Mthfd2 (NM_001109398) Rat Tagged ORF Clone

CAT#: RR209354

Mthfd2 (Myc-DDK-tagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR209354

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mthfd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209354 representing NM_001109398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTGTTTCCTTGTCTGCGCTGGCAGTGCGGTTGTTGCGCCCCACGCACGGCTGCCACTCCCGCC
  TACAGTCCTTCCACCTGGCGGCAGTACGAAATGAAGCCGTGGTCATTTCTGGAAGGAAGCTGGCCCAGCA
  GATTAAGCAAGAAGTACGGCAGGAGGTGGAAGAGTGGGTGGCCTCGGGCAACAAGCGGCCACACCTCAGT
  GTCATTCTGGTTGGCGACAATCCTGCCAGTCACTCCTATGTTCTCAACAAAACCAGGGCAGCAGCTGAAG
  TGGGAATCAACAGTGAGACAATTGTGAAACCAGCCTCAGTGTCAGAGGAAGAGCTGTTGAATTCAATCAG
  GACATTGAATGACGATGAGAATGTAGATGGCCTCCTTGTTCAGCTGCCACTCCCAGAGCACATTGACGAG
  AGAAAGATCTGCAATGCTGTTTCTCCTGACAAGGATGTTGATGGCTTTCATGTCATCAACGTTGGGCGCA
  TGTGTTTGGACCAGTATTCCATGCTGCCAGCAACTCCATGGGGCGTGTGGGAGATACTCAAGCGAACAGG
  CATTCCAACCTTAGGGAAGAATGTGGTAGTGGCTGGCAGGTCCAAAAACGTTGGGATGCCAATTGCAATG
  CTGCTGCACACGGATGGGGCACACGAGCGGCCTGGGGGTGATGCCACAGTCACAATATCTCATCGGCACA
  CTCCCAAAGAACAGCTGAAGAAGCACACGATCCTTGCGGACATTGTGATATCTGCTGCTGGCATTCCAAA
  CCTCATCACAGCTGACATGATCAAGGAAGGAGCAACAGTCATCGACGTGGGGATAAACAGAGTTCAAGAT
  CCTGTCACCGCAAAGCCCAAGTTGGTTGGAGATGTGGATTTTGAAGGAGTCAAGAAGAAAGCTGGTTACA
  TCACTCCTGTCCCTGGGGGTGTTGGTCCCATGACAGTGGCCATGCTCATGAAGAACACCATTATTGCTGC
  AAAGAAAGTGCTCAGGCCAGAGGAGCTGGAAGTGTTGAAGTCCACACAGCGCGGAGTTGCCACCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209354 representing NM_001109398
  Red=Cloning site Green=Tags(s)
  
  MVSVSLSALAVRLLRPTHGCHSRLQSFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLS
  VILVGDNPASHSYVLNKTRAAAEVGINSETIVKPASVSEEELLNSIRTLNDDENVDGLLVQLPLPEHIDE
  RKICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEILKRTGIPTLGKNVVVAGRSKNVGMPIAM
  LLHTDGAHERPGGDATVTISHRHTPKEQLKKHTILADIVISAAGIPNLITADMIKEGATVIDVGINRVQD
  PVTAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRPEELEVLKSTQRGVATN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109398
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109398.1, NP_001102868.1
• RefSeq Size: 2061 bp
• RefSeq ORF: 1050 bp
• Locus ID: 680308
• Cytogenetics: 4q34
• MW: 37.7 kDa