Slc29a2 (NM_031738) Rat Tagged ORF Clone

CAT#: RR207295

Slc29a2 (Myc-DDK-tagged ORF) - Rat solute carrier family 29 (nucleoside transporters), member 2 (Slc29a2), (10 ug)

Product Images

Circular map for RR207295

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc29a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207295 representing NM_031738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCACGGAAACGCCCCTCGGGACAGCTACCACCTGGTCGGGATCAGCTTCTTCATTTTGGGTCTGG
  GCACCCTCCTCCCCTGGAACTTCTTCATTACCGCCATCCCGTACTTCCAGGGGCGGTTGGCAGGGACCAA
  CAGCAGCGCTGAGACCCCGAGCACCAACCACACCAGTCCCACAGACACCTTCAACTTCAACAACTGGGTG
  ACACTGCTGTCCCAGCTGCCTCTACTGCTCTTCACGCTCCTCAACTCCTTCTTGTATCAGTGCATCCCTG
  AGTCGGTGCGTATTCTGGGCAGCCTGCTGGCCATACTACTGCTCTTTGCCCTGACAGCAGCGCTGGTGAA
  GGTGGATTTGAGCCCCGGGCTGTTCTTTTCCATCACCATGGCATCCGTCTGGTTCATCAATTCCTTCTGT
  GCAGTGCTTCAAGGCAGCCTCTTTGGGCAGCTGGGTACCATGCCTTCTACCTACAGCACCCTCTTCCTCA
  GTGGCCAGGGCCTGGCTGGGATCTTCGCTGCCCTTGCTATGCTCACGTCCTTAGCCAGTGGCGTGGACCC
  CCAGACCTCTGCCCTTGGGTACTTCATCACACCCTGTGTGGGCATCCTCCTCTCCATCATATGTTACCTC
  AGTCTGCCCCATCTGAAGTTTGCCCGTTACTACCTGACCAAGAAGCCTCAGGCCCCAGTTCAGGAGCTGG
  AGACCAAAGCTGAGCTCCTCGGAGCTGATGAGAAGAATGGGATTCCTGTCAGCCCCCAGCAGGCAGGCCC
  AACTCTGGATCTTGACCCAGAGAAGGAGCTGGAGCTGGGGCTGGAGGAACCACAGAAGCCAGGAAAACCT
  TCGGTCTTTGTCGTCTTCCGGAAGATCTGGCTGACGGCGCTGTGCCTTGTGTTGGTCTTCACAGTCACCC
  TGTCGGTCTTTCCTGCTATCACAGCCATGGTGACCACCAGCTCCAATAGCCCCGGGAAGTGGAGTCAGTT
  CTTCAACCCTATCTGTTGCTTCCTGCTCTTCAACGTCATGGATTGGCTGGGCCGGAGCCTGACTTCCTAC
  TTCCTGTGGCCAGATGAGGACAGCCAGCTGCTGCCCCTGCTGGTGTGCCTGCGCTTCCTGTTCGTGCCCC
  TCTTCATGCTGTGTCATGTGCCCCAGCGTGCCCGGCTGCCCATCATCTTCTGGCAGGATGCCTACTTCAT
  CACATTCATGCTGCTCTTCGCCATTTCCAACGGCTACTTCGTGTCTCTCACCATGTGTCTGGCACCCAGG
  CAGGTGCTGCCGCATGAGAGGGAGGTGGCCGGAGCCCTCATGACCTTCTTCCTGGCCCTGGGACTCTCCT
  GCGGAGCCTCTCTCTCCTTCCTCTTTAAGGCTTTACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207295 representing NM_031738
  Red=Cloning site Green=Tags(s)
  
  MAHGNAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQGRLAGTNSSAETPSTNHTSPTDTFNFNNWV
  TLLSQLPLLLFTLLNSFLYQCIPESVRILGSLLAILLLFALTAALVKVDLSPGLFFSITMASVWFINSFC
  AVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLTSLASGVDPQTSALGYFITPCVGILLSIICYL
  SLPHLKFARYYLTKKPQAPVQELETKAELLGADEKNGIPVSPQQAGPTLDLDPEKELELGLEEPQKPGKP
  SVFVVFRKIWLTALCLVLVFTVTLSVFPAITAMVTTSSNSPGKWSQFFNPICCFLLFNVMDWLGRSLTSY
  FLWPDEDSQLLPLLVCLRFLFVPLFMLCHVPQRARLPIIFWQDAYFITFMLLFAISNGYFVSLTMCLAPR
  QVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031738
• ORF Size: 1368 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031738.1, NP_113926.1
• RefSeq Size: 1678 bp
• RefSeq ORF: 1371 bp
• Locus ID: 65194
• UniProt ID: O54699
• Cytogenetics: 1q43
• MW: 50.3 kDa
• Gene Summary: nucleoside transporter [RGD, Feb 2006]