Sema5a (NM_001107659) Rat Tagged ORF Clone

CAT#: RR208579

Sema5a (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)

Product Images

Circular map for RR208579

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema5a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208579 representing NM_001107659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTCGGCCTGAGA
  CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCCGTCGTCTCCTACAAAGAAATTGGCCCCTGGTT
  ACGGGAATTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT
  GTCGTAGGAGCAAGAAACTATCTCTTCAGACTACAGCTCGAGGATCTGTCTCTCATCCAGGCTGTGCAGT
  GGGAGTGTGATGAAGCCACGAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA
  CATACGAGTGCTCTTAGTGGGCGGGGACCGGCTCTTCACCTGTGGGACCAATGCCTTCACACCCGTCTGT
  ACCATCCGCTCCTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCTCGATGTCCCTATA
  GTCCCCAGCACAATTCTACAGCTCTACTCACTGCCAGTGGCGAGCTCTATGCTGCAACAGCCATGGATTT
  CCCAGGGCGCGATCCAGCCATTTATCGAAGTCTGGGCACTTTACCTCCTCTTCGAACTGCACAGTACAAC
  TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGGAACTTCACCTACTTCTTCTTCC
  GAGAAAATGCCGTGGAACACGACTGTGGGAAGACTGTGTTCTCCAGGGCAGCCAGGGTCTGCAAGAATGA
  CATTGGAGGACGATTTCTTCTGGAGGATACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCTCGG
  CCTGGTGAGGTGCCGTTCTACTACAACGAGCTTCAAAGCACCTTCTTCCTGCCGGAGCTGGATCTGATCT
  ACGGCATCTTCACCACCAATGTAAACAGCATTGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT
  CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAGAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC
  CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTATGTAAACCTGACGGAAAGAAACCTAC
  AGGATGCTCAGAAGTTCATCCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCTTCCTTCATGGA
  AGACAACAGCCGTTTCTCCCATGTGGCTGTCGATGTTGTGCAAGGCAGGGACACCCTTGTCCATATCATC
  TATCTGGCCACAGATTATGGCACCATTAAAAAAGTGCGGGCTCCGCTGAGTCAGAGCTCGGGCAGCTGTT
  TGCTAGAAGAGATTGAGCTTTTTCCAGAGAGGAAGAGTGAGCCCATCAGGAGCCTAAAGATCCTCCACAG
  CCAGAGTGTCCTATTTGTGGGGCTACAGGAGCATGTGGTCAAGATCCCCCTGAAGAGATGCCATTTTCAT
  CAAACACGTGGTGCCTGCATTGGTGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAGTGCA
  CCAGCCTGGAGGAGAGCCTGAGCATGACCCAGTGGGATCAGAGCGTCCCCACCTGTCCGACCAGAAATCT
  CACCGTAGATGGGAGCTTTGGCCCGTGGTCTCCATGGACACCCTGTACTCACACTGATGGCACTGCTGTG
  GGATCCTGCCTCTGCCGGTCCCGATCCTGTGACAGCCCAGCTCCACAGTGTGGCGGCTGGCAGTGTGAGG
  GCCCTAGAATGGAGATCACCAACTGTTCCAGGAATGGAGGCTGGACTCCCTGGACCTCCTGGTCTCCCTG
  CAGCACCACTTGTGGCATTGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCAACCCTACGCCCAGGCATGGA
  GGGCGTGTGTGCGTGGGCCAGAACAGGGAGGAAAGATACTGCAATGAACATTTACTCTGCCCACCACACG
  TGTTCTGGACAGGCTGGGGACCTTGGGAACGCTGCACAGCCCAGTGCGGGGGTGGCATTCAAGCTCGCCG
  TAGGACCTGTGAAAATGGGCCTGACTGTGCAGGATGCAATGTGGAATACCAGCCTTGTAACACCAATGCA
  TGCCCAGAGTTGAAGAAGACCACACCATGGACCCCCTGGACACCTGTCAACATCTCTGACAATGGGGGCC
  ACTATGAGCAGCGGTTCCGCTACACCTGTAAAGCTCGCCTGCCAGATCCAAACTTATTGGAAGTAGGAAG
  ACAAAGGATAGAAATGCGGTACTGTTCTAGTGATGGGACCAGTGGCTGCTCCACAGATGGACTTTCTGGA
  GACTTTCTAAGAGCTGGGAGATACTCTGCCCATACAGTCAATGGGGCATGGTCAGCCTGGACTTCCTGGT
  CACAGTGCAGCAGAGACTGCAGCAGGGGCATTCGAAACAGGAAGCGTGTTTGCAACAACCCAGAGCCCAA
  GTATGGAGGTATGCCATGCCTTGGTCCATCACTGGAGTTCCAGGAATGCAACATTTTACCCTGTCCAGTG
  GATGGTGTGTGGTCTTGCTGGTCATCCTGGTCTAAATGCTCAGCAACTTGTGGGGGTGGACACTACATGA
  GAACCCGTTCGTGTACGAATCCAGCCCCAGCATACGGAGGGGACATCTGCCTGGGACTGCACACGGAAGA
  GGCCCTCTGTAACACACAGACCTGCCCAGAGAACTGGTCAGAGTGGTCAGAGTGGTCTGTGTGTGATGCA
  TCTGGTACCCAGGTCCGTACTCGGCAGTGCATCCTTCTGTTTCCTGTGGGCAGCCAGTGTTCTGGAAATA
  CTACAGAGAGCCGGCCTTGTGTATTTGACTCTAATTTCATCCCAGAAGTGTCTGTGGCAAGATCCAGCAG
  CGTAGAAGAGAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATGGCTGTGGGACTTAGCAGTTCCATC
  CTCGGCTGCCTCCTTACACTGCTTGTCTACACCTACTGCCAGCGGTACCAGCAGCAGTCCCATGATGCAA
  CTGTCATCCATCCTGTTTCTCCCGCCGCCCTCAACAGCAGCATAACTAACCACATCAACAAACTGGATAA
  ATACGATTCCGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAGAGAAACAAATAC
  TTCAACCCACATCTCACTGGGAAGACCTACTCCAACGCCTACTTTACAGATCTCAACAATTATGATGAAT
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208579 representing NM_001107659
  Red=Cloning site Green=Tags(s)
  
  MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKEL
  VVGARNYLFRLQLEDLSLIQAVQWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVC
  TIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYN
  SKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSR
  PGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPN
  PNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGRDTLVHII
  YLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERKSEPIRSLKILHSQSVLFVGLQEHVVKIPLKRCHFH
  QTRGACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSVPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAV
  GSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHG
  GRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNA
  CPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSG
  DFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEFQECNILPCPV
  DGVWSCWSSWSKCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQTCPENWSEWSEWSVCDA
  SGTQVRTRQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSI
  LGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKY
  FNPHLTGKTYSNAYFTDLNNYDEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107659
• ORF Size: 3222 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107659.2, NP_001101129.1
• RefSeq Size: 10501 bp
• RefSeq ORF: 3225 bp
• Locus ID: 310207
• UniProt ID: D3ZTD8
• Cytogenetics: 2q22
• MW: 120.4 kDa
• Gene Summary: Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.[UniProtKB/Swiss-Prot Function]