Cebpb (NM_001301720) Rat Tagged ORF Clone
CAT#: RR215898
- TrueORF®
Cebpb (myc-DDK-tagged) - Rat CCAAT/enhancer binding protein (C/EBP), beta (Cebpb), transcript variant 1
"NM_001301720" in other vectors (1)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cebpb |
Synonyms | Il6dbp; NF-IL6; TCF5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215898 representing NM_001301720
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGGCTTCCCGTTCGCCCTGCGCGCCTACCTGGGCTACCAGGCGACGCCGAGCGGCAGCAGCG GCAGCCTGTCCACGTCGTCGTCGTCCAGCCCGCCCGGGACGCCGAGCCCCGCCGACGCCAAGGCCGCGCC CGCCGCCTGCTTCGCGGGGCCGCCGGCCGCGCCCGCCAAGGCCAAGGCCAAGAAGGCGGTGGACAAGCTG AGCGACGAGTACAAGATGCGGCGCGAGCGCAACAACATCGCGGTGCGCAAGAGCCGCGACAAGGCCAAGA TGCGCAACCTGGAGACGCAGCACAAGGTGCTGGAGCTGACGGCGGAGAACGAGCGGCTGCAGAAGAAGGT GGAGCAGCTGTCGCGAGAGCTCAGCACGCTGCGGAACTTGTTCAAGCAGCTGCCCGAGCCGCTGCTGGCC TCGGCGGGTCACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215898 representing NM_001301720
Red=Cloning site Green=Tags(s) MAAGFPFALRAYLGYQATPSGSSGSLSTSSSSSPPGTPSPADAKAAPAACFAGPPAAPAKAKAKKAVDKL SDEYKMRRERNNIAVRKSRDKAKMRNLETQHKVLELTAENERLQKKVEQLSRELSTLRNLFKQLPEPLLA SAGHC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001301720 |
ORF Size | 435 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301720.1, NP_001288649.1 |
RefSeq Size | 1475 bp |
RefSeq ORF | 438 bp |
Locus ID | 24253 |
UniProt ID | P21272 |
Cytogenetics | 3q42 |
MW | 15.6 kDa |
Gene Summary | This intronless gene encodes a member of the transcription factor family whose members contain a basic leucine-zipper domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. The encoded protein plays important roles in several cellular processes and in various diseases, including regulating cell proliferation, differentiation, apoptosis and neuroinflammation, and being involved in brain injury and inflammatory progression. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with different cellular localizations and distinct biological functions. [provided by RefSeq, Sep 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215898 | Cebpb (untagged) - Rat CCAAT/enhancer binding protein (C/EBP), beta (Cebpb), transcript variant 1 |
USD 165.00 |
{0} Product Review(s)
Be the first one to submit a review