Kcns2 (NM_023966) Rat Tagged ORF Clone

CAT#: RR210633

Kcns2 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2 (Kcns2), (10 ug)

Product Images

Circular map for RR210633

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcns2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210633 representing NM_023966
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCGCCAGAGCCTGTGGGACCTGTCTGAGACCGACGTCGAGGATGGAGAGATCCGAATCAATGTAG
  GCGGCTTCAAGAGACGGCTGCGTTCCCACACGCTGCTGCGCTTCCCTGAGACACGCCTGGGCCGTCTGCT
  CCTCTGCCACTCGAGAGAGGCCATTCTGGAACTCTGCGATGACTACGACGATGTCCAGCGTGAGTTCTAC
  TTCGACCGTAACCCTGAGCTCTTCCCCTACGTGTTGCATTTCTACCACACCGGCAAGCTCCATGTCATGG
  CCGAGCTGTGCGTCTTCTCCTTCAGCCAGGAGATCGAGTACTGGGGTATCAACGAGTTCTTTATTGACTC
  TTGCTGCAGCTACAGCTATCACGGCCGCAAAGTGGAACCTGAGCAGGAGAAGTGGGACGAGCAGAGTGAC
  CAGGAAAGCACTACTTCCTCCTTCGACGAGATCTTAGCCTTCTATAATGATGCTTCCAAGTTCGATGGGC
  AACCCCTGGGCAACTTCCGCAGGCAGCTGTGGCTGGCGTTGGATAACCCGGGCTACTCAGTCCTGAGCAG
  GGTCTTCAGCGTCCTTTCCATCTTGGTGGTGTTGGGCTCCATCATCACCATGTGCCTCAACAGCCTGCCA
  GACTTCCAGATCCCCGATAGCCAGGGTAACCCTGGCGAAGACCCCAGGTTCGAAATCGTGGAGCACTTTG
  GCATTGCCTGGTTCACGTTTGAGTTGGTGGCCAGGTTCGCTGTGGCCCCTGACTTTCTCAAGTTCTTCAA
  GAATGCCCTAAACCTTATTGACCTCATGTCCATTGTCCCGTTTTACATAACTCTAGTGGTGAACCTGGTG
  GTGGAGAGTTCGCCTACCTTGGCTAACTTGGGCAGGGTGGCTCAAGTCCTGAGGCTGATGAGAATCTTCC
  GAATTCTCAAGCTGGCCAGACACTCCACTGGCCTCCGGTCCTTGGGGGCCACCCTGAAGTACAGCTACAA
  GGAAGTGGGGTTGCTCTTGCTCTACCTCTCGGTGGGGATTTCCATCTTCTCTGTGGTGGCCTACACCATT
  GAAAAGGAAGAGAACGAGGGCCTGGCCACCATTCCTGCCTGCTGGTGGTGGGCCACGGTCAGCATGACTA
  CCGTTGGGTATGGAGACGTGGTCCCAGGGACCACAGCTGGGAAGCTGACTGCCTCTGCCTGCATCTTGGC
  AGGTATCCTGGTGGTGGTCTTGCCCATCACTTTGATCTTCAATAAGTTCTCCCACTTTTATCGGCGCCAG
  AAGCAACTTGAGAGCGCCATGCGCAGCTGTGACTTTGGGGATGGAATGAAGGAGGTCCCCTCCGTCAATT
  TAAGGGACTACTATGCTCATAAAGTTAAGTCCCTCATGGCAAGCCTGACAAATATGAGTAGGAGTTCACC
  CAGTGAACTGAGTTTAGACGACTCTCTACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210633 representing NM_023966
  Red=Cloning site Green=Tags(s)
  
  MTRQSLWDLSETDVEDGEIRINVGGFKRRLRSHTLLRFPETRLGRLLLCHSREAILELCDDYDDVQREFY
  FDRNPELFPYVLHFYHTGKLHVMAELCVFSFSQEIEYWGINEFFIDSCCSYSYHGRKVEPEQEKWDEQSD
  QESTTSSFDEILAFYNDASKFDGQPLGNFRRQLWLALDNPGYSVLSRVFSVLSILVVLGSIITMCLNSLP
  DFQIPDSQGNPGEDPRFEIVEHFGIAWFTFELVARFAVAPDFLKFFKNALNLIDLMSIVPFYITLVVNLV
  VESSPTLANLGRVAQVLRLMRIFRILKLARHSTGLRSLGATLKYSYKEVGLLLLYLSVGISIFSVVAYTI
  EKEENEGLATIPACWWWATVSMTTVGYGDVVPGTTAGKLTASACILAGILVVVLPITLIFNKFSHFYRRQ
  KQLESAMRSCDFGDGMKEVPSVNLRDYYAHKVKSLMASLTNMSRSSPSELSLDDSLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023966
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023966.1, NP_076456.1
• RefSeq Size: 1660 bp
• RefSeq ORF: 1434 bp
• Locus ID: 66022
• UniProt ID: Q9ER26
• Cytogenetics: 7q22
• MW: 54.3 kDa
• Gene Summary: human homolog is a alpha subunit of a delayed-rectifier potassium channel [RGD, Feb 2006]