Bloc1s2 (NM_001037349) Rat Tagged ORF Clone

CAT#: RR207557

Bloc1s2 (Myc-DDK-tagged ORF) - Rat biogenesis of lysosomal organelles complex-1, subunit 2 (Bloc1s2), (10 ug)

Product Images

Circular map for RR207557

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bloc1s2
• Synonyms: RSEP1; Sep1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207557 representing NM_001037349
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCGGAGGGCGTCCCGGCGACGCGACGGGAGGAGCCGCCTCGAGACGATGCTGCGGTGG
  AGACAGCTGAGGAAGCAAAGGAGCCTGCAGAAGCTGACATCAATGAACTCTGCCGGGACATGTTCTCCAA
  AATGGCCACATATTTGACTGGGGAACTGACAGCCACCAGTGAAGACTACAAACTCCTGGAAAATATGAAT
  AAGCTCACGAGCTTGAAGTACCTTGAAATGAAAGACATTGCTATAAACATCAGCAGAAACCTGAAGGACT
  TAAACCAGAAATATGCCGGACTGCAGCCATATCTGGATCAGATCAACGTGATTGAGGAGCAGGTGGCAGC
  TCTCGAGCAGGCAGCCTACAAGTTGGATGCTTATTCAAAGAAACTGGAAGCCAAGTACAAGAAGTTGGAG
  AAGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207557 representing NM_001037349
  Red=Cloning site Green=Tags(s)
  
  MAAAAEGVPATRREEPPRDDAAVETAEEAKEPAEADINELCRDMFSKMATYLTGELTATSEDYKLLENMN
  KLTSLKYLEMKDIAINISRNLKDLNQKYAGLQPYLDQINVIEEQVAALEQAAYKLDAYSKKLEAKYKKLE
  KR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037349
• ORF Size: 426 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037349.2, NP_001032426.1
• RefSeq Size: 936 bp
• RefSeq ORF: 429 bp
• Locus ID: 293938
• UniProt ID: Q32WR5
• Cytogenetics: 1q54
• MW: 16.1 kDa
• Gene Summary: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor. May play a role in cell proliferation.[UniProtKB/Swiss-Prot Function]