Papd7 (NM_001107333) Rat Tagged ORF Clone

CAT#: RR211221

Papd7 (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed) sigma (Pols), (10 ug)

Product Images

Circular map for RR211221

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Papd7
• Synonyms: Pols
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211221 representing NM_001107333
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCTTGTCCTGAAGAAGCAGCCATGAGAAGGGAGGTGGTGAAGCGCATCGAAACTGTGGTGAAAG
  ACCTCTGGCCCTCGGCTGACGTGCAGATATTTGGCAGCTTTAGCACAGGCCTCTATCTTCCAACAAGTGA
  CATAGACCTAGTGGTCTTTGGAAAGTGGGAGCGACCTCCATTACAGCTGCTGGAACAAGCCCTGCGGAAG
  CACAACGTGGCTGAACCATGCTCCATCAAAGTCCTTGACAAAGCTACAGTGCCCATTATAAAGCTCACAG
  ATCAGGAGACTGAAGTTAAAGTCGACATCAGCTTTAACATGGAGACTGGTGTACGGGCGGCAGAGTTCAT
  CAAGAATTACATGAAGAAGTACTCGCTACTGCCATACTTGATTTTAGTGTTGAAACAGTTCCTCCTGCAG
  AGGGACCTGAATGAAGTCTTCACAGGCGGGATCAGCTCCTACAGCCTCATCTTAATGGCCATCAGCTTTC
  TGCAGTTACATCCAAGAATTGATGCCCGGAGAGCTGATGAAAACCTGGGAATGCTTCTTGTAGAATTTTT
  TGAACTTTATGGAAGGAATTTTAATTACTTGAAAACTGGTATTAGAATAAAAGAAGGAGGTGCCTATATC
  GCCAAGGAAGAGATTATGAAAGCCATGACCAGTGGGTACCGACCGTCGATGCTGTGCATTGAGGACCCCC
  TACTGCCTGGAAACGATGTTGGGCGGAGTTCCTATGGGGCCATGCAGGTGAAGCAGGTGTTTGACTATGC
  GTACATTGTGCTCAGCCATGCCGTGTCACCGCTCGCCAGGTCTTACCCCAACAGGGATTCTGAAAGTACT
  TTAGGAAGAATAATCAAAGTCACCCAGGAAGTGATCGACTACCGGAGGTGGATCAAAGAAAAATGGGGTA
  GCAGAATCGTCCCGTCTCCAGACCTAGACAACAGGATTAAGATAAAGGAGAGAGTCACCACGTGCAATGG
  GGAGCAGATGCAGAGCCGGGAGCCCAGCTCCCCTTACAGCCAGCGCCTGACTCTGTCCCTGTCCAGCCCC
  CAGCTGCTGTCTTCAGGCTCCTCTGCTTCTTCTGTGTCCTCGCTTTCTGGAAGTGACATTGATTCAGACA
  CACCACCCTGCACCACGCCCAGTGTTTATCAGTTCAGCCTGCAAGCACCCACCACCCTGATGGCCGGCCT
  GCCCACAGCCTTGCCAGTGCCCAGCAGCAAGCCCCAGCCAGCTGCTTCCAGAACGTTGATCATGACAACC
  AACAATCAGACCAGAGTAACTATCCCTCCACCGACCCTCGGAGTTGCCCCTGTACCTTGCAGACAGGCTG
  GTGTCGACGGAACCACATCTTTGAAGGCCGTTCACAACGTAACTTCCCCAGCCATTCCCTCGGCATCCCC
  CAACCCACTGTCTAGTCCTCATCTCTATCATAAGCACAATGGCATGAAACTGTCCATGAAAGGTTCCCAC
  AACCACACTCAGGGTGGTGGCTACAGCTCTGTGGGCAGTGGAGCTGTGAGGCCTCCTGTTGGTAACCGAG
  GACACCATCAGTACAACCGTACCGGCTGGAGGAGGAAAAAGCATGCACACACAAGGGACAGCCTGCCTGT
  GAGTCTCAGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211221 representing NM_001107333
  Red=Cloning site Green=Tags(s)
  
  MSPCPEEAAMRREVVKRIETVVKDLWPSADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRK
  HNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETGVRAAEFIKNYMKKYSLLPYLILVLKQFLLQ
  RDLNEVFTGGISSYSLILMAISFLQLHPRIDARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYI
  AKEEIMKAMTSGYRPSMLCIEDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDSEST
  LGRIIKVTQEVIDYRRWIKEKWGSRIVPSPDLDNRIKIKERVTTCNGEQMQSREPSSPYSQRLTLSLSSP
  QLLSSGSSASSVSSLSGSDIDSDTPPCTTPSVYQFSLQAPTTLMAGLPTALPVPSSKPQPAASRTLIMTT
  NNQTRVTIPPPTLGVAPVPCRQAGVDGTTSLKAVHNVTSPAIPSASPNPLSSPHLYHKHNGMKLSMKGSH
  NHTQGGGYSSVGSGAVRPPVGNRGHHQYNRTGWRRKKHAHTRDSLPVSLSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107333
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107333.1, NP_001100803.1
• RefSeq Size: 3819 bp
• RefSeq ORF: 1626 bp
• Locus ID: 306672
• Cytogenetics: 1p11
• MW: 59.8 kDa