Cd4 (NM_012705) Rat Tagged ORF Clone

CAT#: RR210599

Cd4 (Myc-DDK-tagged ORF) - Rat Cd4 molecule (Cd4), (10 ug)

Product Images

Circular map for RR210599

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd4
• Synonyms: p55; W3/25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210599 representing NM_012705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCGAGGCTTCTCTTTCAGGCACTTGCTGCCGCTGCTGCTCCTGCAGCTGTCAAAACTCCTAGTTG
  TCACCCAAGGAAAGACCGTGGTGCTGGGGAAGGAAGGGGGTTCAGCAGAACTGCCCTGTGAAAGTACCTC
  GAGGAGGAGTGCATCCTTCGCGTGGAAGTCCTCTGACCAAAAGACAATTCTGGGATATAAGAACAAGTTA
  TTGATTAAAGGTTCACTTGAGCTGTATAGTCGTTTTGATTCCAGAAAAAATGCATGGGAGAGAGGATCAT
  TTCCCCTCATCATCAATAAACTTAGGATGGAGGACTCTCAGACTTATGTCTGCGAGCTGGAGAACAAGAA
  AGAGGAGGTGGAGTTGTGGGTCTTCAGAGTGACCTTCAATCCGGGTACCAGACTGTTGCAGGGGCAGAGC
  CTGACCCTGATCTTGGATAGCAACCCTAAGGTCTCTGACCCCCCGATAGAGTGCAAACACAAAAGCAGTA
  ACATTGTCAAGGACTCCAAAGCTTTCTCCACGCACAGCCTAAGGATTCAGGACAGTGGCATCTGGAACTG
  CACCGTGACCCTGAACCAGAAGAAGCACTCATTTGACATGAAACTCTCAGTGCTGGGCTTTGCGAGCACA
  TCCATCACGGCCTATAAGAGTGAGGGGGAGTCAGCGGAGTTCTCCTTCCCACTCAACCTTGGAGAGGAAA
  GCCTGCAGGGAGAGTTGAGATGGAAGGCAGAGAAGGCTCCTTCTTCCCAGTCCTGGATCACCTTCTCCCT
  AAAGAACCAAAAGGTGTCTGTGCAGAAGTCTACTAGCAACCCCAAGTTCCAGCTGTCCGAAACGCTCCCA
  CTCACCCTTCAGATACCCCAGGTCTCCCTTCAGTTTGCTGGTTCTGGCAACCTGACCCTGACTCTGGACA
  GAGGGATACTGTATCAGGAAGTGAACCTGGTGGTGATGAAAGTGACTCAGCCCGACAGCAACACTTTGAC
  CTGTGAGGTGATGGGACCCACCTCACCCAAGATGAGACTGATCTTGAAGCAGGAGAATCAGGAGGCCAGG
  GTCTCCAGGCAGGAGAAAGTGATTCAAGTGCAGGCCCCTGAAGCAGGGGTGTGGCAATGTCTACTGAGTG
  AAGGTGAAGAGGTCAAGATGGACTCCAAGATCCAGGTTTTATCCAAAGGGTTGAACCAGACAATGTTCCT
  GGCTGTCGTGCTGGGGAGCGCCTTCAGCTTTCTGGTTTTCACGGGGCTCTGCATCCTATTCTGTGTCAGG
  TGCCGGCACCAACAGCGCCAGGCAGCACGGATGTCTCAGATCAAGAGGCTTCTCAGTGAGAAGAAGACTT
  GCCAGTGCTCCCACCGGATGCAGAAAAGCCACAATCTCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210599 representing NM_012705
  Red=Cloning site Green=Tags(s)
  
  MCRGFSFRHLLPLLLLQLSKLLVVTQGKTVVLGKEGGSAELPCESTSRRSASFAWKSSDQKTILGYKNKL
  LIKGSLELYSRFDSRKNAWERGSFPLIINKLRMEDSQTYVCELENKKEEVELWVFRVTFNPGTRLLQGQS
  LTLILDSNPKVSDPPIECKHKSSNIVKDSKAFSTHSLRIQDSGIWNCTVTLNQKKHSFDMKLSVLGFAST
  SITAYKSEGESAEFSFPLNLGEESLQGELRWKAEKAPSSQSWITFSLKNQKVSVQKSTSNPKFQLSETLP
  LTLQIPQVSLQFAGSGNLTLTLDRGILYQEVNLVVMKVTQPDSNTLTCEVMGPTSPKMRLILKQENQEAR
  VSRQEKVIQVQAPEAGVWQCLLSEGEEVKMDSKIQVLSKGLNQTMFLAVVLGSAFSFLVFTGLCILFCVR
  CRHQQRQAARMSQIKRLLSEKKTCQCSHRMQKSHNLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012705
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012705.1, NP_036837.1
• RefSeq Size: 1749 bp
• RefSeq ORF: 1374 bp
• Locus ID: 24932
• UniProt ID: P05540
• Cytogenetics: 4q42
• MW: 51.4 kDa
• Gene Summary: MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]