Nuak2 (NM_001007617) Rat Tagged ORF Clone

CAT#: RR212466

Nuak2 (Myc-DDK-tagged ORF) - Rat NUAK family, SNF1-like kinase, 2 (Nuak2), (10 ug)

Product Images

Circular map for RR212466

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nuak2
• Synonyms: 1200013b22rik; Snark; UV126
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212466 representing NM_001007617
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGGTGGCCTTACACCGGCGCGGGAACCTGGCTCCCTCGGCCTCCGCCCTGGCCACGGAGAGCG
  CCCGGCCGCTGGCGGACCGGCTCATCAAGTCGCCCAAACCTCTGATGAAGAAGCAGGCGGTGAAGCGGCA
  CCATCACAAACACAACCTGAGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAGGGCACCTACGGGAAG
  GTGAAGAAAGCACGAGAGAGCTCGGGACGCCTGGTGGCCATCAAGTCTATCAGGAAGGACAAAATCAAAG
  ATGAGCAGGATCTGTTGCACATAAGGAGGGAGATCGAGATCATGTCTTCACTCAACCACCCCCACATCAT
  TGCCATCCATGAAGTGTTTGAGAACAGCAGCAAGATTGTGATTGTCATGGAGTACGCCAGCCGAGGCGAT
  CTGTACGATTACATCAGTGAGCGGCCACGGCTGAATGAGCGGGACGCCAGGCATTTCTTCCGACAGATCG
  TGTCCGCCCTGCACTACTGCCACCAGAACGGGATTGTTCACCGGGACCTCAAGCTGGAGAACATCCTTCT
  AGATGCCAGTGGCAACATCAAGATTGCTGATTTTGGCCTCTCCAACCTGTATCACAAAGGCAAGTTCCTC
  CAGACGTTCTGTGGGAGCCCTCTCTATGCCTCACCTGAGATCGTCAACGGGAAGCCCTATGTGGGCCCAG
  AGGTGGACAGCTGGTCTCTGGGCGTTCTTCTGTACATCCTGGTGCATGGCACCATGCCCTTTGACGGGCA
  GGATCATAAAACCCTGGTGAAACAAATCAGTAGCGGGGCTTACCGAGAGCCGTGCAAACCGTCTGATGCC
  TGTGGCCTGATCCGGTGGCTGTTAATGGTGAATCCCATCCGTCGGGCCACTCTGGAGGATGTAGCCAGTC
  ATTGGTGGGTCAACTGGGGTTACAGCACCCGAATTGGGGAACAGGAAGCTCTGCGAGAGGGTGGGCACCC
  TAGCGGTGACTCTGGCCGGGCCTCTATGGCGGACTGGTTACGTCGCTCCTCCCGCCCCCTCCTGGAGAAT
  GGAGCCAAAGTGTGTAGCTTCTTCAAGCAGCATGTGCCGGGAGGTGGAAGCACGGGACCGGGGCTGGAGC
  GGCAACATTCTCTTAAGAAGTCCCGCAAGGAGAATGACATGGCTCAGACTCTGCAGAATGACCCAGCTGA
  AGATACTTCCTCTCGCCCTGGCAAGAACAGCCTCAAGCTTCCGAAAGGTATCCTCAAGAAAAAGGCCTCT
  CCCTCATCGGGGGAGGTACAGGAGGGCCCTCAGGAACTCAGACCAGTGTCCAATACCCCAGGGCAGCCTG
  TCCCTGCTATACCCCTGCTCCCAAGGAAGGGCATTCTTAAGAAGTCTCGGCAGCGTGAATCTGGTTACTA
  CTCCTCTCCAGAGCCCAGTGAGTCTGGGGAACTCTTAGACGCAGGTGATGTGTTTGTGAGTGGGGACCCC
  ATGGAGCAGAAGTCTCCACAAGCTTCAGGGCGCCTCCATCGCAAGGGCATCCTCAAACTCAATGGCAAGT
  TTTCCCGCACAGCCTTAGAAGGCACTGCCCCTAGCACCTTTGGCTCCCTAGACCAACTGGCCTCCCCTCA
  TCCTACAGCCCGGGCCAGCCGTCCCTCGGGAGCTGTGAGTGAGGACAGCATCCTGTCCTCCGAGTCCTTT
  GACCAATTGGACTTGCCCGAGCGGCTTCCCGAAACCCCACTGAGGGGCTGTGTGTCTGTGGACAACCTGA
  GGAGGCTTGAGCAGCCTCCCTCAGAAGGCCTAAAACGATGGTGGCAGGAATCCTTGGGGGATAGCTGCTT
  TTCTCTGACAGACTGCCAAGAGGTGACAGCAGCCTACAGACAAGCCCTAGGAATCTGCTCGAAGCTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212466 representing NM_001007617
  Red=Cloning site Green=Tags(s)
  
  MESVALHRRGNLAPSASALATESARPLADRLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGK
  VKKARESSGRLVAIKSIRKDKIKDEQDLLHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGD
  LYDYISERPRLNERDARHFFRQIVSALHYCHQNGIVHRDLKLENILLDASGNIKIADFGLSNLYHKGKFL
  QTFCGSPLYASPEIVNGKPYVGPEVDSWSLGVLLYILVHGTMPFDGQDHKTLVKQISSGAYREPCKPSDA
  CGLIRWLLMVNPIRRATLEDVASHWWVNWGYSTRIGEQEALREGGHPSGDSGRASMADWLRRSSRPLLEN
  GAKVCSFFKQHVPGGGSTGPGLERQHSLKKSRKENDMAQTLQNDPAEDTSSRPGKNSLKLPKGILKKKAS
  PSSGEVQEGPQELRPVSNTPGQPVPAIPLLPRKGILKKSRQRESGYYSSPEPSESGELLDAGDVFVSGDP
  MEQKSPQASGRLHRKGILKLNGKFSRTALEGTAPSTFGSLDQLASPHPTARASRPSGAVSEDSILSSESF
  DQLDLPERLPETPLRGCVSVDNLRRLEQPPSEGLKRWWQESLGDSCFSLTDCQEVTAAYRQALGICSKLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007617
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007617.1, NP_001007618.1
• RefSeq Size: 2900 bp
• RefSeq ORF: 1893 bp
• Locus ID: 289419
• UniProt ID: Q66HE5
• Cytogenetics: 13q13
• MW: 70 kDa
• Gene Summary: Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).[UniProtKB/Swiss-Prot Function]