCCGTGCTGAGGGATAGAGCCCAGCAGATCTCATGCCCCCTGTACCTGTGT CCACCATTGGAAGCCCTGGAGCAGGTTGGATTGCCATTGAGCCTGGGCCTGGAGGGAGAGCACCAGCGGC ATAATGCAGCCTTGGCCTTGCAGCTGGCCCGTTGTTGGCTGGAGCAGCAGAACTACCATGACATCCAGGA GCTGAAGGCATCCAAGCCAAGCATTCGGTGGCAGCTGCCGCTGGCACCTGTGTTCCGCCCCACGCCCCAC ATGAGACGTGGGCTTCGGGACACAGAGTGGCCTGGTCGGACACAGGTACTCCAGCGGGGACCTGTTACCT GGTACCTGGATGGCGCTCATACCACCAGCAGTGTGCAGGCCTGTGTGCACTGGTACTGCCAGTCACTGGA GCGCAGCAGACGCACCGACGGTGGGCATGAAGTACACATCTTGCTCTTCAACTCCACTGGTGACAGGGAC TCTGCTGCCCTACTGAAGTTGCTGCAGCCCTGCCATTTTGATTACGCTGTCTTCTGCCCCAATGTGACAG AGATTTCATCCATAGGAAACGCAGACCAGCAGAACTTCACGGTGACTCTGGACCAAGTGCTCCTCCGCTG CCTGCAGCACCAGAAGCATTGGAACTGCCTGGCAGAGAAACAGGCCAGCTCCAACCTCTGGGGCAGCTGT GGCCCAGAGTCTGCCCGGCCAGGCTCCCTGCTGCTGGCACCGCACCCACCTCAGCCCACTAGCACGAGCT CCCTTGTTTTCAGCTGCATCTCCCACGCCTTGCAGTGGATCAGCCAAGGCCGGGATCCCATCTTTCAGCC CGAGAGCCCTCCAAGGAACCTTCTGAACCACCCCACGGCCAACAGCGGGGCGAGCATTCTCCGAGAGGCC GCTGCCATCCACGTACTGGTCACGGGGAGCCTGCACCTGGTGGGTGGGGCGCTGAGGCTGCTTGATCCCT CCATGTCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216958 representing NM_001146125
Red=Cloning site Green=Tags(s) MSWARSHLCSALSLAAVSARGATMEGAAYRWLSAWPAPQEPSMEYQDAVRTLNTLQTNASCLEEVKRQRS DPQAQLEAMEVYLARSGLQVEDLNQLKIIHVTGTKGKGSTCAFTERILRNYGLKTGFFSSPHLVQVRERI RINGKPISPELFTKYFWHLYQQLEEFKDDSHISMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCT NIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVLQPEGPLAVLRDRAQQISCPLYLC PPLEALEQVGLPLSLGLEGEHQRHNAALALQLARCWLEQQNYHDIQELKASKPSIRWQLPLAPVFRPTPH MRRGLRDTEWPGRTQVLQRGPVTWYLDGAHTTSSVQACVHWYCQSLERSRRTDGGHEVHILLFNSTGDRD SAALLKLLQPCHFDYAVFCPNVTEISSIGNADQQNFTVTLDQVLLRCLQHQKHWNCLAEKQASSNLWGSC GPESARPGSLLLAPHPPQPTSTSSLVFSCISHALQWISQGRDPIFQPESPPRNLLNHPTANSGASILREA AAIHVLVTGSLHLVGGALRLLDPSMSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146125 |
ORF Size | 1761 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146125.2, NP_001139597.2 |
RefSeq Size | 2586 bp |
RefSeq ORF | 1764 bp |
Locus ID | 687266 |
Cytogenetics | 3p11 |
MW | 65.3 kDa |
Gene Summary | human homolog catalyzes an ATP-dependent ligation step in the synthesis of poly(gamma-glutamate) folate metabolites [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216958 | Fpgs (untagged) - Rat folylpolyglutamate synthase (Fpgs) |
USD 602.00 |
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