Hnrnpu (NM_057139) Rat Tagged ORF Clone

CAT#: RR209966

Hnrnpu (Myc-DDK-tagged ORF) - Rat heterogeneous nuclear ribonucleoprotein U (Hnrnpu), (10 ug)

Product Images

Circular map for RR209966

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hnrnpu
• Synonyms: hnRNP U; Hnrpu; SAF-A; SN1; SP120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209966 representing NM_057139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTTCGCCTGTTAATGTCAAGAAGCTGAAGGTGTCGGAGCTGAAGGAGGAGCTCAAGAAGCGGC
  GCCTCTCAGACAAGGGCCTCAAGGCCGATCTCATGGATCGACTCCAGGCCGCGCTGGACAACGAGGCAGG
  AGGCCGCCCCGCCATGGAGCCCGGGAACGGCAGCCTCGACCTGGGTGGCGATGCGGCCGGGCGCTCGGGA
  GCAGGCCTAGAGCAGGAGGCCGCGGCCGGCGCCGAAGACGATGAGGAGGAGGAGGGCATTGCCGCTCTGG
  ACGGCGACCAGATGGAGCTGGGCGAGGAGAACGGGGCGGCGGGGGCGGCTGACGCGGGCGCGATGGAGGA
  GGAGGAGGCGGCGTCGGAAGACGAGAACGGCGACGACCAGGGCTTCCAGGAGGGGGAGGACGAGCTCGGC
  GACGAAGAGGAGGGCGCGGGCGACGAGAACGGCCACGGGGAGCAGCAGTCCCAACCGCCGGCGGCGGCGC
  AGCAGGCTTCTCAGCAGCGCGGGCCTGGCAAGGAGGCCGCGGGGAAAAGCAGCGGCCCCACCTCGCTCTT
  CGCGGTGACGGTGGCGCCGCCAGGGGCGAGGCAGGGCCAACAGCAGGCGGGAGGAGACGGCAAAACAGAA
  CAGAAAGCTGGAGATAAAAAGAGAGGTGTTAAAAGACCCCGGGAAGATCATGGCCGAGGATATTTTGAGT
  ACATTGAAGAAAACAAGTACAGCAGAGCCAAGTCTCCTCAGCCACCTGTTGAAGAAGAAGACGAACACTT
  CGATGACACAGTGGTTTGTCTTGATACTTATAATTGTGATCTGCATTTTAAAATATCAAGAGACCGTCTC
  AGTGCTTCTTCCCTTACTATGGAGAGTTTCGCTTTTCTTTGGGCTGGAGGAAGAGCTTCTTATGGTGTGT
  CAAAAGGCAAAGTCTGCTTTGAGATGAAGGTAACAGAGAAGATTCCAGTAAGACATTTATATACAAAAGA
  TATTGATATACATGAAGTTCGGATTGGCTGGTCACTAACCACAAGTGGAATGTTGCTTGGTGAAGAAGAA
  TTTTCTTATGGGTATTCTCTGAAAGGAATAAAAACCTGCAACTGTGAGACAGAAGATTATGGAGAGAAGT
  TTGATGAAAATGATGTGATTACGTGCTTTGCTAACTTTGAGACTGATGAAGTTGAACTCTCTTACGCAAA
  GAATGGACAAGATCTTGGTGTTGCCTTTAAGATTAGTAAGGAAGTTCTTGCTGACCGGCCATTATTCCCC
  CATGTTCTCTGCCATAACTGTGCAGTTGAGTTTAATTTTGGTCAAAAGGAAAAGCCATACTTTCCAATAC
  CTGAAGACTGTACTTTTATCCAAAATGTCCCATTAGAGGACCGAGTCAGAGGACCAAAAGGACCTGAAGA
  GAAGAAGGATTGTGAAGTTGTGATGATGATTGGCTTGCCAGGAGCTGGAAAAACTACCTGGGTTACTAAA
  CATGCAGCTGAAAACCCTGGGAAATATAACATTCTTGGAACAAACACTATTATGGACAAAATGATGGTGG
  CAGGCTTTAAGAAGCAAATGGCAGATACTGGAAAACTGAACACACTGTTGCAGAGAGCCCCACAGTGTCT
  TGGCAAGTTTATTGAGATTGCTGCCCGAAAGAAGCGAAATTTTATTCTGGATCAGACAAATGTGTCTGCT
  GCTGCCCAGAGGAGAAAAATGTGCCTGTTTGCAGGCTTCCAACGAAAAGCCGTGGTCGTTTGCCCAAAAG
  ACGAAGACTATAAGCAGAGGACACAGAAGAAAGCGGAAGTAGAGGGGAAAGACCTACCAGAGCATGCTGT
  CCTCAAGATGAAAGGAAACTTCACCCTTCCAGAGGTTGCAGAGTGTTTTGATGAAATAACCTATGTTGAA
  CTTCAGAAGGAGGAAGCCCAGAAGCTTTTGGAGCAATATAAAGAAGAAAGCAAAAAGGCACTGCCACCAG
  AAAAAAAGCAAAACACTGGCTCAAAGAAAAGCAATAAAAATAAGAGTGGCAAGAACCAGTTCAACAGAGG
  TGGTGGCCATAGAGGACGTGGAGGATTCAATATGCGTGGTGGCAATTTCAGAGGGGGAGCTCCTGGGAAT
  CGTGGTGGATATAATAGGAGAGGCAACATGCCCCAGAGAGGTGGTGGCGGCGGAAGTGGTGGAATTGGCT
  ATCCATACCCGCGTGGCCCTGTCTTTCCTGGCCGAGGTGGTTACTCAAACAGAGGAAATTACAACAGAGG
  TGGAATGCCCAACAGAGGGAACTATAACCAGAACTTCAGAGGACGAGGAAATAATCGTGGCTACAAAAAT
  CAATCTCAGGGCTACAACCAGTGGCAGCAGGGTCAATTCTGGGGTCAGAAGCCATGGAGTCAGCATTATC
  ACCAAGGATATTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209966 representing NM_057139
  Red=Cloning site Green=Tags(s)
  
  MSSSPVNVKKLKVSELKEELKKRRLSDKGLKADLMDRLQAALDNEAGGRPAMEPGNGSLDLGGDAAGRSG
  AGLEQEAAAGAEDDEEEEGIAALDGDQMELGEENGAAGAADAGAMEEEEAASEDENGDDQGFQEGEDELG
  DEEEGAGDENGHGEQQSQPPAAAQQASQQRGPGKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGKTE
  QKAGDKKRGVKRPREDHGRGYFEYIEENKYSRAKSPQPPVEEEDEHFDDTVVCLDTYNCDLHFKISRDRL
  SASSLTMESFAFLWAGGRASYGVSKGKVCFEMKVTEKIPVRHLYTKDIDIHEVRIGWSLTTSGMLLGEEE
  FSYGYSLKGIKTCNCETEDYGEKFDENDVITCFANFETDEVELSYAKNGQDLGVAFKISKEVLADRPLFP
  HVLCHNCAVEFNFGQKEKPYFPIPEDCTFIQNVPLEDRVRGPKGPEEKKDCEVVMMIGLPGAGKTTWVTK
  HAAENPGKYNILGTNTIMDKMMVAGFKKQMADTGKLNTLLQRAPQCLGKFIEIAARKKRNFILDQTNVSA
  AAQRRKMCLFAGFQRKAVVVCPKDEDYKQRTQKKAEVEGKDLPEHAVLKMKGNFTLPEVAECFDEITYVE
  LQKEEAQKLLEQYKEESKKALPPEKKQNTGSKKSNKNKSGKNQFNRGGGHRGRGGFNMRGGNFRGGAPGN
  RGGYNRRGNMPQRGGGGGSGGIGYPYPRGPVFPGRGGYSNRGNYNRGGMPNRGNYNQNFRGRGNNRGYKN
  QSQGYNQWQQGQFWGQKPWSQHYHQGYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057139
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057139.2, NP_476480.2
• RefSeq Size: 3380 bp
• RefSeq ORF: 2397 bp
• Locus ID: 117280
• UniProt ID: Q6IMY8
• Cytogenetics: 13q25
• MW: 87.7 kDa
• Gene Summary: may be involved in glutamate transport and synaptogenesis [RGD, Feb 2006]