Hspa4 (NM_153629) Rat Tagged ORF Clone
CAT#: RR213069
- TrueORF®
Hspa4 (Myc-DDK-tagged ORF) - Rat heat shock protein 4 (Hspa4), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_153629" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hspa4 |
Synonyms | Hsp70; Hsp110; irp94 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR213069 representing NM_153629
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGTGGGCATAGACCTGGGCTTTCAGAGCTGCTATGTCGCTGTGGCCCGCGCCGGCGGCATTG AGACGATCGCTAATGAGTATAGCGACCGCTGCACGCCGGCTTGTGTTTCTTTCGGTCCTAAGAATCGTTC AGTTGGAGCAGCAGCAAAGAGCCAGGTAATTTCCAATGCCAAGAACACAGTTCAAGGGTTTAAAAGATTT CATGGGCGTGCATTCTCAGATCCATTTGTGGAAGCAGAAAAATCTAACCTTGCATATGATATTGTACAGT TGCCCACTGGATTAACGGGCATAAAGGTTACATATATGGAAGAAGAGAGGAATTTTACCACTGAACAAGT GACTGCCATGCTCTTGTCAAAACTGAAGGAGACAGCAGAAAGTGTTCTTAAGAAGCCTGTTGTAGACTGT GTTGTTTCGGTTCCTAGTTTCTACACTGATGCAGAACGACGGTCAGTGATGGATGCCACACAGATTGCTG GTCTCAATTGCTTGCGGTTAATGAATGAAACAACTGCAGTTGCTCTTGCTTATGGAATCTATAAACAAGA CCTTCCTGCCTTAGAAGAAAAACCAAGAAATGTAGTTTTTGTAGATATGGGACATTCTGCCTATCAAGTT TCTGTATGTGCATTTAATAGAGGAAAACTGAAAGTTTTGGCCACAGCTTTTGATACAACATTGGGAGGCA GAAAATTTGATGAGGTGTTGGTAAATCACTTCTGTGAAGAATTTGGAAAGAAATACAAGCTAGACATAAA ATCTAAAGTTCGTGCTTTATTGCGACTCTCTCAAGAGTGTGAGAAACTCAAGAAATTAATGAGTGCGAAT GCTTCAGACCTCCCTTTGAGTATTGAATGCTTTATGAACGATATTGATGTATCGGGAACCATGAATAGAG GCAAATTTTTGGAGATGTGTGATGATCTCTTAGCAAGAGTGGAGCCGCCACTTCGTAGCATCTTGGACCA ATCCAAGTTAAAGAAAGAAGATATTTATGCAGTGGAGATAGTTGGTGGAGCCACACGGATCCCTGCCGTG AAAGAGAAGATCAGCAAGTTCTTTGGTAAAGAACTCAGCACAACATTAAATGCGGATGAAGCAGTCACTC GAGGCTGTGCTCTGCAGTGTGCAATCTTATCACCTGCTTTCAAAGTCAGAGAATTTTCTATCACTGATGT GGTACCATATCCAATATCTTTGAGGTGGAATTCTCCAGCTGAAGAGGGGTCAAGTGACTGTGAAGTCTTC CCCAAAAACCATGCTGCTCCTTTCTCCAAAGTCCTCACCTTTTATAGAAAGGAACCGTTCACTCTGGAGG CCTACTATAGTTCTCCTCAGGATTTGCCCTATCCAGATCCTGCTATAGCTCAGTTTTCAGTTCAGAAAGT CACTCCACAGTCTGATGGCTCCAGCTCAAAAGTGAAAGTCAAGGTCCGGGTAAATGTGCATGGCATTTTC AGTGTGTCCAGTGCGGCCTTAGTAGAGGTGCACAAGTCCGAGGAGAGCGAGGAGCCAATGGAGACTGATC AGAATGCAAAGGAAGAAGAGAAGATGCAGGTGGACCAGGAAGAACCACATACTGAAGAGCAACAGCCACA GACACCAGCAGAAAACAAGGCGGAGTCTGAGGAGATGGAGACCTCTCAAGCTGGATCAAAAGATAAAAAG ATGGACCAACCACCTCAAGCAAAGAAGGCAAAAGTGAAGACCAGCACCGTGGACCTGCCCATTGAGAGCC AGCTGCTGTGGCAGCTGGACCGGGAAATGCTCGGCCTGTACACAGAGAATGAGGGTAAGATGATCATGCA GGATAAACTGGAGAAGGAACGGAATGACGCCAAAAATGCGGTGGAAGAGTATGTGTATGAGATGAGAGAC AAACTTAGTGGTGAATATGAGAAGTTTGTGAGTGAAGATGATCGTAATAATTTTACTTTGAAATTAGAGG ATACAGAAAACTGGCTGTATGAAGATGGAGAAGATCAACCAAAGCAAGTTTATGTAGACAAATTGGCTGA GTTAAGAACTTTAGGTCAACCTATTAAGACACGGTTCCAGGAATCTGAAGAAAGACCAAAATTATTTGAA GAATTAGGGAAGCAAATCCAACAGTATATGAAAGTGATCAGCTCTTTCAAAAACAAGGAGGACCAGTATG AACACTTGGATGCTGCTGACATGACAAAGGTAGAGAAAAGCACCAATGAGGCGATGGAGTGGATGAATAG TAAGCTGAACCTGCAGAACAAACAGAGCCTGACGGCGGATCCAGTTGTCAAGACAAAAGAGATTGAAGCT AAAATTAAGGAGCTGACAAATATTTGCAGTCCTATAATTTCAAAGCCCAAACCCAAAGTGGAACCCCCTA AGGAGGAACCAAAACATGCTGAGCAGAATGGACCAGTGGATGGCCAAGGAGACAACCCAGGCACCCAAGC TGCTGAGCACGGTGCAGACACAGCTGTGCCTTCTGATGGTGACAAGAAGCTTCCTGAGATGGACATTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213069 representing NM_153629
Red=Cloning site Green=Tags(s) MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACVSFGPKNRSVGAAAKSQVISNAKNTVQGFKRF HGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDC VVSVPSFYTDAERRSVMDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQV SVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKVRALLRLSQECEKLKKLMSAN ASDLPLSIECFMNDIDVSGTMNRGKFLEMCDDLLARVEPPLRSILDQSKLKKEDIYAVEIVGGATRIPAV KEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVF PKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIF SVSSAALVEVHKSEESEEPMETDQNAKEEEKMQVDQEEPHTEEQQPQTPAENKAESEEMETSQAGSKDKK MDQPPQAKKAKVKTSTVDLPIESQLLWQLDREMLGLYTENEGKMIMQDKLEKERNDAKNAVEEYVYEMRD KLSGEYEKFVSEDDRNNFTLKLEDTENWLYEDGEDQPKQVYVDKLAELRTLGQPIKTRFQESEERPKLFE ELGKQIQQYMKVISSFKNKEDQYEHLDAADMTKVEKSTNEAMEWMNSKLNLQNKQSLTADPVVKTKEIEA KIKELTNICSPIISKPKPKVEPPKEEPKHAEQNGPVDGQGDNPGTQAAEHGADTAVPSDGDKKLPEMDID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153629 |
ORF Size | 2520 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153629.1, NP_705893.1 |
RefSeq Size | 4521 bp |
RefSeq ORF | 2523 bp |
Locus ID | 266759 |
UniProt ID | O88600 |
Cytogenetics | 10q22 |
MW | 94.1 kDa |
Gene Summary | heat shock protein; may have an important role following forebrain ischemia and may allow astrocytes to protect neurons [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN213069 | Hspa4 (untagged ORF) - Rat heat shock protein 4 (Hspa4), (10 ug) |
USD 847.00 |
|
RR213069L3 | Lenti ORF clone of Hspa4 (Myc-DDK-tagged ORF) - Rat heat shock protein 4 (Hspa4), (10 ug) |
USD 1,146.00 |
|
RR213069L4 | Lenti ORF clone of Hspa4 (mGFP-tagged ORF) - Rat heat shock protein 4 (Hspa4), (10 ug) |
USD 1,146.00 |
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