Ugt8 (NM_019276) Rat Tagged ORF Clone

CAT#: RR208303

Ugt8 (Myc-DDK-tagged ORF) - Rat UDP glycosyltransferase 8 (Ugt8), (10 ug)

Product Images

Circular map for RR208303

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ugt8
• Synonyms: Ugt8a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208303 representing NM_019276
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCTTATACTCCATATTTCATGCTCCTGTGGAGTGCTGTTGGAATAGCAAGGGCTGCTAAAATCA
  TCATTGTGCCGCCAATTATGTTTGAAAGCCATTTGTACATTTTCAAGACACTAGCATCAGCCTTGCACGA
  GAGAGGCCATCACACTGTGTTCCTCCTCTCAGAAGGCAGAGACATTGACCCATCTAATCACTACAGCCTC
  CAGCGATACCCAGGGATCTTCAACAGTACCACCTCAGATGCTTTCCTGCAGTCTAAAATGCGGAATATTT
  TCTCTGGAAGATTGACAGCAGTTGAACTGGTTGACATACTGGATCACTATACTAAGAATTGTGACATGAT
  GGTTGGCAACCAAGCCCTAATCCAGGGTTTGAAAAAAGAAAAGTTTGACCTGCTACTGGTTGACCCCAAT
  GATATGTGTGGATTTGTGATCGCTCATCTTTTAGGCGTTAAGTATGCTGTGTTTTCTACTGGCCTTTGGT
  ATCCTGCTGAAGTTGGAGCGCCTGCTCCTTTAGCTTATGTCCCAGAGTTTAACTCACTCCTCACAGACCG
  CATGAACTTCCTGGAAAGGATGAAAAATACAGGCGTTTACCTCATATCCAGAATGGGGGTTAGCTTTCTG
  GTTCTTCCGAAATATGAAAGGATAATGCAGAAGTACAACCTGCTCCCTGCAAAGTCCATGTATGATTTGG
  TTCATGGGTCCAGCTTGTGGATGTTATGTACTGACGTAGCACTGGAGTTTCCAAGGCCCACCCTGCCTAA
  CGTTGTATATGTTGGAGGAATCCTAACAAAGCCGGCCAGCCCACTGCCAGAAGATCTGCAGAGGTGGGTA
  GATGGTGCTCAGGAGCATGGCTTTGTCCTGGTATCTTTTGGAGCTGGTGTCAAGTATCTGTCAGAAGACA
  TTGCTAACAAACTGGCAGGAGCTCTGGGGAGATTGCCTCAGAAAGTGATTTGGAGGTTTTCTGGAACCAA
  ACCAAAGAACCTAGGAAACAACACTAAGCTCATAGAATGGTTACCTCAAAATGACCTGCTTGGCCATTCA
  AACATCAGAGCCTTCCTAAGCCATGGTGGTTTGAACAGTATATTTGAAACTATGTATCATGGTGTCCCTG
  TAGTAGGAATCCCACTCTTTGGAGACCATTATGATACTATGACTAGAGTACAGGCGAAAGGCATGGGGAT
  CTTGTTAGAATGGAATACAGTTACTGAAGGGGAGCTGTATGATGCACTGGTGAAAGTTATCAACAATCCC
  AGTTATCGGCAGAGGGCTCAGAAGTTATCGGAAATTCATAAGGATCAACCCGGCCACCCTGTCAATCGGA
  CTACCTATTGGATAGACTACATTCTTCGCCACGACGGAGCCCATCACCTCCGTTCGGCTGTCCATCAGAT
  CTCCTTCTGTCAGTATTTTCTACTGGATATTGCCTTTGTGCTTCTGCTTGGTGCTGTCGCGCTCTACTTC
  ATAGTGTCCTATGTGACAAAATTTATCTATAGAAAAGTCAAAAGTCTGTGTTCTAGGAGTACGCACAGCA
  CAGTTAATGGACACTACCAAAATGGAATCCTCAATGGCAGATACAAAGGAAATGGTCATATTAAACATGA
  AAAAAAGGTAAAAAAAAAAGGTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208303 representing NM_019276
  Red=Cloning site Green=Tags(s)
  
  MKSYTPYFMLLWSAVGIARAAKIIIVPPIMFESHLYIFKTLASALHERGHHTVFLLSEGRDIDPSNHYSL
  QRYPGIFNSTTSDAFLQSKMRNIFSGRLTAVELVDILDHYTKNCDMMVGNQALIQGLKKEKFDLLLVDPN
  DMCGFVIAHLLGVKYAVFSTGLWYPAEVGAPAPLAYVPEFNSLLTDRMNFLERMKNTGVYLISRMGVSFL
  VLPKYERIMQKYNLLPAKSMYDLVHGSSLWMLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDLQRWV
  DGAQEHGFVLVSFGAGVKYLSEDIANKLAGALGRLPQKVIWRFSGTKPKNLGNNTKLIEWLPQNDLLGHS
  NIRAFLSHGGLNSIFETMYHGVPVVGIPLFGDHYDTMTRVQAKGMGILLEWNTVTEGELYDALVKVINNP
  SYRQRAQKLSEIHKDQPGHPVNRTTYWIDYILRHDGAHHLRSAVHQISFCQYFLLDIAFVLLLGAVALYF
  IVSYVTKFIYRKVKSLCSRSTHSTVNGHYQNGILNGRYKGNGHIKHEKKVKKKGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019276
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3481 bp
• RefSeq ORF: 1626 bp
• Locus ID: 50555
• UniProt ID: Q09426
• Cytogenetics: 2q42
• MW: 61.1 kDa
• Gene Summary: catalyzes the synthesis of galactocerebroside (Galc); important for the development and maintainance of the myelin membranes of the nervous system [RGD, Feb 2006]