Brd8 (NM_001008509) Rat Tagged ORF Clone

CAT#: RR210707

Brd8 (Myc-DDK-tagged ORF) - Rat bromodomain containing 8 (Brd8), (10 ug)

Product Images

Circular map for RR210707

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brd8
• Synonyms: p120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210707 representing NM_001008509
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTGCAGCGTGTCAATCTCTGCTCTGTGAGTGGCACTCCTGAGGTATGGCCACTATGGGGTCTTT
  CAACAGTAATATCTTTCCGTGTGGTTCCTGGATCAAACTCAGGTTGTAGGGCTTTTAGAACAAGGGTATC
  AGTTAGCAGAGCAATCAAGCCCTTTGCTGAACCTGGCCGGCCTCCAGACTGGTTCTCTCAAAAACATTGT
  GCTTCTCAGTATTCTGAGCTCTTAGAGACTACTGAGACTCCAAAACGAAAACGGGGTGAAAAGGGAGAAG
  TGGTAGAAACTGTTGAAGATGTTATTGTTCGCAAACTGACTGCTGAGAGAGTTGAGGAACTGAAGAAAGT
  CATAAAGGAGACACAGGAGAGATACAGGCGTCTGAAAAGAGATGCAGAACTAATTCAAGCCGGGCACATG
  GACAGCAGACTGGATGAGCTCTGCAGTGACATTGCAATGAAAAAGAAATTGGAGGAGGAAGAGGCTGAAG
  TAAAAAGGAAGGCCACAGATGCTGCATACCAGGCTCGGCAAGCAGTGAAAACACCTCCTCGAAGGTTGCC
  AACTGTGATGGTCCGCTCCCCTATAGACTCCGCCTCCCCAGGAGGTGATTATCCACTTGGAGACTTGACT
  CCAACCACTATGGAAGAGGCTACCTCTGGAGTAACCCCTGGGACTTTGCCGAGTACCCCAGTCACCTCGT
  TTCCTGGGATTCCTGACACCCTTCCTCCAGGCTCTGCACCCTTAGAAGCCCCCATGACCCCAGTAACAGA
  TGATTCACCCCAGAAAAAGATGCTTGGACAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTC
  TTGAAGAAGGGCAGCCTCCTGCCTACTAGCCCCAGACTGGTGAATGAGAGTGAAATGCCCGTGCCCTCTG
  GCCATCTGAGCAGCACAGGGGTTCTCCTGGAGGTAGGCGGGGTTCTTCCCATGATCCATGGTGGGGACAT
  ACAGCCAGCAACCAGTGCAGTGGCAGCCTCCCCCGCTGCCTCAGGTGCTCCCACTCTCTCCCGGCTTTTA
  GAAGCTGGTCCCACACAGTTCTCCACTCCTCTTCCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTA
  AGCTTGTGCCACCCCCTGTAGAGTCTGTGTCCCAGGCTACCATTGTCATGATGCCTGCGCTGCCAGCACC
  GTCCTCTGCTCCGGCTGTCTCCACTCCTGAAAGTGTAGCTCCAGTGAGCCAGCCTGAGCCCTGTGTTCCC
  CTGGAAGCCGTGGGGGATCCACAGACTGTGACTGTTTCCATGGATAGCAGTGAAATCTCCATGATCATTA
  ATTCCATCAAAGAAGAGTGTTTCCGCTCAGGAGTAGCAGAGGCTCCTGGTGGATCAAAGGCTCCAAGCAT
  AGATGGGAAGGAAGATTTAGATCTGGCTGAAAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGTTG
  GACTTTGAAACAGTTGGAGACATCATTGCCATCATTGAGGACAAGGTGGATGACCACCCTGAAGTGCTGG
  ATGTGGCAGCTGTAGAAGCAGCATTGTCGTTCTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCC
  TTGGGAGCACCCTATCCAGCAGGAGCGGGACAAGCCAGTGCCTCTTCCAGCACCAGAGATGACAGTCAAA
  CAAGAGAGGCTAGACTTCGAGGAGTCAGAAACCAAAGGGATCCATGAACTGGTGGACATCAGGGATTCAG
  GTGTTGAGATAAAGGTGGAACCTACAGAGCCAGAGCCAGGCATGTCCGGGGCTGAGATCGTAGCTGGAGT
  TGGTCCAGTCCCAAGTTTGGAGCCACCAGAACTCAGGAGTCAAGACTTAGATGAGGAACCTAGAAGTTCT
  GCAGCTGGAGAGATGGGTGAGGCAGACGTTTCCAGTGGGAAAGGCGAGGAAAGGCCACTTCCGGCTGTGA
  AGACAGAGGCGTCCCCTGAAAGCATGTTGTCTCCATCACATGGCTCAAATCCTATTGAAGATCCTTTAGA
  GGCAGAGACTCAACACAAGTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGC
  CAGATAAAGGACGCTCCAGGTGAGGAGGAGGAGGAGGATGGAGTCAGTGAAGCAGCTAGCCTGGAGGAGC
  CTAAGGAGGAGGATCAAGGCGAAGGCTATTTGTCTGAGATGGACAACGAGCCCCCTGTGAGCGAAAGCGA
  TGATGGCTTTAGTATACATAATGCCACACTGCAGTCACACACTCTGGGCGACTCCATCCCGAGCAGCCCC
  GCCTCCTCGCAGTTTTCCGTGTGCAGTGAAGATCAAGAAGCAATTCAGGCTCAGAAAATATGGAAGAAAG
  CCATCATGCTTGTATGGAGAGCTGCAGCCAACCATCGGTATGCCAATGTGTTCCTGCAGCCTGTCACAGA
  CGACATAGCTCCTGGCTACCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATAAAGAAAAACATT
  GAAAACGGACTGATCCGCAGCACAGCCGAGTTTCAGCGTGATATCATGCTGATGTTCCAGAACGCTGTTA
  TGTACAATAGCTCAGACCATGATGTCTACCACATGGCAGTGGAGATGCAGAGAGACGTCTTGGAACAGAT
  CCAGCAATTCTTGGCCACACAGTTGATTATGCAAACATCTGAGTCTGGAATCAGTGCTAAAAGTCTCCGG
  GGGAGAGACTCTACCCGAAAACAAGATGCTTCAGAGAAGGACAGTGTCCCCATGGGCTCTCCTGCCTTCC
  TTCTCTCTCTGTTTGATGGGGGAACCAGGGGCCGCCGCTGTGCCATCGAAGCGGATATGAAGATGAAGAA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210707 representing NM_001008509
  Red=Cloning site Green=Tags(s)
  
  MLLQRVNLCSVSGTPEVWPLWGLSTVISFRVVPGSNSGCRAFRTRVSVSRAIKPFAEPGRPPDWFSQKHC
  ASQYSELLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHM
  DSRLDELCSDIAMKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLT
  PTTMEEATSGVTPGTLPSTPVTSFPGIPDTLPPGSAPLEAPMTPVTDDSPQKKMLGQKATPPPSPLLSEL
  LKKGSLLPTSPRLVNESEMPVPSGHLSSTGVLLEVGGVLPMIHGGDIQPATSAVAASPAASGAPTLSRLL
  EAGPTQFSTPLPSFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTPESVAPVSQPEPCVP
  LEAVGDPQTVTVSMDSSEISMIINSIKEECFRSGVAEAPGGSKAPSIDGKEDLDLAEKMDIAVSYTGEEL
  DFETVGDIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVK
  QERLDFEESETKGIHELVDIRDSGVEIKVEPTEPEPGMSGAEIVAGVGPVPSLEPPELRSQDLDEEPRSS
  AAGEMGEADVSSGKGEERPLPAVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGS
  QIKDAPGEEEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLGDSIPSSP
  ASSQFSVCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNI
  ENGLIRSTAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLR
  GRDSTRKQDASEKDSVPMGSPAFLLSLFDGGTRGRRCAIEADMKMKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008509
• ORF Size: 2871 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008509.1, NP_001008509.1
• RefSeq Size: 3318 bp
• RefSeq ORF: 2874 bp
• Locus ID: 291691
• Cytogenetics: 18p12
• MW: 103.3 kDa