Uap1 (NM_001191930) Rat Tagged ORF Clone

CAT#: RR216834

Uap1 (myc-DDK-tagged) - Rat UDP-N-acteylglucosamine pyrophosphorylase 1 (Uap1)

Product Images

Circular map for RR216834

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uap1
• Synonyms: RGD1561967
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216834 representing NM_001191930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTTAATGACCTCAGACAGAGGTTGTCCGCGGCTGGGCAGCAGCACCTGCTGCAGTTCTGGAGTG
  AGCTGTCAGAAGCCCAGCAGGGAGAACTTTATATGGAACTCCAAGCCATGAACTTTGAAGAGCTGAACTC
  ATTTTTCCAGAAGGCCGTTGGCGAATTTGATCGATCTTCTCACCAAGAAAAGGTGGATGCACGGATGGAG
  CCTGTGCCCAGACAGGTTTTGGGCAGTGCTACCAGAGATCAAGACCAGCTGCAGGCCTGGGAGAGCGAAG
  GACTTTCCCAGATTTCTCAGAACAAAGTAGCAGTTCTTCTTCTAGCTGGTGGGCAGGGGACAAGACTTGG
  AGTCACGTATCCCAAGGGCATGTATGATGTGGGCTTGCCATCCCACAAGACACTTTTCCAGATACAAGCA
  GAACGTATTCTGAAGCTGCAGCAGTTAGCTGAGAAACAGTATGGCAACAAGTGCACCATCCCCTGGTATA
  TAATGACCAGTGGCAGAACGATGGAATCGACAAAGGAGTTCTTTACAAAGCACAAGTTCTTTGGTTTAAA
  AAAGGAGAACGTGGTCTTTTTCCAGCAGGGAATGCTGCCTGCTATGAGCTTTGATGGGAAAGTTATCTTG
  GAAGAGAAAAACAAAGTCTCTATGGCTCCAGATGGGAATGGTGGTCTCTACCGGGCTCTTGCAGCCCAAA
  ACATTGTGGAGGACATGGAGCAAAGAGGCATCTGGAGTATTCATGTCTATTGTGTGGATAACATACTGGT
  GAAAGTGGCAGACCCTCGGTTCATTGGATTCTGCATTCAGAAAGGAGCAGACTGTGGGGCAAAGGTGGTA
  GAGAAAACCAACCCCACGGAGCCAGTCGGCGTGGTTTGCCGGGTGGATGGGGTGTACCAGGTGGTGGAGT
  ACAGTGAGATCTCTCTGGCCACAGCTCAGAAGCGGAGCTCAGACGGACGGCTGCTGTTCAACGCGGGGAA
  CATCGCCAATCACTTCTTCACTGTGCCGTTCCTGAAAGATGTTGTCAATGTTTACGAACCTCAGTTGCAG
  CACCATGTGGCTCAGAAGAAGATTCCATACGTGGATTCCCAGGGCCACTTAATTAAGCCAGACAAGCCAA
  ATGGAATAAAGATGGAAAAATTTGTCTTTGACATCTTCCAGTTTGCAAAGAAGTTTGTGGTGTACGAAGT
  ATTACGGGAAGACGAATTCTCCCCGCTCAAAAATGCAGACAGTCAGAATGGGAAGGACAACCCGACCACT
  GCAAGGCATGCCTTGATGTCCCTTCACCATTGCTGGGTCCTCAATGCTGGGGGACACTTCATAGATGAAA
  ATGGCTCCCGCCTCCCAGCCATCCCCCGTGCTACAAATGGAAAGTCAGAGACCATCACAGCTGATGTCAA
  TCACAACTTGAAGGATGCCAATGATGTGCCGATCCAGTGTGAGATCTCTCCCCTCATCTCTTACGCTGGA
  GAAGGACTTGAAGGCTATGTGGCAGATAAAGACTTCCATGCACCCTTAATCATCGATGAGAACGGAGTTC
  ATGAGCTGGTGAAAAATGGCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216834 representing NM_001191930
  Red=Cloning site Green=Tags(s)
  
  MNVNDLRQRLSAAGQQHLLQFWSELSEAQQGELYMELQAMNFEELNSFFQKAVGEFDRSSHQEKVDARME
  PVPRQVLGSATRDQDQLQAWESEGLSQISQNKVAVLLLAGGQGTRLGVTYPKGMYDVGLPSHKTLFQIQA
  ERILKLQQLAEKQYGNKCTIPWYIMTSGRTMESTKEFFTKHKFFGLKKENVVFFQQGMLPAMSFDGKVIL
  EEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVV
  EKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLKDVVNVYEPQLQ
  HHVAQKKIPYVDSQGHLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTT
  ARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRATNGKSETITADVNHNLKDANDVPIQCEISPLISYAG
  EGLEGYVADKDFHAPLIIDENGVHELVKNGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191930
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191930.1, NP_001178859.1
• RefSeq Size: 2249 bp
• RefSeq ORF: 1566 bp
• Locus ID: 498272
• Cytogenetics: 13q24
• MW: 58.4 kDa