Clcn3 (NM_053363) Rat Tagged ORF Clone
CAT#: RR212863
- TrueORF®
Clcn3 (Myc-DDK-tagged ORF) - Rat chloride channel 3 (Clcn3), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_053363" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Clcn3 |
Synonyms | ClC-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212863 representing NM_053363
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATAGAAACAGCTACAACAGCATAACGAGCGCGAGTA GCGATGAGGAGCTCCTAGATGGAGCAGGTGCTATTATGGACTTTCAGACTTCCGAAGACGACAATTTGTT AGACGGTGACACAGCAGCTGGAACTCATTATACAATGACAAATGGAGGCAGCATTAACAGCTCCACACAC TTGCTGGATCTTTTGGATGAGCCCATCCCAGGTGTCGGTACATACGATGATTTCCATACTATTGACTGGG TGCGAGAAAAGTGTAAGGACAGAGAAAGGCACAGACGGATCAACAGTAAAAAAAAAGAATCAGCATGGGA AATGACAAAAAGTCTATATGATGCTTGGTCAGGATGGCTTGTAGTTACACTGACGGGATTGGCGTCAGGG GCGCTGGCTGGATTGATAGACATTGCTGCTGACTGGATGACTGACCTGAAAGAGGGCATCTGCCTCAGTG CATTGTGGTACAACCATGAACAGTGTTGTTGGGGCTCTAATGAGACGACGTTTGAAGAGAGGGATAAATG TCCACAGTGGAAAACATGGGCAGAGTTAATCATAGGTCAAGCAGAGGGTCCTGGATCTTATATCATGAAC TACATCATGTACATCTTTTGGGCTTTGAGTTTTGCCTTTCTTGCAGTTTCCTTGGTGAAAGTATTTGCTC CGTATGCCTGTGGCTCCGGAATTCCAGAGATTAAAACTATTTTGAGTGGATTCATCATCAGAGGTTACTT GGGAAAGTGGACTTTAATGATTAAAACCATCACATTAGTGCTGGCTGTGGCATCAGGTTTGAGTTTAGGA AAAGAAGGTCCCTTGGTACATGTTGCCTGCTGCTGTGGAAATATCTTTTCCTACCTCTTTCCAAAGTATA GCACAAATGAAGCTAAAAAGAGGGAGGTGCTGTCAGCCGCCTCAGCTGCTGGTGTCTCTGTGGCTTTTGG TGCGCCAATTGGAGGAGTGCTTTTTAGTTTGGAGGAGGTTAGCTATTATTTTCCTCTCAAAACTTTATGG AGATCGTTTTTTGCTGCTTTGGTGGCGGCCTTTGTTTTGAGATCCATCAATCCATTTGGTAACAGCCGTC TGGTCCTTTTTTATGTGGAGTATCATACACCATGGTACCTTTTTGAACTGTTTCCTTTTATTCTCCTAGG GGTATTTGGAGGGCTTTGGGGAGCTTTTTTTATTAGGGCAAATATTGCCTGGTGTCGCCGACGCAAGTCC ACCAAATTTGGAAAGTATCCTGTTCTTGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATAGCCTTCC CCAACCCATACACAAGGCTCAACACCAGTGAACTGATTAAAGAGCTGTTTACAGACTGTGGCCCCTTGGA ATCCTCCTCTCTTTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTTGATGATATTCCTGACCGA CCAGCAGGCGTTGGAGTATATTCAGCTATCTGGCAGTTGTGCCTAGCACTCATATTTAAAATAATAATGA CAGTATTCACTTTTGGTATCAAGGTCCCGTCGGGCCTGTTTATCCCCAGCATGGCGATCGGAGCCATTGC AGGGAGGATTGTGGGGATTGCTGTGGAGCAGCTCGCCTACTACCACCACGACTGGTTCATCTTCAAGGAG TGGTGTGAGGTCGGGGCTGACTGCATCACTCCTGGGCTGTATGCCATGGTTGGTGCTGCCGCATGCTTAG GTGGTGTGACAAGAATGACTGTCTCTCTGGTGGTTATTGTTTTTGAACTTACTGGAGGCTTGGAATATAT TGTTCCTCTTATGGCTGCAGTAATGACCAGTAAATGGGTTGGTGATGCCTTTGGTAGGGAAGGTATTTAT GAAGCACACATCCGACTAAATGGGTACCCTTTCTTGGATGCAAAAGAAGAATTCACTCATACAACCCTGG CTGCTGATGTCATGAGACCTCGAAGAAGTGACCCTCCCTTAGCTGTTTTGACACAGGACAATATGACAGT AGATGACATAGAAAACATGATTAATGAGACCAGCTATAATGGCTTTCCTGTTATAATGTCAAAAGAATCT CAGAGATTAGTGGGATTTGCCCTCAGAAGAGACCTGACAATTGCAATAGAAAGTGCCAGAAAAAAACAAG AAGGTGTTGTTGGCAGTTCTCGGGTGTGCTTTGCCCAACACACTCCATCTCTTCCAGCAGAAAGTCCTCG GCCATTAAAACTGAGAAGCATCCTTGACATGAGTCCTTTTACAGTGACAGACCACACCCCAATGGAGATT GTGGTAGACATCTTCCGAAAGCTTGGTCTGAGGCAGTGCCTTGTCACTCACAATGGGCGCCTCCTTGGCA TTATAACAAAAAAAGATATCCTCCGTCATATGGCCCAGACGGCAAACCAAGACCCCGCTTCAATAATGTT CAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212863 representing NM_053363
Red=Cloning site Green=Tags(s) MESEQLFHRGYYRNSYNSITSASSDEELLDGAGAIMDFQTSEDDNLLDGDTAAGTHYTMTNGGSINSSTH LLDLLDEPIPGVGTYDDFHTIDWVREKCKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASG ALAGLIDIAADWMTDLKEGICLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMN YIMYIFWALSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLG KEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVLSAASAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLW RSFFAALVAAFVLRSINPFGNSRLVLFYVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRKS TKFGKYPVLEVIIVAAITAVIAFPNPYTRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDR PAGVGVYSAIWQLCLALIFKIIMTVFTFGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKE WCEVGADCITPGLYAMVGAAACLGGVTRMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIY EAHIRLNGYPFLDAKEEFTHTTLAADVMRPRRSDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKES QRLVGFALRRDLTIAIESARKKQEGVVGSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEI VVDIFRKLGLRQCLVTHNGRLLGIITKKDILRHMAQTANQDPASIMFN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053363 |
ORF Size | 2454 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053363.2, NP_445815.2 |
RefSeq Size | 3826 bp |
RefSeq ORF | 2457 bp |
Locus ID | 84360 |
UniProt ID | P51792 |
Cytogenetics | 16p12 |
MW | 90.9 kDa |
Gene Summary | mediates transepithelial chloride transport [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212863 | Clcn3 (untagged ORF) - Rat chloride channel 3 (Clcn3), (10 ug) |
USD 825.00 |
|
RR212863L3 | Lenti ORF clone of Clcn3 (Myc-DDK-tagged ORF) - Rat chloride channel 3 (Clcn3), (10 ug) |
USD 1,124.00 |
|
RR212863L4 | Lenti ORF clone of Clcn3 (mGFP-tagged ORF) - Rat chloride channel 3 (Clcn3), (10 ug) |
USD 1,124.00 |
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