Ppme1 (NM_001191838) Rat Tagged ORF Clone

CAT#: RR216537

Ppme1 (myc-DDK-tagged) - Rat protein phosphatase methylesterase 1 (Ppme1)

Product Images

Circular map for RR216537

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppme1
• Synonyms: PME-1; RGD1309683
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216537 representing NM_001191838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCCTTGAAAAAAGCATGCACCTCGGCCGCTTACCTTCTCGCCCTCCTCTACCCGGCAGCGGGG
  GCAGTCAGAGCGGAGCCAAGATGCGGATGGGCCCTGGAAGGAAGCGGGACTTTACCCCTGTTCCATGGAG
  TCAGTACTTCGAGTCAATGGAAGATGTGGAGGTGGAGAATGAAACTGGCAAGGATACTTTTCGAGTTTAC
  AAGAGTGGTTCCGAGGGTCCAGTCCTGCTGCTGCTTCATGGAGGAGGCCATTCTGCCCTTTCCTGGGCAG
  TATTCACGGCAGCAATCATCAGTAGAGTACAGTGTCGGATTGTGGCTCTGGATCTACGAAGCCATGGAGA
  AACTAAAGTCAAGAATTCTGAAGACCTGTCAGCAGAAACAATGGCAAAAGATGTTGGTAATGTGGTGGAA
  GCCATGTATGGCGACCTCCCTCCTCCCGTCATGCTGATTGGGCACAGCATGGGCGGTGCCATTGCAGTAC
  ACACAGCAGCAGCTAACCTGGTGCCAAGCCTTTTGGGTCTTTGTATGATTGATGTTGTGGAAGGTACAGC
  CATGGATGCCCTTAATAGCATGCAGAATTTCTTACGGGGTCGGCCCAAAACCTTCAAGTCTCTGGAGAAT
  GCTATTGAATGGAGTGTGAAGAGTGGCCAGATTCGAAATCTAGAATCTGCCCGTGTATCCATGGTCGGCC
  AAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAAGTTCCAAATCCATAGTAGAAGGAATCATAGAGGA
  GGAGGAAGAAGATGAAGAAGGAAGTGAGTCAGTTAACAAGAGGAAAAAGGAAGACGACATGGAAACCAAG
  AAGGATCATCCATACACCTGGAGAATTGAGCTGGCAAAAACAGAGAAGTACTGGGACGGCTGGTTCCGAG
  GCTTATCCAATCTCTTTCTTAGCTGTCCTATTCCTAAACTGCTGCTCTTGGCAGGTGTTGATAGATTGGA
  TAAAGATCTGACCATAGGCCAGATGCAGGGGAAGTTCCAGATGCAGGTCTTACCCCAGTGTGGCCATGCA
  GTCCATGAGGATGCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGACACAGGTTTGCAG
  AGCCCATCGGAGGATTCCAGTGTGTGTTTCCTGGCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216537 representing NM_001191838
  Red=Cloning site Green=Tags(s)
  
  MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFTPVPWSQYFESMEDVEVENETGKDTFRVY
  KSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNSEDLSAETMAKDVGNVVE
  AMYGDLPPPVMLIGHSMGGAIAVHTAAANLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLEN
  AIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPESSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMETK
  KDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHA
  VHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191838
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191838.1, NP_001178767.1
• RefSeq Size: 2436 bp
• RefSeq ORF: 1161 bp
• Locus ID: 361613
• UniProt ID: Q4FZT2
• Cytogenetics: 1q32
• MW: 42.3 kDa
• Gene Summary: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme (By similarity).[UniProtKB/Swiss-Prot Function]