Prkg1 (NM_001105731) Rat Tagged ORF Clone

CAT#: RR208372

Prkg1 (Myc-DDK-tagged ORF) - Rat protein kinase, cGMP-dependent, type 1 (Prkg1), (10 ug)

Product Images

Circular map for RR208372

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkg1
• Synonyms: cGk1; Pkgi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208372 representing NM_001105731
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGCTGGAGGAAGACTTTGCCAAGATTCTCATGCTCAAGGAGGAGAGGATCAAAGAGCTGGAGA
  AGCGGCTGTCAGAGAAAGAGGAAGAAATCCAGGAGCTGAAGAGGAAACTCCACAAATGCCAGTCGGTGCT
  GCCCGTGCCCTCCACCCACATTGGCCCGCGGACCACCCGGGCGCAGGGCATCTCCGCGGAGCCGCAGACC
  TACAGGTCCTTCCACGACCTGCGCCAGGCGTTCCGGAAGTTCACTAAATCCGAGAGGTCGAAGGATCTCA
  TAAAGGAGGCTATCCTTGACAATGACTTCATGAAGAACTTGGAGCTGTCACAGATCCAGGAGATTGTGGA
  TTGTATGTACCCCGTGGAATATGGGAAGGACAGCTGCATCATCAAGGAAGGCGATGTGGGGTCACTGGTG
  TATGTCATGGAAGATGGGAAGGTCGAAGTCACAAAAGAAGGTGTGAAGTTGTGCACCATGGGTCCCGGGA
  AAGTGTTCGGGGAGCTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGT
  GAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATAATGATGAGAACAGGACTCATCAAGCACACC
  GAGTACATGGAGTTTTTAAAAAGTGTTCCAACATTCCAGAGCCTTCCTGATGAAATCCTCAGCAAGCTGG
  CTGATGTCCTCGAAGAGACTCACTATGAAAATGGAGAATATATCATAAGGCAGGGTGCAAGAGGAGACAC
  CTTCTTCATCATCAGCAAAGGGAAGGTAAGCGTTACCCGAGAAGACTCACCAAGTGAAGACCCAGTCTTT
  CTTAGAACTTTAGGGAAGGGAGATTGGTTTGAAGAGAAAGCCTTGCAGGGGGAGGATGTAAGAACAGCGA
  ACGTCATTGCTGCAGAGGCTGTCACCTGCCTCGTGATTGACAGAGACTCTTTCAAGCATTTAATTGGAGG
  ACTGGATGATGTCTCTAACAAAGCATATGAGGATGCAGAAGCGAAAGCAAAATATGAAGCCGAAGCTGCC
  TTCTTCGCCAACCTGAAGCTGTCTGACTTCAACATCATTGACACCCTTGGAGTGGGAGGTTTCGGACGAG
  TGGAGCTGGTCCAGTTGAAGAGTGAAGAATCTAAAACATTTGCGATGAAGATCCTCAAGAAACGACACAT
  TGTGGACACGAGACAGCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTC
  ATCGTGAGGCTATACCGGACATTTAAAGACAGCAAATACTTGTATATGTTGATGGAAGCTTGCCTAGGTG
  GAGAACTCTGGACTATTCTCAGGGATAGGGGTTCATTTGAAGACTCGACAACCAGATTTTACACAGCATG
  TGTGGTAGAAGCGTTTGCCTATCTGCATTCCAAAGGAATCATTTACAGGGACCTCAAGCCAGAAAATCTC
  ATCCTAGATCACCGAGGTTATGCCGAACTGGTTGACTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGA
  AAACATGGACTTTTTGTGGGACCCCAGAATACGTGGCCCCAGAGATCATCCTGAACAAAGGCCATGACAT
  TTCTGCTGACTACTGGTCACTAGGGATTCTGATGTATGAGCTTCTGACTGGCAGCCCACCTTTCTCAGGC
  CCAGATCCTATGAAAACCTACAACATCATACTGCGGGGGATTGACATGATAGAATTTCCAAAGAAGATTG
  CAAAAAGTGCTGCTAATTTAATTAAAAAACTATGCAGGGACAACCCATCAGAAAGGTTAGGAAATTTGAA
  AAACGGAGTCAAAGACATTCAAAAACACAAGTGGTTTGAGGGCTTTAACTGGGAAGGCTTAAGAAAAGGC
  ACCTTGACTCCTCCCATAATTCCGAGCGTTGCGTCGCCCACGGACACAAGCAATTTTGACAGTTTCCCTG
  AGGACAGCGACGAGCCACCGCCTGATGACAACTCAGGATGGGACATAGACTTCAGGACAGCGACGAGCCA
  CCGCCTGATGACAACTCAGGATGGGACATAGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208372 representing NM_001105731
  Red=Cloning site Green=Tags(s)
  
  MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQT
  YRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLV
  YVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHT
  EYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVSVTREDSPSEDPVF
  LRTLGKGDWFEEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAA
  FFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDF
  IVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENL
  ILDHRGYAELVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSG
  PDPMKTYNIILRGIDMIEFPKKIAKSAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKG
  TLTPPIIPSVASPTDTSNFDSFPEDSDEPPPDDNSGWDIDFRTATSHRLMTTQDGT*T
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105731
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2016 bp
• RefSeq ORF: 2016 bp
• Locus ID: 54286
• Cytogenetics: 1q52
• MW: 76.4 kDa
• Gene Summary: may play a role in cGMP mediated regulation of renal hemodynamics [RGD, Feb 2006]