Lmnb1 (NM_053905) Rat Tagged ORF Clone
CAT#: RR206117
- TrueORF®
Lmnb1 (Myc-DDK-tagged ORF) - Rat lamin B1 (Lmnb1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_053905" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Lmnb1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206117 representing NM_053905
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGCGACCCCCGTGCAGCAGCGGGCGGGCAGCCGCGCCAGCGCCCCCGCCACGCCGTTCAGCC CCACGCGCCTGTCGCGGCTGCAGGAGAAAGAGGAGCTGCGCGAGCTCAACGACCGTCTGGCGGTGTATAT TGACAAGGTGCGCAGCTTGGAGACGGAGAACAGCGCGCTGCAGCTGCAGGTGACCGAGCGCGAGGAGGTG CGCGGCCGCGAGCTCACCGGGCTCAAGGCTCTCTACGAGACCGAGCTGGCCGACGCACGCCGCGCTCTAG ACGACACGGCCCGCGAGCGCGCCAAGCTACAGATCGAGCTGGGCAAGTTCAAGGCCGAGCACGACCAGCT GCTGCTCAATTATGCTAAGAAGGAGTCTGATCTCAGTGGAGCCCAGATCAAGCTTCGAGAGTATGAGGCC GCGCTAAACTCTAAGGATGCAGCACTGGCTACTGCCCTTGGGGACAAAAAGAGTTTAGAGGGAGACTTGG AGGATCTGAAAGACCAGATTGCCCAGTTGGAAGCATCCCTATCTGCCGCCAAAAAGCAGTTGGCGGATGA GACTTTGCTTAAAGTGGATCTGGAGAACCGTTGTCAGAGCCTTACTGAGGACTTGGAGTTCCGTAAAAAT ATGTATGAAGAGGAGATCAATGAGACAAGAAGGAAGCATGAGACGCGCCTGGTGGAGGTGGACTCCGGGC GTCAGATTGAGTATGAGTACAAGCTGGCTCAAGCCCTGCACGAGATGAGAGAGCAGCACGACGCGCAGGT GAGGCTGTATAAGGAAGAGCTGGAGCAGACCTACCACGCCAAGCTTGAGAATGCCAGGCTGTCATCAGAG ATGAACACTTCCACTGTCAACAGTGCCAGGGGAGGAATGATGGAGAGCCGGATGAGGATCGAGAGCCTTT CCTCTCAGCTTTCTAACCTGCAGAAAGATTCTAGAGCATGCTTGGAAAGGATTCAAGAATTGGAGGACAT GCTTGCTAAAGAGAGAGACAACTCACGCCGCATGCTGTCTGACAAAGAGAGAGAGATGGCGGAAATAAGG GACCAGATGCAGCAGCAGCTGAATGATTACGAACAGCTGCTTGACGTGAAGCTGGCCCTGGACATGGAGA TCAGCGCCTACAGGAAACTCTTGGAAGGCGAAGAAGAGCGGTTAAAGCTCTCTCCAAGTCCTTCTTCCCG AGTGACCGTGTCCAGAGCCTCGTCCAGCCGCAGTGTGCGCACCACCAGAGGAAAGCGGAAGAGAGTTGAT GTGGAAGAATCAGAGGCGAGCAGTAGTGTTAGCATTTCCCACTCTGCTTCAGCCACGGGGAATGTGTGCA TTGAAGAGATAGATGTTGATGGGAAGTTTATTCGCTTGAAGAACACTTCTGAACAGGATCAACCAATGGG AGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTCAGTTACAAATATACCTCAAGATATGTGCTG AAGGCCGGCCAGACTGTTACAGTATGGGCTGCAAATGCTGGAGTCACAGCCAGTCCTCCAACTGACCTCA TCTGGAAGAACCAGAACTCTTGGGGCACTGGCGAGGATGTGAAGGTTGTGTTGAAGAATTCTCAGGGAGA GGAGGTTGCTCAGAGAAGCACAGTCTTCAAAACTACCATACCTGAGGAGGAGGAGGAGGAGGAGGAGGAG CCCATCGGAGTGCCCCTGGAGGAGGAACGTTTCCACCAGCAGGGAACCCCAAGAGCATCCAATAAAAGCT GTGCCATTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206117 representing NM_053905
Red=Cloning site Green=Tags(s) MATATPVQQRAGSRASAPATPFSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEV RGRELTGLKALYETELADARRALDDTARERAKLQIELGKFKAEHDQLLLNYAKKESDLSGAQIKLREYEA ALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLSAAKKQLADETLLKVDLENRCQSLTEDLEFRKN MYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSE MNTSTVNSARGGMMESRMRIESLSSQLSNLQKDSRACLERIQELEDMLAKERDNSRRMLSDKEREMAEIR DQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVD VEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVL KAGQTVTVWAANAGVTASPPTDLIWKNQNSWGTGEDVKVVLKNSQGEEVAQRSTVFKTTIPEEEEEEEEE PIGVPLEEERFHQQGTPRASNKSCAIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053905 |
ORF Size | 1761 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053905.1, NP_446357.1 |
RefSeq Size | 2154 bp |
RefSeq ORF | 1764 bp |
Locus ID | 116685 |
UniProt ID | P70615 |
Cytogenetics | 18q12.1 |
MW | 66.6 kDa |
Gene Summary | human homolog is a nuclear lamina protein; involved in nuclear stability, chromatin structure and gene expression [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206117 | Lmnb1 (untagged ORF) - Rat lamin B1 (Lmnb1), (10 ug) |
USD 602.00 |
|
RR206117L3 | Lenti ORF clone of Lmnb1 (Myc-DDK-tagged ORF) - Rat lamin B1 (Lmnb1), (10 ug) |
USD 901.00 |
|
RR206117L4 | Lenti ORF clone of Lmnb1 (mGFP-tagged ORF) - Rat lamin B1 (Lmnb1), (10 ug) |
USD 901.00 |
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