Synrg (NM_053419) Rat Tagged ORF Clone

CAT#: RR209031

Synrg (Myc-DDK-tagged ORF) - Rat AP1 gamma subunit binding protein 1 (Ap1gbp1), (10 ug)

Product Images

Circular map for RR209031

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Synrg
• Synonyms: Ap1gbp1; Syng
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209031 representing NM_053419
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCGGCCGGGAGCCGGAGCCAGCGGCGCGGCGGGGGCGGGCACAGGGCCCGGCGGGGCAGGCA
  GCTTCATGTTTCCTGTCGCAGGTGGCATGAGACCCCCTCAAGGCCTGATTCCAATGCAGCAGCAAGGATT
  TCCTATGGTCTCTGTCATGCAGCCCAATATGCAAGGCATGATGGGAATGAATTACAGCTCTCAGATGTCC
  CAGGGACCCATTGCAATGCAGGCAGGAATACCAATGGGGCCAATGCCAGCAGCGGGTGTACCTTTCCTGG
  GTCAGCCACCTTTTCTGGGCATGCGTCCTGCAGCCCCACAGTACACTCCAGACATGCAGAAGCAGTTTGC
  GGAAGAGCAACAAAAACGATTTGAACAACAACAAAAGCTCTTAGAAGAAGAAAGAAAACGACGTCAGTTT
  GAAGAGCAAAAACAAAAGCTCAGACTTCTGAGTAGCGTGAAACCCAAGACAGGAGAGAAGAACAGAGACG
  ACGCTTTGGAAGCCATCAAAGGAAACTTGGATGGGTTTTCCAGAGATGCTAAGATGCACCCCACTCCCGC
  ATCTCACCCAAAGAAACCAGACTGCCCCACATCATCTCATTCTACTAAAACTGTCTCCCCGTCATCTGCC
  TTTCTTGGTGAAGATGAATTCAGTGGCTTTATGCAGGGGCCTGTTGAGCTTCCCACTTGTGGGCCTTCTT
  CCACAGCCCAGCCCTTTCAGTCTTTCCTCCCCAGCACCCCACTCGGCCAGTTACACACACAGAAGGCTGG
  GGCACAGCCTCTACCTCCAGGTCAGGCTCCAGTGTCTTTTGCCGTACATGGTGTCCATGGGCAAATTCCT
  TGTTTATCTGCTGCTTCAGCTTCCCACAGTATGCAGAAAGCAGGCCCTTCCTTGGAAGAGAAGCTCCTAG
  TATCTTGTGATATAAGTGCATCTGGGCAGGAACACATTAAATTAAGCTCTCCTGAAGCTGGGCACAGAGC
  CGTAGTCCCAGGTTCCAGTAAGAACTCTCCCGGTTTAATGGCCCATAACGGGGGTGCTGTAGATGGATGT
  GTGAGTGGTCCCACCACTGCAGTCGCAGAAAAGACTTCAGATCAAAACCTGTCAAAGGAAGAGAGTGGTG
  TGGGCGTGTTTCCCTCTCAGGATCCTGTTCAGCCCAGAATGCCTCCTTGGATTTACAATGAGAGTTTGGT
  TCCAGATGCTTATAAGAAAATTTTAGAAACCACAATGACTCCAACCGGAATAGACACTGCTAAACTTTAT
  CCCATTCTGATGTCATCTGGACTTCCCAGGGAAACTCTTGGACAGATATGGGCCTTAGCTAATCGAACTA
  CGCCTGGCAAGCTCACTAAGGAGGAGCTTTACACCGTTCTCGCCATGGTAGCAGTAACACAGAGAGGTGT
  TCCTGCCATGAGCCCTGATACTTTGAACCAGTTCCCTGCGGCTCCCATTCCTACCTTAAGCGGCTTTCCT
  ATGACTCTGCCTACCCCAGTGAGCCAGCCAACAGCGATGACCTCTGGCCCCGCAGGCTCCATACCCCTCA
  GCCTTGGACAGCCCATCATGGGCATTAACCTTGTTGGACCAGTGGGTGGAGCTGCAGCGCCAACTTCCAG
  TGGCTTCATGCCAGCCTACCCCTCAAACCAGGTGGGAAAGACAGAAGAAGATGACTTCCAGGATTTTCAA
  GATGCTTCGAAGTCAGGATCCATCGATGATTCTTTCACTGACTTCCAAGAGGTGCCTGCGTCCTCAAAAA
  CCAGTAATTCCCAGCATGGAAACAGTGCTCCCTCACTCTTGATCCCACTTCCTGGAACTAAAGCCTCCAC
  AGACAAATATGCAGTGTTTAAAGGGATTTCTGCTGAGAAGCCCTCTGAAAACCCTGCTTCATTTGGAGAG
  TCCGGTGATAAATACAGTGCTTTCAGGGAACTCGAGCCAACAGCAGACAGTAAGCCTTTAGGAGAGAGCT
  TTGCGGAGTTCAGATCCACAGGAACCGATGATGGCTTCACTGACTTCAAGACTGCTGATAGTGTGTCACC
  CCTAGAGCCACCAACAAAAGACTCTTTTCCATCGGCCTTTGCCTCAGGAGCTGCACAGCAGACACAGACA
  CAGGTGAAAACCCCTCTGAACTTAGCAGACCTAGATATGTTCTCCTCAGTGGATTGCAGTGGGGAGAAAC
  CAGTGCCCTTTTCAGCTGCATTTAGCACCTCAAAGTCAGTTTCCTCAAGACCTCAGCCGGCGGGTTCTGC
  AGCAGCCCCAGCATCACTGGCATCCACTAAAGCGTCCAGTTTGGCAGATGATTTTGGAGAGTTCAACCTC
  TTTGGGGAATATTCGAACCCTGCGTCTGTTGGGGAGCAGGACGACTTTGCAGATTTCATGGCTTTCGGTA
  ACAGCTCCATCCCCTCTGAGCCAAAAGCAGATGACAAGTATGAAGCCCTCAGAGAAGAAGGGAGCCCCGG
  CGCCTTGTCCACCAGCACTGTGGAGGGTGCACACAACCCACCTGTTTCGTCATCCAAGTACGACGTCTTC
  AAACAGCTTTCCCTAGAGGGAGCTGGACTGGCCATCGAGGAGTTCAAAGAGAACACTCCTTCCACAAAAA
  GTGACGGTGACTTTGCTGACTTCCACTCCAGTAAATTCTCATCCACGAGCTCAGACAAATCCCTGGGAGA
  GAAAGCAGTGGCTTTCAGACATGCCAAAGAAGACTCCGCCTCGGTGAAGTCTTTAGACCTCCCCTCCATC
  GGTGGCAGCAGTGTTGGCAAGGAGGACTCTGAAGATGCGCTTTCTGTTCAGTTTGACATGAAGTTGGCTG
  ATGTAGGAGGAGATCTTAAGCATGTCATGTCTGATAGCTCTTTGGATTTACCAACAGTTAGTGGCCAACA
  TCCTCCTGCCGCAGGATCAGCCTTGGCCAGTGAGGACGCTCTTCCAGAGACTCCCTTCCCAGCTTTTGCC
  AGCTTTAAAGACATGATGCCCCAGACCACTGAGCAGAAAGAGTATGAAAGTGGGGACTTCCAGGATTTCA
  CAAGGCAGGACATGCCCATGGTGGACCGGAGCCAAGAGAACACATGTCCCAGCCCAGCTTCCAGTGTGGC
  CTCGCACGAAACCCCCAAGGAGGGTGCAGACGACTTTGGAGAATTTCAAAGTGAAAAGCCCAAAATCAGC
  AAGTTTGACTTTTTAGTAGCTAATTCACAGAGCAAAATGAAGTCTAGTGAAGAAATGATCAAAAGTGAGC
  TGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGAGCTTGAGCCTTGGGGATAAAGAAATAAG
  CAGGTCCTCTCCTTCCCCGGCCCTGGAGCAGCCCTTCAGAGACCGGTCCAACACTCTGAGTGAGAGAGCT
  GCACTGCCCGTCATCCGAGACAAGTACAAGGATCTGACTGGCGAAGTGGAGGAAAATGAGAGATACGCGT
  ATGAGTGGCAGAGATGCCTGGGGAGCGCCCTGGATGTCATTAAGAAGGCAAACGACACCTTAAACGGCAT
  TAGCAGCAGTGCTGTCTGCACAGAAGTTATCCAGTCAGCCCAAGGCATGGAGTATCTCCTAGGCGTGGTG
  GAAGTGTACCGAGTAACCAAGCGCGTGGAGCTGGGGATCAAAGCCACTGCTGTGTGTAGTGAGAAACTCC
  AGCAGTTGCTGAAGGACATCGATAAAGTGTGGAACAACTTAATCGGCTTCATGTCACTTACCACACTCAC
  GCCAGATGAGAATTCACTGGATTTTTCCTCCTGTATGTTACGGCCTGGGATCAAAAACGCCCAGGAGCTG
  GCTTGTGGAGTGTGTCTCCTAAATGTGGACTCCAGAAGCCGGAAAGAAGAGACACCTGCAGAAGAACAGC
  CTAAAAAAGCATTCAACTCAGAAACAGACAGTTTCAAGCTGGCCTATGGGGGACATCAGTACCACGCCAG
  CTGTGCCAACTTCTGGATCAACTGTGTTGAACCCAAGCCTCCCGGCCTCCTCTTGCCAGATCTGCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209031 representing NM_053419
  Red=Cloning site Green=Tags(s)
  
  MALRPGAGASGAAGAGTGPGGAGSFMFPVAGGMRPPQGLIPMQQQGFPMVSVMQPNMQGMMGMNYSSQMS
  QGPIAMQAGIPMGPMPAAGVPFLGQPPFLGMRPAAPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQF
  EEQKQKLRLLSSVKPKTGEKNRDDALEAIKGNLDGFSRDAKMHPTPASHPKKPDCPTSSHSTKTVSPSSA
  FLGEDEFSGFMQGPVELPTCGPSSTAQPFQSFLPSTPLGQLHTQKAGAQPLPPGQAPVSFAVHGVHGQIP
  CLSAASASHSMQKAGPSLEEKLLVSCDISASGQEHIKLSSPEAGHRAVVPGSSKNSPGLMAHNGGAVDGC
  VSGPTTAVAEKTSDQNLSKEESGVGVFPSQDPVQPRMPPWIYNESLVPDAYKKILETTMTPTGIDTAKLY
  PILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMVAVTQRGVPAMSPDTLNQFPAAPIPTLSGFP
  MTLPTPVSQPTAMTSGPAGSIPLSLGQPIMGINLVGPVGGAAAPTSSGFMPAYPSNQVGKTEEDDFQDFQ
  DASKSGSIDDSFTDFQEVPASSKTSNSQHGNSAPSLLIPLPGTKASTDKYAVFKGISAEKPSENPASFGE
  SGDKYSAFRELEPTADSKPLGESFAEFRSTGTDDGFTDFKTADSVSPLEPPTKDSFPSAFASGAAQQTQT
  QVKTPLNLADLDMFSSVDCSGEKPVPFSAAFSTSKSVSSRPQPAGSAAAPASLASTKASSLADDFGEFNL
  FGEYSNPASVGEQDDFADFMAFGNSSIPSEPKADDKYEALREEGSPGALSTSTVEGAHNPPVSSSKYDVF
  KQLSLEGAGLAIEEFKENTPSTKSDGDFADFHSSKFSSTSSDKSLGEKAVAFRHAKEDSASVKSLDLPSI
  GGSSVGKEDSEDALSVQFDMKLADVGGDLKHVMSDSSLDLPTVSGQHPPAAGSALASEDALPETPFPAFA
  SFKDMMPQTTEQKEYESGDFQDFTRQDMPMVDRSQENTCPSPASSVASHETPKEGADDFGEFQSEKPKIS
  KFDFLVANSQSKMKSSEEMIKSELATFDLSVQGSHKRSLSLGDKEISRSSPSPALEQPFRDRSNTLSERA
  ALPVIRDKYKDLTGEVEENERYAYEWQRCLGSALDVIKKANDTLNGISSSAVCTEVIQSAQGMEYLLGVV
  EVYRVTKRVELGIKATAVCSEKLQQLLKDIDKVWNNLIGFMSLTTLTPDENSLDFSSCMLRPGIKNAQEL
  ACGVCLLNVDSRSRKEETPAEEQPKKAFNSETDSFKLAYGGHQYHASCANFWINCVEPKPPGLLLPDLL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053419
• ORF Size: 3987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053419.1, NP_445871.1
• RefSeq Size: 3990 bp
• RefSeq ORF: 3990 bp
• Locus ID: 84479
• UniProt ID: Q9JKC9
• Cytogenetics: 10q26
• MW: 141.4 kDa
• Gene Summary: may link the AP1 adaptor complex to other proteins [RGD, Feb 2006]