Kcnb1 (NM_013186) Rat Tagged ORF Clone
CAT#: RR207497
- TrueORF®
Kcnb1 (Myc-DDK-tagged ORF) - Rat potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_013186" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Kcnb1 |
Synonyms | DRK1PC; Kcr1-1; Kv2.1; Shab |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207497 representing NM_013186
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGAAGCATGGCTCGCGCTCCACCAGCTCGCTGCCGCCCGAGCCCATGGAGATCGTGCGCAGCAAGG CGTGCTCGCGCCGGGTGCGCCTCAACGTCGGGGGCCTGGCGCACGAGGTGCTGTGGCGCACTCTGGACCG CCTGCCCCGCACGCGGCTGGGCAAGCTTCGGGACTGCAACACGCACGACTCCCTGCTCCAGGTGTGCGAC GACTACAGCCTTGAGGACAACGAGTACTTCTTCGACCGCCACCCTGGCGCCTTTACCTCCATTCTCAATT TCTACCGCACCGGCCGGCTGCACATGATGGAGGAGATGTGCGCGCTGAGCTTCAGCCAGGAGCTGGACTA CTGGGGCATCGATGAGATCTACCTGGAGTCCTGCTGCCAGGCCCGCTATCACCAAAAGAAGGAGCAGATG AACGAGGAGCTGAAGCGCGAGGCCGAGACGCTGCGGGAGCGGGAGGGCGAGGAGTTTGACAACACGTGCT GTGCAGAGAAGAGGAAGAAACTCTGGGATCTGCTGGAGAAGCCCAACTCGTCGGTGGCCGCCAAGATCCT GGCCATCATCTCGATCATGTTCATCGTCCTGTCCACCATCGCTCTGTCACTCAACACGCTGCCTGAGCTG CAGAGCCTAGACGAGTTCGGCCAGAGCACGGATAACCCGCAGCTGGCGCACGTGGAGGCGGTGTGCATCG CATGGTTCACCATGGAGTACTTGCTGAGGTTCCTGTCCTCCCCCAAGAAATGGAAGTTCTTCAAGGGCCC CCTCAACGCCATTGACCTACTGGCCATCCTGCCCTACTACGTCACCATCTTCCTCACAGAATCCAACAAG AGCGTGCTGCAGTTCCAGAACGTGCGCCGCGTGGTCCAGATCTTTCGCATCATGCGCATCCTTCGGATCC TGAAGCTGGCCCGTCACTCCACTGGCCTGCAGTCCTTGGGCTTCACCCTGCGCAGAAGCTACAACGAACT GGGCCTGCTCATCCTCTTCCTCGCGATGGGCATCATGATCTTCTCCAGCCTGGTCTTCTTCGCGGAGAAG GATGAGGACGACACCAAGTTCAAAAGCATCCCCGCCTCTTTCTGGTGGGCTACCATCACCATGACAACTG TTGGCTATGGAGACATCTACCCGAAGACTCTCCTGGGGAAAATCGTGGGGGGGCTCTGTTGCATTGCCGG GGTCCTGGTGATTGCTCTCCCCATCCCCATCATCGTCAATAACTTCTCCGAGTTCTACAAGGAGCAGAAG CGCCAGGAGAAAGCCATCAAGCGGAGGGAGGCTCTGGAGAGAGCCAAGAGGAATGGCAGCATCGTGTCCA TGAACATGAAGGACGCCTTCGCCCGGAGCATCGAGATGATGGACATCGTGGTGGAGAAAAATGGCGAGAG CATAGCTAAGAAGGATAAAGTGCAAGATAACCACCTGTCCCCCAACAAGTGGAAATGGACCAAGAGGGCA CTCTCCGAGACCAGCTCCAGTAAGTCCTTTGAAACCAAGGAGCAGGGATCCCCCGAAAAGGCCAGGTCCT CGTCTAGTCCACAGCACTTGAACGTCCAGCAGCTGGAAGACATGTACAGTAAGATGGCCAAGACACAGTC CCAGCCCATCCTCAACACCAAGGAGATGGCCCCGCAGAGCAAGCCTCCAGAGGAGCTGGAGATGAGCAGC ATGCCCAGCCCCGTGGCCCCTCTGCCCGCACGCACGGAGGGCGTCATCGACATGCGGAGCATGTCCAGCA TTGACAGCTTCATCAGCTGTGCCACGGACTTCCCTGAAGCCACCAGATTCTCCCACAGTCCTCTGGCATC CCTCTCCAGCAAGGCTGGGAGCAGCACAGCCCCGGAGGTGGGCTGGCGGGGGGCTCTGGGTGCCAGCGGC GGGAGGCTCACGGAGACCAACCCCATTCCTGAGACCAGCCGCTCTGGTTTCTTCGTGGAGAGTCCCAGGA GTTCCATGAAGACCAACAACCCCTTGAAGCTGCGAGCGCTCAAGGTCAACTTCGTGGAGGGCGACCCCAC CCCGCTGCTGCCCTCCCTGGGCTTGTATCACGATCCTCTTAGGAACAGAGGAGGGGCAGCGGCTGCAGTT GCGGGACTGGAGTGTGCCTCCCTCTTAGACAAGCCCGTGCTGAGCCCAGAGTCCTCCATCTACACCACAG CAAGTGCCAGGACGCCCCCTCGCTCCCCTGAGAAACACACAGCAATAGCATTCAACTTCGAGGCGGGAGT CCACCATTACATAGACACCGACACAGATGACGAGGGTCAGCTGCTCTACAGCGTGGACTCCAGCCCTCCC AAAAGTCTCCACGGGAGTACCAGTCCCAAGTTCAGCACTGGAGCTAGAACGGAGAAGAACCACTTCGAGA GCTCCCCCTTGCCCACCTCCCCTAAGTTCTTAAGGCCGAACTGTGTCTACTCCTCAGAAGGGTTGACTGG AAAAGGCCCTGGGGCTCAAGAGAAATGCAAGCTGGAGAACCATACCCCCCCGGATGTGCACATGCTGCCT GGGGGAGGAGCACACGGGAGCACTAGGGATCAGAGTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207497 representing NM_013186
Red=Cloning site Green=Tags(s) MTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLQVCD DYSLEDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQM NEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPEL QSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNK SVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEK DEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQK RQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGESIAKKDKVQDNHLSPNKWKWTKRA LSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLEDMYSKMAKTQSQPILNTKEMAPQSKPPEELEMSS MPSPVAPLPARTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSSKAGSSTAPEVGWRGALGASG GRLTETNPIPETSRSGFFVESPRSSMKTNNPLKLRALKVNFVEGDPTPLLPSLGLYHDPLRNRGGAAAAV AGLECASLLDKPVLSPESSIYTTASARTPPRSPEKHTAIAFNFEAGVHHYIDTDTDDEGQLLYSVDSSPP KSLHGSTSPKFSTGARTEKNHFESSPLPTSPKFLRPNCVYSSEGLTGKGPGAQEKCKLENHTPPDVHMLP GGGAHGSTRDQSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013186 |
ORF Size | 2559 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013186.1, NP_037318.1 |
RefSeq Size | 3348 bp |
RefSeq ORF | 2562 bp |
Locus ID | 25736 |
UniProt ID | P15387 |
Cytogenetics | 3q42 |
MW | 95.3 kDa |
Gene Summary | voltage gated potassium channel; involved in the excitability in nerve and muscle [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207497 | Kcnb1 (untagged ORF) - Rat potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), (10 ug) |
USD 860.00 |
|
RR207497L3 | Lenti ORF clone of Kcnb1 (Myc-DDK-tagged ORF) - Rat potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), (10 ug) |
USD 1,159.00 |
|
RR207497L4 | Lenti ORF clone of Kcnb1 (mGFP-tagged ORF) - Rat potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), (10 ug) |
USD 1,159.00 |
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