Cdk17 (NM_001108082) Rat Tagged ORF Clone

CAT#: RR211057

Cdk17 (Myc-DDK-tagged ORF) - Rat PCTAIRE protein kinase 2 (Pctk2), (10 ug)

Product Images

Circular map for RR211057

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdk17
• Synonyms: Pctaire2; Pctk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211057 representing NM_001108082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAGTTTAAGAGGAGACTGTCCCTCACGCTCAGGGGAAGCCAGACTATTGATGAGTCCCTGTCGG
  AGCTGGCTGAACAGATGACCATAGAGGAGAGCAGTAGCAAAGACAACGAGCCCATTGTGAAGAATGGCAG
  GCCGCCAACGTCTCACAGCATGCATTCCTTCCTCCATCAGTACACAGGGTCCTTCAAGAAGCCCCCACTG
  CGAAGACCCCACAGCGTTATTGGAGGAAGCCTTGGTTCCTTTATGGCAATGCCCAGAAATGGAAGCAGAT
  TAGATATTGTTCATGAAAATCTGAAAATGGGATCAGATGGTGAGAGTGACCAAGCTTCTGGGACATCATC
  TGATGAAGTCCAGTCCCCTACAGGTGTTTGCCTCAGAAATCGTATACATAGACGGATCTCGATGGAGGAC
  TTGAATAAGCGGCTGTCGCTGCCTGCAGACATCAGGATACCCGATGGCTACCTGGAGAAGCTGCAGATCA
  GCAGCCCGCCCTTCGACCAGCCCATGAGCCGCAGGTCCCGCAGAGCCTCCTTATCAGAAATTGGGTTTGG
  GAAAATGGAAACCTACATCAAATTGGAAAAGCTTGGAGAGGGCACATATGCAACGGTATATAAAGGGAGA
  AGTAAGTTGACAGAGAACTTGGTGGCATTAAAAGAGATCCGCTTGGAACACGAAGAAGGTGCACCCTGTA
  CAGCTATAAGAGAAGTTTCACTGTTAAAGGACTTAAAACATGCAAATATAGTAACGTTGCATGACATCGT
  TCACACGGACAAGTCTTTGACTCTGGTCTTTGAGTACCTGGACAAAGACCTGAAGCAGTACATGGACGAC
  TGTGGGAGCATCATGAGCATGCACAACGTCAAGCTGTTCCTGTACCAGATTCTCCGTGGTTTGGCATATT
  GCCATAGAAGAAAAGTGTTGCACCGGGACCTGAAACCACAGAACCTCCTCATTAATGAAAGAGGAGAACT
  AAAGCTGGCTGACTTTGGACTAGCCAGAGCCAAGTCAGTCCCCACCAAGACCTACTCAAATGAAGTTGTC
  ACGCTGTGGTATCGGCCCCCTGACGTGCTTCTTGGTTCCTCGGAGTACTCAACTCAGATCGACATGTGGG
  GAGTTGGCTGCATTTTCTTTGAAATGGCTTCTGGAAGACCACTGTTTCCAGGGTCAACTGTGGAAGATGA
  ACTGCACTTAATATTTCGACTACTAGGAACTCCATCTCAGGAAACTTGGCCAGGTGTTTCTTCAAATGAT
  GAATTCAAGAACTACAACTTCCCAAAATATAAGCCACAACCTCTAATTAATCATGCACCCAGGTTAGATT
  CTGAAGGAATTGAGTTGATAACAAAGTTTCTTCAGTATGAATCTAAGAAAAGGGCTCCAGCAGAAGAGGC
  CATGAAACATGTGTACTTCCGAAGTCTAGGGCCGAGAATACATGCTTTACCAGAAAGCGTATCAATATTC
  AGTTTGAAAGAGATTCAGTTGCAAAAGGACCCGGGTTTTCGAAATTCTTCCTATCCAGAGACAGGACATG
  GGAAGAACAGAAGACAGAGCATGCTCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211057 representing NM_001108082
  Red=Cloning site Green=Tags(s)
  
  MKKFKRRLSLTLRGSQTIDESLSELAEQMTIEESSSKDNEPIVKNGRPPTSHSMHSFLHQYTGSFKKPPL
  RRPHSVIGGSLGSFMAMPRNGSRLDIVHENLKMGSDGESDQASGTSSDEVQSPTGVCLRNRIHRRISMED
  LNKRLSLPADIRIPDGYLEKLQISSPPFDQPMSRRSRRASLSEIGFGKMETYIKLEKLGEGTYATVYKGR
  SKLTENLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIVHTDKSLTLVFEYLDKDLKQYMDD
  CGSIMSMHNVKLFLYQILRGLAYCHRRKVLHRDLKPQNLLINERGELKLADFGLARAKSVPTKTYSNEVV
  TLWYRPPDVLLGSSEYSTQIDMWGVGCIFFEMASGRPLFPGSTVEDELHLIFRLLGTPSQETWPGVSSND
  EFKNYNFPKYKPQPLINHAPRLDSEGIELITKFLQYESKKRAPAEEAMKHVYFRSLGPRIHALPESVSIF
  SLKEIQLQKDPGFRNSSYPETGHGKNRRQSMLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108082
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108082.2, NP_001101552.2
• RefSeq Size: 3670 bp
• RefSeq ORF: 1572 bp
• Locus ID: 314743
• Cytogenetics: 7q13
• MW: 59.5 kDa