Mgea5 (NM_131904) Rat Tagged ORF Clone

CAT#: RR211943

Mgea5 (Myc-DDK-tagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), (10 ug)

Product Images

Circular map for RR211943

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mgea5
• Synonyms: Ncoat; O-GLCNACASE; OGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211943 representing NM_131904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGGAGAGTCAAGCGGCGCTGGAGGAGCGGGAGAGTGAGCGCAACGCCAATCCTGCCTCCG
  TCTCGGGGGCTTCGTTGGAGCCGTCTGCAGCTCCGGCCCCCGGAGAAGACAACCCCTCGGGAGCTGGGGC
  GGCGGCCGGGACCGGGGCGGCCGGCGGGGCCCGCAGGTTCCTGTGCGGTGTAGTGGAAGGGTTTTATGGA
  AGACCTTGGGTTATGGAGCAGAGAAAAGAGCTCTTTAGAAGGCTTCAGAAATGGGAATTAAATACATACT
  TGTATGCTCCGAAGGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAAGAAGCTGA
  GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAATTCATCTATGCTATTTCTCCAGGA
  TTAGATATCACTTTTTCTAACCCAAAGGAAGTATCAACATTGAAACGCAAATTGGACCAGGTCTCCCAGT
  TTGGGTGCAGGTCATTTGCTTTGCTTTTTGACGATATTGATCATAACATGTGTGCAGCAGACAAAGAAGT
  GTTCAGTTCTTTTGCACATGCACAAGTTTCCATCACAAATGAAATCTATCAGTACCTGGGAGAGCCGGAA
  ACCTTCCTCTTCTGTCCCACAGAATACTGCGGCACTTTCTGTTATCCAAGTGTATCTCAGTCTCCTTACT
  TGAGGACTGTGGGTGAAAAGCTGCTACCTGGAATTGAGGTGCTTTGGACAGGTCCAAAAGTTGTTTCAAA
  AGAAATTCCAGTAGAATCTATTGAAGAAGTTTCTAAGATCATTAAGAGAGCCCCAGTGATCTGGGATAAC
  ATTCATGCTAATGACTATGATCAGAAGAGGCTGTTTCTGGGTCCCTACAAAGGAAGATCCACAGAGCTCA
  TCCCACGGTTGAAGGGAGTGCTGACTAATCCAAATTGTGAATTTGAAGCCAACTATGTTGCCATCCACAC
  CCTTGCCACTTGGTACAAGTCAAACATGAACGGAGTGAGGAAGGACGTGGTGATGACTGACAGTGAAGAC
  AGTACTGTCTCAATACAGATAAAGTTAGAAAATGAAGGCAGTGATGAAGACATTGAGACTGATGTGCTCT
  ACAGTCCACAGATGGCTTTAAAGCTGGCCTTAACTGAGTGGCTGCAGGAGTTCGGTGTACCGCATCAGTA
  CAGCAGTAGACAGGTTGCACACAGTGGAGCTAAGACAAGCGTAGTGGACGGGACTCCCTTGGTTGCAGCA
  CCTTCTTTAAATGCCACAACTGTAGTTACAACAGTTTACCAGGAGCCCATTATGAGCCAGGGAGCAGCTT
  TGAGTGGTGAGCCCTCAGCTCTGACCAAGGAAGAAGAAAAGAAACAGCCAGATGAGGAACCCATGGATAT
  GGTGGTAGAGAAGCAAGAAGAGTCAGAGCACAAGAGTGACAACCAGATACTAACTGAGATCGTCGAAGCT
  AAAATGGCAGAGGAGTTGAAACCAATGGACACTGATAAGGAAAGCATAGCCGAGTCAAAATCCCCAGAGA
  TGTCTATGCAGGAAGACTGCATTAACGACATCGCTCCTATGCAGACAGACGAGCAAGCAAACAAAGAGCA
  GTTTGTGCCAGGTCCAAATGAAAAACCTTTGTACGCTGCAGAACCAGTGACTCTGGAGGATTTACAGTTA
  CTTGCTGATCTCTTCTACCTTCCTTATGAGCATGGACCCAAAGGAGCACAGATGCTACGGGAGTTCCAGT
  GGCTTCGAGCAAACAGTAGTGTTGTCAGTGTCAATTGCAAAGGGAAAGACTCTGAAAAAATTGAAGAATG
  GCGGTCACGAGCAGCCAAGTTTGAAGAAATGTGCGCACTAGTGATGGGGATGTTCACCCGGCTCTCCAAC
  TGTGCCAACAGGACAATCCTTTATGACATGTACTCCTATGTGTGGGATATCAAGAGTATAATGTCTATGG
  TGAAGTCTTTTGTGCAGTGGTTAGGGTGTCGTAGTCATTCTTCAGCACAGTTCTTAATTGGAGACCAAGA
  ACCCTGGGCCTTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGAGCAAATGAT
  CTCTTTTTCCAACCACCCCCACTGACTCCTACCTCCAAGGTTTATACTATCAGACCTTATTTCCCTAAAG
  ATGAGGCTTCCGTGTACAAGATTTGCAGAGAAATGTATGATGATGGAGTGGGTTTACCTTTTCAAAGTCA
  GCCTGACCTTATTGGAGACAAGTTAGTAGGAGGACTGCTTTCCCTCAGCCTGGATTACTGTTTTGTCCTC
  GAAGATGAAGATGGTATTTGTGGTTATGCCTTAGGCACTGTAGATGTGACCCCCTTCATTAAAAAGTGCA
  AAATTTCCTGGATTCCCTTCATGCAGGAGAAGTATACTAAGCCAAATGGAGACAAAGAGCTCTCAGAAGC
  TGAGAAAATAATGTTGAGTTTCCATGAAGAGCAGGAGGTATTACCAGAAACTTTCCTTGCCAACTTCCCT
  TCTCTGATAAAAATGGATATTCATAAAAAGGTCACTGACCCAAGTGTTGCCAAAAGCATGATGGCTTGCC
  TCCTGTCTTCACTAAAGGCCAATGGCTCCCGGGGGGCTTTCTGTGAAGTAAGACCAGATGATAAAAGGAT
  TCTGGAATTTTACAGCAAGTTAGGCTGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTT
  ATACTTGGTCGAAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211943 representing NM_131904
  Red=Cloning site Green=Tags(s)
  
  MVQKESQAALEERESERNANPASVSGASLEPSAAPAPGEDNPSGAGAAAGTGAAGGARRFLCGVVEGFYG
  RPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPG
  LDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPE
  TFLFCPTEYCGTFCYPSVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDN
  IHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSED
  STVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAA
  PSLNATTVVTTVYQEPIMSQGAALSGEPSALTKEEEKKQPDEEPMDMVVEKQEESEHKSDNQILTEIVEA
  KMAEELKPMDTDKESIAESKSPEMSMQEDCINDIAPMQTDEQANKEQFVPGPNEKPLYAAEPVTLEDLQL
  LADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSN
  CANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGAND
  LFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVL
  EDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFP
  SLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVV
  ILGRSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_131904
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_131904.1, NP_571979.1
• RefSeq Size: 2751 bp
• RefSeq ORF: 2751 bp
• Locus ID: 154968
• UniProt ID: Q8VIJ5
• Cytogenetics: 1q54
• MW: 102.9 kDa
• Gene Summary: plays a role in insulin secretion; may be involved in secretion of other glucose regulated hormones [RGD, Feb 2006]