Prkg1 (NM_001105731) Rat Tagged ORF Clone
CAT#: RR208372
- TrueORF®
Prkg1 (Myc-DDK-tagged ORF) - Rat protein kinase, cGMP-dependent, type 1 (Prkg1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001105731" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Prkg1 |
Synonyms | cGk1; Pkgi |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208372 representing NM_001105731
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGCTGGAGGAAGACTTTGCCAAGATTCTCATGCTCAAGGAGGAGAGGATCAAAGAGCTGGAGA AGCGGCTGTCAGAGAAAGAGGAAGAAATCCAGGAGCTGAAGAGGAAACTCCACAAATGCCAGTCGGTGCT GCCCGTGCCCTCCACCCACATTGGCCCGCGGACCACCCGGGCGCAGGGCATCTCCGCGGAGCCGCAGACC TACAGGTCCTTCCACGACCTGCGCCAGGCGTTCCGGAAGTTCACTAAATCCGAGAGGTCGAAGGATCTCA TAAAGGAGGCTATCCTTGACAATGACTTCATGAAGAACTTGGAGCTGTCACAGATCCAGGAGATTGTGGA TTGTATGTACCCCGTGGAATATGGGAAGGACAGCTGCATCATCAAGGAAGGCGATGTGGGGTCACTGGTG TATGTCATGGAAGATGGGAAGGTCGAAGTCACAAAAGAAGGTGTGAAGTTGTGCACCATGGGTCCCGGGA AAGTGTTCGGGGAGCTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGT GAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATAATGATGAGAACAGGACTCATCAAGCACACC GAGTACATGGAGTTTTTAAAAAGTGTTCCAACATTCCAGAGCCTTCCTGATGAAATCCTCAGCAAGCTGG CTGATGTCCTCGAAGAGACTCACTATGAAAATGGAGAATATATCATAAGGCAGGGTGCAAGAGGAGACAC CTTCTTCATCATCAGCAAAGGGAAGGTAAGCGTTACCCGAGAAGACTCACCAAGTGAAGACCCAGTCTTT CTTAGAACTTTAGGGAAGGGAGATTGGTTTGAAGAGAAAGCCTTGCAGGGGGAGGATGTAAGAACAGCGA ACGTCATTGCTGCAGAGGCTGTCACCTGCCTCGTGATTGACAGAGACTCTTTCAAGCATTTAATTGGAGG ACTGGATGATGTCTCTAACAAAGCATATGAGGATGCAGAAGCGAAAGCAAAATATGAAGCCGAAGCTGCC TTCTTCGCCAACCTGAAGCTGTCTGACTTCAACATCATTGACACCCTTGGAGTGGGAGGTTTCGGACGAG TGGAGCTGGTCCAGTTGAAGAGTGAAGAATCTAAAACATTTGCGATGAAGATCCTCAAGAAACGACACAT TGTGGACACGAGACAGCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTC ATCGTGAGGCTATACCGGACATTTAAAGACAGCAAATACTTGTATATGTTGATGGAAGCTTGCCTAGGTG GAGAACTCTGGACTATTCTCAGGGATAGGGGTTCATTTGAAGACTCGACAACCAGATTTTACACAGCATG TGTGGTAGAAGCGTTTGCCTATCTGCATTCCAAAGGAATCATTTACAGGGACCTCAAGCCAGAAAATCTC ATCCTAGATCACCGAGGTTATGCCGAACTGGTTGACTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGA AAACATGGACTTTTTGTGGGACCCCAGAATACGTGGCCCCAGAGATCATCCTGAACAAAGGCCATGACAT TTCTGCTGACTACTGGTCACTAGGGATTCTGATGTATGAGCTTCTGACTGGCAGCCCACCTTTCTCAGGC CCAGATCCTATGAAAACCTACAACATCATACTGCGGGGGATTGACATGATAGAATTTCCAAAGAAGATTG CAAAAAGTGCTGCTAATTTAATTAAAAAACTATGCAGGGACAACCCATCAGAAAGGTTAGGAAATTTGAA AAACGGAGTCAAAGACATTCAAAAACACAAGTGGTTTGAGGGCTTTAACTGGGAAGGCTTAAGAAAAGGC ACCTTGACTCCTCCCATAATTCCGAGCGTTGCGTCGCCCACGGACACAAGCAATTTTGACAGTTTCCCTG AGGACAGCGACGAGCCACCGCCTGATGACAACTCAGGATGGGACATAGACTTCAGGACAGCGACGAGCCA CCGCCTGATGACAACTCAGGATGGGACATAGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208372 representing NM_001105731
Red=Cloning site Green=Tags(s) MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQT YRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLV YVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHT EYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVSVTREDSPSEDPVF LRTLGKGDWFEEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAA FFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDF IVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENL ILDHRGYAELVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSG PDPMKTYNIILRGIDMIEFPKKIAKSAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKG TLTPPIIPSVASPTDTSNFDSFPEDSDEPPPDDNSGWDIDFRTATSHRLMTTQDGT*T TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001105731 |
ORF Size | 2013 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2016 bp |
RefSeq ORF | 2016 bp |
Locus ID | 54286 |
Cytogenetics | 1q52 |
MW | 76.4 kDa |
Gene Summary | may play a role in cGMP mediated regulation of renal hemodynamics [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208372 | Prkg1 (untagged ORF) - Rat protein kinase, cGMP-dependent, type 1 (Prkg1), (10 ug) |
USD 677.00 |
|
RR208372L3 | Lenti ORF clone of Prkg1 (Myc-DDK-tagged ORF) - Rat protein kinase, cGMP-dependent, type 1 (Prkg1), (10 ug) |
USD 914.00 |
|
RR208372L4 | Lenti ORF clone of Prkg1 (mGFP-tagged ORF) - Rat protein kinase, cGMP-dependent, type 1 (Prkg1), (10 ug) |
USD 914.00 |
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