Col1a2 (NM_053356) Rat Tagged ORF Clone

CAT#: RR214908

Col1a2 (Myc-DDK-tagged ORF) - Rat collagen, type I, alpha 2 (Col1a2), (10 ug)

Product Images

Circular map for RR214908

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col1a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214908 representing NM_053356
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGCTTTGTGGATACGCGGACTCTGTTGCTGCTTGCAGTAACGTCGTGCCTAGCAACATGCCAAT
  CTTTACAAATGGGATCTGTACGGAAGGGCCCCACTGGAGACAGAGGACCGCGTGGACAAAGGGGCCCAGC
  AGGTCCCCGAGGCAGAGATGGTGTTGATGGTCCCGTTGGCCCTCCTGGTCCCCCTGGTGCCCCTGGCCCC
  CCTGGTCCCCCTGGCCCCCCTGGTCTTACTGGGAACTTTGCTGCTCAGTATTCTGACAAAGGAGTTTCAG
  CTGGCCCCGGACCAATGGGCTTAATGGGACCCAGAGGCCCTCCTGGTGCTGTCGGAGCCCCTGGCCCTCA
  AGGTTTCCAAGGACCTGCTGGTGAGCCTGGTGAGCCCGGACAAACGGGTCCTGCAGGTTCCCGTGGGCCA
  GCTGGCCCTCCTGGCAAGGCCGGTGAAGATGGTCACCCTGGAAAACCTGGAAGACCTGGCGAGAGAGGAG
  TCGTTGGGCCACAAGGTGCTCGTGGTTTCCCTGGGACTCCTGGACTTCCTGGCTTCAAAGGCATTCGAGG
  ACACAACGGTCTGGATGGATTGAAAGGACAGCCTGGTGCCCAGGGTGTGAAGGGTGAACCCGGTGCCCCT
  GGAGAGAATGGAACCCCAGGTCAAGCAGGAGCCCGAGGGCTTCCTGGTGAGAGAGGGCGTGTCGGAGCTC
  CTGGTCCAGCTGGTGCCCGTGGCAGTGATGGCAGTGTTGGCCCCGTCGGCCCTGCTGGTCCCATTGGGTC
  TGCTGGTCCTCCTGGTTTCCCAGGTGCTCCTGGTCCCAAGGGTGAACTTGGGCCTGTTGGTAACCCTGGC
  CCTGCTGGTCCTGCTGGTCCCCGTGGTGAAGCGGGTCTTCCGGGTCTTTCTGGCCCCGTTGGACCTCCTG
  GCAACCCTGGAGCAAATGGCCTCACTGGTGCCAAGGGAGCCACTGGACTCCCTGGTGTTGCTGGGGCTCC
  TGGTCTCCCCGGTCCCCGTGGTATTCCTGGCCCCGTTGGTGCTGCTGGTGCCACTGGTCCCAGAGGACTT
  GTTGGTGAACCTGGTCCTGCTGGCTCCAAAGGAGAGACTGGTAACAAGGGTGAGCCTGGCTCTGCTGGAG
  CCCAAGGTCCTCCTGGTCCCAGCGGTGAAGAAGGAAAGAGAGGGTCCCCTGGAGAGCCTGGATCTGCTGG
  TCCTGCAGGGCCTCCAGGGCTGAGAGGCAGCCCTGGTTCTCGAGGTCTTCCTGGAGCTGATGGCAGAGCT
  GGTGTGATGGGCCCTCCTGGCAATCGTGGTTCAACTGGCCCTGCTGGAGTCCGAGGTCCTAATGGAGATG
  CCGGTCGCCCTGGAGAACCTGGTCTCATGGGACCCAGAGGTCTTCCCGGGTCTCCTGGAAATGTTGGCCC
  AGCTGGTAAAGAAGGCCCTGTGGGTCTCCCTGGCATCGATGGCAGACCTGGCCCAATTGGCCCAGCTGGA
  CCAAGAGGTGAAGCTGGCAACATCGGATTCCCTGGACCCAAAGGCCCCTCTGGTGATCCTGGCAAACCTG
  GTGAGAAAGGCCATCCCGGTCTCGCTGGTGCTCGGGGAGCTCCAGGTCCCGACGGCAACAATGGTGCTCA
  GGGCCCTCCTGGACCTCAGGGTGTTCAAGGTGGCAAAGGTGAACAGGGCCCTGCTGGTCCTCCAGGCTTC
  CAGGGTCTCCCGGGTCCCTCTGGTACCGCTGGAGAAGTTGGCAAGCCGGGAGAAAGGGGTCTTCCCGGTG
  AATTCGGTCTCCCTGGTCCTGCTGGTCCAAGAGGAGAACGTGGTCCCCCAGGTGAGAGTGGAGCTGCTGG
  TCCTTCTGGTCCTATTGGAATCCGAGGTCCCTCTGGAGCCCCAGGGCCTGATGGGAACAAGGGTGAAGCT
  GGTGCAGTCGGTGCTCCAGGCTCTGCTGGTGCCTCTGGTCCTGGTGGGCTTCCAGGAGAGAGGGGTGCTG
  CTGGCATACCTGGAGGCAAAGGAGAAAAGGGTGAAACTGGTCTCAGAGGTGAAATCGGCAACCCTGGTAG
  AGATGGTGCTCGAGGTGCTCCTGGTGCTATAGGTGCCCCTGGTCCCGCTGGAGCCTCAGGTGACCGGGGC
  GAAGCTGGTGCTGCTGGTCCTTCTGGCCCTGCTGGTCCTCGAGGTAGCCCTGGTGAACGTGGTGAGGTTG
  GTCCTGCTGGCCCCAATGGATTTGCTGGTCCTGCTGGTTCTGCTGGCCAACCTGGTGCTAAAGGAGAAAA
  GGGAACCAAAGGGCCTAAGGGAGAAAATGGCATCGTTGGTCCAACCGGACCTGTCGGAGCTGCTGGCCCA
  TCTGGTCCTAATGGCCCACCTGGCCCTGCTGGAAGTCGTGGTGATGGAGGGCCCCCTGGTATGACTGGCT
  TCCCTGGTGCTGCTGGACGGACTGGTCCCCCCGGACCCAGTGGTATTACTGGCCCCCCTGGTCCCCCTGG
  TGCTGCTGGTAAAGAAGGTATTCGTGGTCCCCGTGGTGACCAAGGTCCAGTTGGCCGAACTGGAGAAATA
  GGTGCATCTGGTCCCCCTGGATTTGCTGGCGAGAAGGGTCCTTCTGGAGAACCTGGTACCACAGGACCCC
  CTGGCACTGCAGGTCCTCAGGGTCTTCTTGGTGCTCCCGGTATTCTGGGTCTCCCTGGCTCTCGAGGTGA
  ACGTGGCCAGCCAGGTATTGCTGGTGCTCTGGGTGAACCTGGTCCTCTGGGCATTGCAGGCCCTCCTGGG
  GCCCGTGGTCCCCCTGGTGCTGTGGGCAGTCCTGGAGTTAATGGTGCCCCTGGTGAAGCCGGTCGTGATG
  GTAACCCTGGCAGTGATGGTCCCCCAGGTCGTGATGGTCAACCCGGACACAAGGGAGAGCGTGGTTACCC
  TGGCAACATTGGTCCCACTGGTGCTGCAGGTGCTCCTGGTCCTCATGGTTCTGTGGGTCCTGCTGGGAAA
  CATGGAAACCGTGGTGAGCCTGGTCCTGCTGGTTCTGTTGGTCCCGTTGGTGCTGTCGGTCCAAGAGGTC
  CTAGTGGCCCTCAAGGTATTCGAGGTGACAAGGGAGAGCCTGGTGACAAAGGGGCCAGAGGTCTTCCTGG
  CTTAAAAGGACACAATGGATTACAGGGTCTTCCTGGTCTTGCTGGGCTGCATGGTGACCAAGGAGCTCCT
  GGTCCCGTGGGTCCTGCTGGTCCCAGGGGTCCTGCTGGTCCTTCTGGCCCCATTGGCAAAGATGGTAGAT
  CTGGACATCCTGGACCAGTCGGTCCTGCTGGCGTTCGTGGCTCTCAGGGTAGCCAAGGTCCCGCTGGTCC
  CCCTGGTCCCCCTGGGCCTCCTGGGCCTCCTGGTGTCAGCGGAGGAGGCTATGACTTTGGTTTCGAAGGA
  GGCTTCTACAGGGCTGACCAGCCTCGCTCACAGCCTTCACTCAGACCCAAGGACTATGAAGTTGATGCAA
  CTCTGAAATCTCTCAATAACCAAATCGAGACCCTTCTCACTCCTGAAGGCTCTAGAAAGAACCCTGCCCG
  CACATGCCGTGACTTAAGACTCAGCCACCCAGAGTGGAAGAGCGATTACTACTGGATTGACCCTAACCAA
  GGATGCACTATGGATGCCATCAAAGTGTACTGCGATTTCTCTACTGGTGAAACCTGCATCCAGGCCCAAC
  CTGTCAACACCCCAGCCAAGAATGCATACAGCCGTGCCCAGGCCAACAAGCATGTCTGGTTAGGAGAGAC
  CATCAATGGTGGCAGCCAGTTTGAATACAACGCAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTC
  GCCTTCATGCGCCTGCTAGCCAACCGTGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGT
  ACCTGGACGAGGAGACAGGCCGCCTGAATAAGGCTGTCATTCTGCAGGGCTCCAACGACGTCGAACTTGT
  TGCTGAGGGCAACAGCAGATTCACCTACACTGTCCTTGTCGATGGCTGCTCCAAAAAGACAAATGAATGG
  GACAAGACAGTCATTGAATACAAAACGAATAAGCCATCTCGCCTGCCATTCCTTGACATTGCACCTCTGG
  ACATCGGTGGTACTAACCAAGAATTCCGTGTGGAGGTTGGCCCTGTCTGTTTCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214908 representing NM_053356
  Red=Cloning site Green=Tags(s)
  
  MLSFVDTRTLLLLAVTSCLATCQSLQMGSVRKGPTGDRGPRGQRGPAGPRGRDGVDGPVGPPGPPGAPGP
  PGPPGPPGLTGNFAAQYSDKGVSAGPGPMGLMGPRGPPGAVGAPGPQGFQGPAGEPGEPGQTGPAGSRGP
  AGPPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAQGVKGEPGAP
  GENGTPGQAGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPG
  PAGPAGPRGEAGLPGLSGPVGPPGNPGANGLTGAKGATGLPGVAGAPGLPGPRGIPGPVGAAGATGPRGL
  VGEPGPAGSKGETGNKGEPGSAGAQGPPGPSGEEGKRGSPGEPGSAGPAGPPGLRGSPGSRGLPGADGRA
  GVMGPPGNRGSTGPAGVRGPNGDAGRPGEPGLMGPRGLPGSPGNVGPAGKEGPVGLPGIDGRPGPIGPAG
  PRGEAGNIGFPGPKGPSGDPGKPGEKGHPGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPAGPPGF
  QGLPGPSGTAGEVGKPGERGLPGEFGLPGPAGPRGERGPPGESGAAGPSGPIGIRGPSGAPGPDGNKGEA
  GAVGAPGSAGASGPGGLPGERGAAGIPGGKGEKGETGLRGEIGNPGRDGARGAPGAIGAPGPAGASGDRG
  EAGAAGPSGPAGPRGSPGERGEVGPAGPNGFAGPAGSAGQPGAKGEKGTKGPKGENGIVGPTGPVGAAGP
  SGPNGPPGPAGSRGDGGPPGMTGFPGAAGRTGPPGPSGITGPPGPPGAAGKEGIRGPRGDQGPVGRTGEI
  GASGPPGFAGEKGPSGEPGTTGPPGTAGPQGLLGAPGILGLPGSRGERGQPGIAGALGEPGPLGIAGPPG
  ARGPPGAVGSPGVNGAPGEAGRDGNPGSDGPPGRDGQPGHKGERGYPGNIGPTGAAGAPGPHGSVGPAGK
  HGNRGEPGPAGSVGPVGAVGPRGPSGPQGIRGDKGEPGDKGARGLPGLKGHNGLQGLPGLAGLHGDQGAP
  GPVGPAGPRGPAGPSGPIGKDGRSGHPGPVGPAGVRGSQGSQGPAGPPGPPGPPGPPGVSGGGYDFGFEG
  GFYRADQPRSQPSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWKSDYYWIDPNQ
  GCTMDAIKVYCDFSTGETCIQAQPVNTPAKNAYSRAQANKHVWLGETINGGSQFEYNAEGVSSKEMATQL
  AFMRLLANRASQNITYHCKNSIAYLDEETGRLNKAVILQGSNDVELVAEGNSRFTYTVLVDGCSKKTNEW
  DKTVIEYKTNKPSRLPFLDIAPLDIGGTNQEFRVEVGPVCFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053356
• ORF Size: 4116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053356.1, NP_445808.1
• RefSeq Size: 4474 bp
• RefSeq ORF: 4119 bp
• Locus ID: 84352
• UniProt ID: P02466
• Cytogenetics: 4q21
• MW: 129.6 kDa
• Gene Summary: structural constituent of the extracellular matrix [RGD, Feb 2006]