Mgea5 (NM_131904) Rat Untagged Clone

CAT#: RN211943

Mgea5 (untagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mgea5
• Synonyms: Ncoat; O-GLCNACASE; OGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211943 representing NM_131904
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGGAGAGTCAAGCGGCGCTGGAGGAGCGGGAGAGTGAGCGCAACGCCAATCCTGCCTCCG
  TCTCGGGGGCTTCGTTGGAGCCGTCTGCAGCTCCGGCCCCCGGAGAAGACAACCCCTCGGGAGCTGGGGC
  GGCGGCCGGGACCGGGGCGGCCGGCGGGGCCCGCAGGTTCCTGTGCGGTGTAGTGGAAGGGTTTTATGGA
  AGACCTTGGGTTATGGAGCAGAGAAAAGAGCTCTTTAGAAGGCTTCAGAAATGGGAATTAAATACATACT
  TGTATGCTCCGAAGGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAAGAAGCTGA
  GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAATTCATCTATGCTATTTCTCCAGGA
  TTAGATATCACTTTTTCTAACCCAAAGGAAGTATCAACATTGAAACGCAAATTGGACCAGGTCTCCCAGT
  TTGGGTGCAGGTCATTTGCTTTGCTTTTTGACGATATTGATCATAACATGTGTGCAGCAGACAAAGAAGT
  GTTCAGTTCTTTTGCACATGCACAAGTTTCCATCACAAATGAAATCTATCAGTACCTGGGAGAGCCGGAA
  ACCTTCCTCTTCTGTCCCACAGAATACTGCGGCACTTTCTGTTATCCAAGTGTATCTCAGTCTCCTTACT
  TGAGGACTGTGGGTGAAAAGCTGCTACCTGGAATTGAGGTGCTTTGGACAGGTCCAAAAGTTGTTTCAAA
  AGAAATTCCAGTAGAATCTATTGAAGAAGTTTCTAAGATCATTAAGAGAGCCCCAGTGATCTGGGATAAC
  ATTCATGCTAATGACTATGATCAGAAGAGGCTGTTTCTGGGTCCCTACAAAGGAAGATCCACAGAGCTCA
  TCCCACGGTTGAAGGGAGTGCTGACTAATCCAAATTGTGAATTTGAAGCCAACTATGTTGCCATCCACAC
  CCTTGCCACTTGGTACAAGTCAAACATGAACGGAGTGAGGAAGGACGTGGTGATGACTGACAGTGAAGAC
  AGTACTGTCTCAATACAGATAAAGTTAGAAAATGAAGGCAGTGATGAAGACATTGAGACTGATGTGCTCT
  ACAGTCCACAGATGGCTTTAAAGCTGGCCTTAACTGAGTGGCTGCAGGAGTTCGGTGTACCGCATCAGTA
  CAGCAGTAGACAGGTTGCACACAGTGGAGCTAAGACAAGCGTAGTGGACGGGACTCCCTTGGTTGCAGCA
  CCTTCTTTAAATGCCACAACTGTAGTTACAACAGTTTACCAGGAGCCCATTATGAGCCAGGGAGCAGCTT
  TGAGTGGTGAGCCCTCAGCTCTGACCAAGGAAGAAGAAAAGAAACAGCCAGATGAGGAACCCATGGATAT
  GGTGGTAGAGAAGCAAGAAGAGTCAGAGCACAAGAGTGACAACCAGATACTAACTGAGATCGTCGAAGCT
  AAAATGGCAGAGGAGTTGAAACCAATGGACACTGATAAGGAAAGCATAGCCGAGTCAAAATCCCCAGAGA
  TGTCTATGCAGGAAGACTGCATTAACGACATCGCTCCTATGCAGACAGACGAGCAAGCAAACAAAGAGCA
  GTTTGTGCCAGGTCCAAATGAAAAACCTTTGTACGCTGCAGAACCAGTGACTCTGGAGGATTTACAGTTA
  CTTGCTGATCTCTTCTACCTTCCTTATGAGCATGGACCCAAAGGAGCACAGATGCTACGGGAGTTCCAGT
  GGCTTCGAGCAAACAGTAGTGTTGTCAGTGTCAATTGCAAAGGGAAAGACTCTGAAAAAATTGAAGAATG
  GCGGTCACGAGCAGCCAAGTTTGAAGAAATGTGCGCACTAGTGATGGGGATGTTCACCCGGCTCTCCAAC
  TGTGCCAACAGGACAATCCTTTATGACATGTACTCCTATGTGTGGGATATCAAGAGTATAATGTCTATGG
  TGAAGTCTTTTGTGCAGTGGTTAGGGTGTCGTAGTCATTCTTCAGCACAGTTCTTAATTGGAGACCAAGA
  ACCCTGGGCCTTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGAGCAAATGAT
  CTCTTTTTCCAACCACCCCCACTGACTCCTACCTCCAAGGTTTATACTATCAGACCTTATTTCCCTAAAG
  ATGAGGCTTCCGTGTACAAGATTTGCAGAGAAATGTATGATGATGGAGTGGGTTTACCTTTTCAAAGTCA
  GCCTGACCTTATTGGAGACAAGTTAGTAGGAGGACTGCTTTCCCTCAGCCTGGATTACTGTTTTGTCCTC
  GAAGATGAAGATGGTATTTGTGGTTATGCCTTAGGCACTGTAGATGTGACCCCCTTCATTAAAAAGTGCA
  AAATTTCCTGGATTCCCTTCATGCAGGAGAAGTATACTAAGCCAAATGGAGACAAAGAGCTCTCAGAAGC
  TGAGAAAATAATGTTGAGTTTCCATGAAGAGCAGGAGGTATTACCAGAAACTTTCCTTGCCAACTTCCCT
  TCTCTGATAAAAATGGATATTCATAAAAAGGTCACTGACCCAAGTGTTGCCAAAAGCATGATGGCTTGCC
  TCCTGTCTTCACTAAAGGCCAATGGCTCCCGGGGGGCTTTCTGTGAAGTAAGACCAGATGATAAAAGGAT
  TCTGGAATTTTACAGCAAGTTAGGCTGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTT
  ATACTTGGTCGAAGCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_131904
• Insert Size: 2751 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_131904.1, NP_571979.1
• RefSeq Size: 2751 bp
• RefSeq ORF: 2751 bp
• Locus ID: 154968
• UniProt ID: Q8VIJ5
• Cytogenetics: 1q54
• Gene Summary: plays a role in insulin secretion; may be involved in secretion of other glucose regulated hormones [RGD, Feb 2006]