Zfp574 (NM_001024258) Rat Tagged ORF Clone

CAT#: RR209018

Zfp574 (Myc-DDK-tagged ORF) - Rat zinc finger protein 574 (Znf574), (10 ug)

Product Images

Circular map for RR209018

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp574
• Synonyms: Znf574
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209018 representing NM_001024258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAAGAGAGTGAAGAAACGGTCTTGTATATTGAGCACCGCTATGTCTGCTCTGAGTGCAACCAGC
  TCTATGGATCCTTGGAGGAGGTGCTTGTGCACCAGAACTCCCATGTGCCCCAGCAGCACTTTGAGCTGGT
  GGGCGTGGCTGACCCTGGAGTCACAGTGGCCACAGAGGCCACTTCAGGTACAGGCCTATATCAGACCCTC
  ATACAGGAGAGCCAGTACCAGTGTCTCGAGTGTGGGCAGCTTCTCCTGTCACCCAGCCAGCTCCTGGAGC
  ACCAAGAGCTGCACCTGAAGATGATGGCACCCCAGGAGGCAGTACCAGCTGAGCCACCACCCAAGGTGCC
  CCCCTTGAGCTCCAGCACCATCCACTATGAGTGTGTGGACTGTAAGGCTCTGTTCGCCAGCCAAGAGATG
  TGGCTGAGCCATCGGCAGACGCATCTCAGGGCCACACCCAACAAGGCTCCTGCCCCTGTTGTCTTGGGGT
  CTCCAGTTGTCTTGGGCCCCCCTGTGGGCCAGGCCCGAGTAGCTGTGGAACATTCGTACCGAAAAGCAGA
  AGAGGGTGGGGAGGGGGCAGCTGTCCCATCTGTGGCTGCTGCCACTGAGATGGTGACAGAGGTGGAACTG
  CTCCTCTACAAATGCTCTGAGTGCTCCCAGCTCTTCCAGATGCCAGCTGACTTCCTGGAACATCAGGCCA
  CCCACTTCCCTGCTCCTGTCCCAGAGGCCGAGGAGCCTGCCACCCAGCAGGAAACTCTGGTCCCCTCACC
  CACTGAGGCGGCAGTATCTCAGCCTGACCCCCTGCCAGCCTCTGATCACAGTTATGAGCTGCGTAACGAG
  CTGAGAAATGGGGAGGCCATGGGGCGAGATCGCCGTGGGCGGAAGCCCCGGAGGAGCAGCAGTGGAGAGT
  CGGGTGCCACCCAGGAACTGTTTTGCTCAGCCTGTGACCAGATCTTTCTCTCACCCCACCAGCTGCAACA
  GCACCTTCGGAGTCACCGGGAGGGTGTTTTTAAGTGTCCCCTGTGCAGTCGTGTCTTCCCCAGCCCTTCT
  AGTCTGGACCAACACCTTGGTGACCATAGCAGTGAGTCACATTTTCTGTGTGTGGACTGTGGCCTAGCTT
  TTGGCACAGAAGCTCTCCTCCTGGCCCACCGTCGAGCCCATACCCCGAACCCTCTGCATTCATGTCCCTG
  TGGGAAGACCTTTGTTAACCTCACCAAGTTCCTTTATCACCGGCGTACCCATGGAGCAGGAGGTGTCCCT
  TTGCCCACAACACCAGTTCCACCAGAGGAGCCTGCTATCAGCTTTCCTGAGCCGGCCCCTGCAGAGACTG
  GAGAGCTAGAGGCCCCGGAGCTCCCTGTATCTGAGGAGAGTTCAGCTGAGCCTGCAGCTCCAGGCACTTA
  CCGCTGCCTCCTGTGTAGCCGGGAGTTTAGTAAGGCATTGCAGCTGACCCGGCACCAGCGTTTTGTGCAC
  CGGCTGGAGCGGCGCCATAAATGCAGCATTTGTGGCAAGATGTTCAAGAAGAAGTCTCACGTGCGGAACC
  ATCTGCGTACACACACAGGGGAACGGCCCTTCCCCTGCCCTGACTGCTCTAAACCTTTCAACTCACCAGC
  CAACCTGGCACGGCACCGGCTGACACACACAGGGGAGCGGCCCTATCGCTGTGGGGACTGTGGCAAGGCT
  TTCACTCAGAGCTCTACTTTGAGGCAGCATCGCCTGGTGCATGCCCAGCATTTCCCCTACCGCTGCCAGG
  AGTGTGGAGTACGTTTTCACCGCCCTTACCGCCTGCTCATGCACCGCTACCACCACACAGGCGAGTACCC
  CTACAAATGTCGGGAGTGCCCCCGCTCCTTCTTGCTGCGCCGGCTGCTAGAGGTCCACCAGCTTGTGGTC
  CATGCTGGGCGACAGCCCCACCGCTGCCCGTCCTGTGGGGCTGCCTTCCCCTCGTCACTACGGCTTCGTG
  AGCATCGCTGTGCGGCTGCTGCCGCCCAGGCCCCACGGCGCTTTGAGTGTGGCACCTGCGGCAAGAAAGT
  GGGCTCTGCTGCTCGCCTGCAGGCACATGAAGCTGCCCACGCTGCTGCAGGTCCTGGAGAGGTCTTGGCT
  AAGGAGCCTCCGGCTCCCCGGGCCGCAAGGGCCACTCGCACACCAGTTGCTCCCTCTCCAACAGCCCTTG
  GTGGTACAACCTCTGCTGCCCCTGCAGCCCCTGCCCGGCGCCGGGGTCTGGAATGCAGTGAGTGCAAGAA
  GCTCTTCAGCACGGAGACATCACTGCAGGTCCATCGGCGCATCCACACAGGCGAGCGGCCATACCCGTGC
  CCAGACTGTGGCAAGGCCTTCCGCCAGAGTACCCATCTGAAAGACCACAGGCGCTTGCACACAGGCGAGC
  GGCCCTTTGCTTGCGAAGTGTGTGGCAAGGCCTTTGCCATCTCCATGCGCCTGGCAGAACATCGACGCAT
  CCACACGGGTGAACGACCCTACTCCTGCCCTGACTGTGGCAAGAGCTACCGCTCCTTCTCCAATCTCTGG
  AAACACCGCAAGACTCACCAGCAGCAGCATCAGGCAGCAGTGCGGCAGCAGCTGGCAGAGGCCGAGGCTG
  CTGTGGGCCTGGCTGTGATGGAAACTGCAGTGGAGGCTCTGCCCCTTGTGGAGGCCATTGAGATCTACCC
  TCTAGCTGAAGCGGATGGGGTCCAGATCAGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209018 representing NM_001024258
  Red=Cloning site Green=Tags(s)
  
  MTEESEETVLYIEHRYVCSECNQLYGSLEEVLVHQNSHVPQQHFELVGVADPGVTVATEATSGTGLYQTL
  IQESQYQCLECGQLLLSPSQLLEHQELHLKMMAPQEAVPAEPPPKVPPLSSSTIHYECVDCKALFASQEM
  WLSHRQTHLRATPNKAPAPVVLGSPVVLGPPVGQARVAVEHSYRKAEEGGEGAAVPSVAAATEMVTEVEL
  LLYKCSECSQLFQMPADFLEHQATHFPAPVPEAEEPATQQETLVPSPTEAAVSQPDPLPASDHSYELRNE
  LRNGEAMGRDRRGRKPRRSSSGESGATQELFCSACDQIFLSPHQLQQHLRSHREGVFKCPLCSRVFPSPS
  SLDQHLGDHSSESHFLCVDCGLAFGTEALLLAHRRAHTPNPLHSCPCGKTFVNLTKFLYHRRTHGAGGVP
  LPTTPVPPEEPAISFPEPAPAETGELEAPELPVSEESSAEPAAPGTYRCLLCSREFSKALQLTRHQRFVH
  RLERRHKCSICGKMFKKKSHVRNHLRTHTGERPFPCPDCSKPFNSPANLARHRLTHTGERPYRCGDCGKA
  FTQSSTLRQHRLVHAQHFPYRCQECGVRFHRPYRLLMHRYHHTGEYPYKCRECPRSFLLRRLLEVHQLVV
  HAGRQPHRCPSCGAAFPSSLRLREHRCAAAAAQAPRRFECGTCGKKVGSAARLQAHEAAHAAAGPGEVLA
  KEPPAPRAARATRTPVAPSPTALGGTTSAAPAAPARRRGLECSECKKLFSTETSLQVHRRIHTGERPYPC
  PDCGKAFRQSTHLKDHRRLHTGERPFACEVCGKAFAISMRLAEHRRIHTGERPYSCPDCGKSYRSFSNLW
  KHRKTHQQQHQAAVRQQLAEAEAAVGLAVMETAVEALPLVEAIEIYPLAEADGVQISG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024258
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024258.1, NP_001019429.1
• RefSeq Size: 3097 bp
• RefSeq ORF: 2697 bp
• Locus ID: 308434
• UniProt ID: Q504L7
• Cytogenetics: 1q21
• MW: 99.4 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]