Rnf168 (NM_001127597) Rat Tagged ORF Clone

CAT#: RR210589

Rnf168 (Myc-DDK-tagged ORF) - Rat ring finger protein 168 (Rnf168), (10 ug)

Product Images

Circular map for RR210589

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf168
• Synonyms: MGC188789
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210589 representing NM_001127597
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGGCTGCACCTAAAAACTCCATCCCTTCCTTAGCGGAATGTCAGTGCGGGATCTGCATGGAAA
  TCCTCGTAGAGCCTGTTACCCTACCTTGCAACCACACGCTCTGTAACCCATGCTTCCAATCGACTGTTGA
  AAAGGCCAATTTATGCTGTCCCTTCTGTCGCCGCCGGGTCTCTTCGTGGACTCGGTACCATACCCGAAGA
  AATTCTCTGGTCAATACAGACCTGTGGGAGATTATTCAAAAGCACTATGCAAAGGAATGCAAGCTTAGAA
  TCTCTGGACAAGAATCAAAGGAAATCGTTGATGAGTACCAGCCAGTTCGTCTACTCAGTAAACCTGGGGA
  GTTGAGGCGAGAATATGAAGAGGAGATAAGCAAGGTGGAGGCTGAGCGACAAGCCAGCAAGGAAGAGGAA
  AACAAAGCCAGTGAGGAGTACATACAGAGACTGCTGGCCGAGGAGGAGGAGGAGGAGAAAAGGCGGACGG
  AGAGAAGACGAAGTGAGATGGAGGAGCAGCTGAGGGGCGATGAGGAGCTGGCGAGGAGGCTGAGCACCAG
  CATCAACAGTAACTACGAGAGAAATATCTTGGCATCTCCTTTGAGTTCCAGAAAATCAGATCCCGTCACA
  AACAAGTCACAGAAGAAAAATACGAACAAACAAAAAAACTTTGGAGATATTCAAAGATACTTGTCACCTA
  AGTCAAAGCCTGGGACAGCATGGGCATGTAAAACTGAGCATGGAGAAGACATGTGCAAGTCTAAGGAAAC
  AGACAGTAGTGACACGAAGAGCCCTGTGTTGCAAGACACAGACGTTGAAGAAAGCATGCCAACACATTCT
  CCTCAGACCTGCCCAGAAACTCAAGGGCAAGGTCCGGAGCCTTTGACAGAGATGCCTGTGCCATGGCTAT
  GTGCAAGGAATGCTGAACAGTGCCTTGAGGGAAAAGCTGAAGCAGTGTCAACCAATCCTGATGATTCATG
  TATTGTAAATGATGGTGGACCTAGAGCCATAGTTTCTAACTCTAAGGAAGCTGCAGTTAAGCCTCCTACC
  AAGATAGAAAACGAAGAGTATTCTGTGTCAGGTGTGACCCAGTTAACTGGGGGCAACGGAGTTCCAACAG
  AGAGCAGAGTGTACGACTTACTAGTCGGAAAAGAGATTTCCGAAAGAGAAAACCAGGAATCTGTGTTTGA
  AGAAGTCATGGATCCATGCTTTTCTGCAAAAAGAAGGAAAATATTCATCACATCCTCCTTAGATCAAGAA
  GAAACAGAAGTGAATTTTACACAAAAACTGATAGATTTGGAACATATGCTTTTCGAGAGACATAAGCAAG
  AAGAACAGGACAGGTTGTTAGCATTACAACTTCAAAAAGAGGCAGATAAAGAAAAAATGGTGCCAAACCG
  GCAGAAAGGATCCCCAGATCAGTACCAGCTGCGCACATCTTCACCCCCAGACGGGTTGCTGAATGGACAG
  AGGAAGAATGTCAAAGATAGGAACTCCCCAAAGCAAACTGCAGATCGTTCAAAATCTCAGAGGAGCAGGA
  AAGGTGAATACTGGGAGACCTTCGAAAGCACTTGGAAGGGTTCAGTTAATGGAACGAAGATGCCAACTCC
  TAGGAAAGATAGTTGTAATGTATCTAAACGTGCCTGTCCCCTACAGCACAGAAGTGCACAGAAAAGCATT
  CTTCAAATGTTTCAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210589 representing NM_001127597
  Red=Cloning site Green=Tags(s)
  
  MKMAAPKNSIPSLAECQCGICMEILVEPVTLPCNHTLCNPCFQSTVEKANLCCPFCRRRVSSWTRYHTRR
  NSLVNTDLWEIIQKHYAKECKLRISGQESKEIVDEYQPVRLLSKPGELRREYEEEISKVEAERQASKEEE
  NKASEEYIQRLLAEEEEEEKRRTERRRSEMEEQLRGDEELARRLSTSINSNYERNILASPLSSRKSDPVT
  NKSQKKNTNKQKNFGDIQRYLSPKSKPGTAWACKTEHGEDMCKSKETDSSDTKSPVLQDTDVEESMPTHS
  PQTCPETQGQGPEPLTEMPVPWLCARNAEQCLEGKAEAVSTNPDDSCIVNDGGPRAIVSNSKEAAVKPPT
  KIENEEYSVSGVTQLTGGNGVPTESRVYDLLVGKEISERENQESVFEEVMDPCFSAKRRKIFITSSLDQE
  ETEVNFTQKLIDLEHMLFERHKQEEQDRLLALQLQKEADKEKMVPNRQKGSPDQYQLRTSSPPDGLLNGQ
  RKNVKDRNSPKQTADRSKSQRSRKGEYWETFESTWKGSVNGTKMPTPRKDSCNVSKRACPLQHRSAQKSI
  LQMFQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127597
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127597.2, NP_001121069.2
• RefSeq Size: 3311 bp
• RefSeq ORF: 1701 bp
• Locus ID: 690043
• UniProt ID: B2RYR0
• Cytogenetics: 11q22
• MW: 64.7 kDa
• Gene Summary: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).[UniProtKB/Swiss-Prot Function]