Tek (NM_001105737) Rat Tagged ORF Clone
CAT#: RR208604
- TrueORF®
Tek (Myc-DDK-tagged ORF) - Rat TEK tyrosine kinase, endothelial (Tek), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001105737" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tek |
Synonyms | Tie-2; Tie2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208604 representing NM_001105737
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCTTTAGCCGGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTATGGACTAGTAGAAGGAGCCA TGGACCTGATCTTGATCAATTCCCTACCTCTTGTGTCTGATGCCGAAACATCCCTCACCTGCATTGCTTC TGGGTGGCACCCCCACGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAAGAT CCACTGGAAGTTACTCAAGATGTGACCAGAGAATGGGCGAAAAAGGTTGTTTGGAAGAGAGAAAAGGCCA GTAAGATTAATGGTGCTTATTTCTGTGAAGGTCGAGTTCGAGGACAGGCTATAAGGATACGAACCATGAA GATGCGTCAACAAGCTTCCTTTCTACCTGCTACTCTAACTATGACCGTGGACAGGGGAGATAATGTGAAC ATATCCTTCAAAAAGGTGTTAATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCCTTCATTCATT CAGTGCCCCGGCATGAAGTTCCTGATATTTTAGAAGTGCATCTGCCTCACGCTCAGCCCCAGGATGCTGG TGTGTACTCGGCCAGGTACATAGGAGGAAACCTGTTCACCTCGGCCTTCACCAGGCTGATTGTCCGGAGA TGTGAAGCTCAGAAGTGGGGACCCGACTGTAACCGTCCTTGTACTACTTGCAAGAACAATGGTGTCTGCC ATGAAGATACTGGGGAGTGCATTTGCCCTCCTGGGTTTATGGGGAGGACATGTGAAAAGGCTTGTGAGCC ACACACATTTGGCAGGACCTGTAAGGAACGGTGTAGTGGATCAGAGGGATGCAAGTCATATGTGTTCTGT CTGCCAGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAGGGGGCTGCAGTGCAATGAAGCATGTCCAT ACGGTCACTATGGGCCAGACTGTAAGCTCAGGTGCCACTGTACCAATGAAGAGATGTGTGACCGCTTCCA AGGATGCCTCTGCTCTCAAGGATGGCAAGGGCTGCAGTGTGAGAAAGAAGGCAGGCCAAGGATGACTCCA CAGATAGAGGATTTGCCAGATCACATAGAAGTAAACAGTGGGAAATTTAACCCGATCTGCAAGGCCTCTG GCTGGCCACTACCTACTAGTGAAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCAACCAAATGA CTTCAACCATACAGATCATTTCTCAGTGGCCATATTCACTGTCAACCGGATCTTACCTCCTGACTCAGGA GTTTGGGTCTGCAGTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCTTTCAACATTTCTGTCAAAGTTC TTCCAGAGCCCCTGCATGCCCCAAATGTTATTGACACTGGACACAACTTTGCTATCATCAACATCAGCTC TGAGCCTTACTTTGGGGATGGACCGATCAAATCCAAGAAGCTCTTCTATAAACCTGTCAATCAGGCTTGG AAATACATTCAAGTGATGAATGAGATTGTCACACTCAACTACCTGGAGCCTCGGACTGACTACGAGCTGT GTGTACAGCTGGTCCGTCCTGGAGAGGGTGGAGAAGGACATCCTGGACCTGTGAGAAGATTCACAACAGC GTCTATCGGACTCCCTCCTCCAAGAGGTCTCAGTCTCCTACCCAAAAGCCAGACAGCTCTGAATTTGACT TGGCAACCGATATTTACAAGCTCAGAAGATGAATTTTATGTGGAAGTTGAGAGGTGGTCCCAGCAAACAA GAAGTGATCAGCAGAACATCAAAGTGCCTGGGAACCTGACTTCCGTGCTGCTGAACAACTTACTCCCCAG GGAGCAGTACAGCGTCCGAGCTAGAGTCAACACCAAGGCCCAGGGGGAGTGGAGTGAAGAACTCAGGGCC TGGACCCTTAGTGACATTCTCCCTCCTCAGCCAGAAAACATCAAGATCACCAACATCACTGATTACACAG CTCTGGTTTCTTGGACAATCGTGGACGGCTATTCGATTTCTTCCATCATCATCCGGTATAAGGTTCAGGG CAAAAATGAAGACCAGCACATTGACGTGAAGATCAAGAATGCCACCATCACTCAATACCAGCTCAAGGGC CTAGAGCCAGAGACTACATACCATGTGGATATTTTTGCTGAGAACAACATAGGATCAAGCAACCCAGCCT TTTCCCAAGAAATTAGGACACTTCCAGCCCCTAAAGACCTTGGAGGGGGAAAGATGCTACTTATAGCCAT TCTTGGGTCGGCTGGAATGACTTGCATCACCGTGCTATTGGCGTTTCTGATTATGTTGCAACTGAAGAGA GCAAATGTCCAAAGAAGAATGGCCCAGGCCTTCCAGAACGTGAGAGAAGAACCAGCTGTTCAGTTCAACT CAGGAACTCTGGCCCTAAACAGGAAGGCCAAAAACAATCCGGATCCCACAATTTATCCTGTGCTTGACTG GAATGACATCAAGTTCCAAGATGTGATTGGAGAGGGCAACTTTGGCCAGGTTCTGAAGGCGCGCATCAAG AAGGATGGGTTACGGATGGACGCTGCCATCAAGAGGATGAAAGAATATGCCTCCAAAGATGATCACAGGG ACTTTGCAGGAGAACTGGAGGTTCTTTGTAAACTTGGACACCATCCGAACATCATTAATCTCTTGGGAGC ATGTGAACACAGAGGCTACTTATACCTGGCTATTGAGTATGCCCCACATGGAAACCTCCTGGACTTTCTG CGTAAGAGCCGAGTGCTAGAGACAGACCCTGCCTTTGCCATCGCCAACAGCACGGCTTCCACACTGTCCT CCCAGCAGCTTCTTCATTTTGCTGCAGATGTGGCCCGGGGGATGGACTACTTGAGCCAAAAACAGTTTAT CCACAGGGACCTGGCTGCCAGAAACATTTTAGTTGGCGAAAACTACATAGCCAAAATAGCAGATTTTGGA TTGTCACGAGGTCAAGAAGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGCTGGATGGCAATTG AGTCTCTGAACTATAGTGTCTATACAACCAACAGTGATGTATGGTCCTATGGTGTATTGCTCTGGGAGAT CGTTAGCTTAGGAGGCACTCCATACTGCGGCATGACATGTGCAGAACTCTATGAGAAGCTGCCCCAGGGC TACAGATTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAATGCTGGAGGGAGA AGCCTTATGAGAGACCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGCTGGAAGAACGAAAGAC ATACGTGAACACCACACTTTATGAGAAGTTTACCTACGCAGGAATTGACTGTTCTGCTGAAGAAGCAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208604 representing NM_001105737
Red=Cloning site Green=Tags(s) MDSLAGLVLCGVSLLLYGLVEGAMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQD PLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVN ISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRR CEAQKWGPDCNRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGSEGCKSYVFC LPDPYGCSCATGWRGLQCNEACPYGHYGPDCKLRCHCTNEEMCDRFQGCLCSQGWQGLQCEKEGRPRMTP QIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNHTDHFSVAIFTVNRILPPDSG VWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAW KYIQVMNEIVTLNYLEPRTDYELCVQLVRPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLT WQPIFTSSEDEFYVEVERWSQQTRSDQQNIKVPGNLTSVLLNNLLPREQYSVRARVNTKAQGEWSEELRA WTLSDILPPQPENIKITNITDYTALVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATITQYQLKG LEPETTYHVDIFAENNIGSSNPAFSQEIRTLPAPKDLGGGKMLLIAILGSAGMTCITVLLAFLIMLQLKR ANVQRRMAQAFQNVREEPAVQFNSGTLALNRKAKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIK KDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFL RKSRVLETDPAFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYIAKIADFG LSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQG YRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001105737 |
ORF Size | 3360 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001105737.1, NP_001099207.1 |
RefSeq Size | 4559 bp |
RefSeq ORF | 3363 bp |
Locus ID | 89804 |
Cytogenetics | 5q33 |
MW | 125.8 kDa |
Gene Summary | mediates development of embryonic vasculature; plays a role in tumors and skin wounds [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208604 | Tek (untagged ORF) - Rat TEK tyrosine kinase, endothelial (Tek), (10 ug) |
USD 1,073.00 |
|
RR208604L3 | Lenti ORF clone of Tek (Myc-DDK-tagged ORF) - Rat TEK tyrosine kinase, endothelial (Tek), (10 ug) |
USD 1,372.00 |
|
RR208604L4 | Lenti ORF clone of Tek (mGFP-tagged ORF) - Rat TEK tyrosine kinase, endothelial (Tek), (10 ug) |
USD 1,372.00 |
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