Egr2 (NM_053633) Rat Tagged ORF Clone

CAT#: RR206025

Egr2 (Myc-DDK-tagged ORF) - Rat early growth response 2 (Egr2), (10 ug)

Product Images

Circular map for RR206025

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egr2
• Synonyms: Krox20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206025 representing NM_053633
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTATGCACCAGCTGCCTGACA
  GCCTCTACCCGGTGGAAGACCTCGCCGCCCCGTCGGTGACCATCTTCCCCAATGGTGAACTGGGAGGCCC
  CTTTGATCAGATGAACGGAGTGGCTGGAGATGGCATGATCAACATTGACATGACCGGAGAGAAGAGGCCC
  TTGGATCTCCCATATCCGAGTAGCTTCGCTCCCATCTCTGCGCCTAGAAACCAGACCTTCACTTACATGG
  GCAAATTCTCCATTGACCCTCAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATCATCAATATTGTGAG
  TGCGGGCATCTTGCAAGGGGTCACCCCTCCAGCTTCAACCACAGCCTCTTCCAGCGTCACCTCTGCCTCC
  CCCAACCCACTGGCCACGGGACCCCTGGGTGTGTGTACCATGTCCCAGACTCAGCCTGAACTGGACCACC
  TCTACTCTCCACCACCACCTCCTCCTCCTTATTCGGGCTGTACAGGAGACCTCTACCAGGATCCTTCAGC
  ATTCTTATCGCCGCCACCCACCACTTCCACCTCCTCTCTGGCCTACCAGCCACCTCCTTCCTACCCATCC
  CCCAAGCCGGCTATGGACCCAGGTCTCATTCCTATGATCCCAGACTATCCTGGATTTTTTCCATCTCCGT
  GCCAGAGAGATCCACATGGCGCGGCTGGCCCTGATCGAAAGCCGTTCCCCTGCCCTCTGGACTCCCTGCG
  GGTCCCCCCTCCGCTCACGCCACTCTCCACCATCCGTAATTTTACTCTTGGGGGGCCCAGTGCTGGCGTC
  ACGGGACCAGGGGCAAGTGGAGGCGGTGAGGGTCCCCGACTGCCTGGCAGTGGGTCTGCAGCAGTGACTG
  CTACCCCCTACAATCCGCACCACCTGCCTTTAAGGCCCATCCTGAGACCTCGAAAGTACCCCAACAGGCC
  CAGCAAGACGCCAGTGCACGAAAGGCCGTACCCCTGCCCAGCAGAGGGTTGCGACAGGAGGTTCTCACGC
  TCTGATGAGCTGACCAGGCACATCCGAATCCACACCGGTCACAAGCCCTTCCAGTGTCGGATCTGCATGC
  GAAACTTCAGCCGAAGTGACCACCTCACCACTCACATCCGCACCCACACCGGGGAGAAGCCCTTTGCCTG
  TGACTATTGTGGCCGTAAGTTTGCCCGGAGTGACGAAAGGAAGCGCCACACCAAGATCCACCTTCGCCAG
  AAGGAGAGGAAGAGCAGTGCCCCCTCGTCATCTGCATCCGCCCAGTCTTCAGCCTCTGGTCCTGGGGGCT
  CGCAGGCCGGGGGCAGCCTGTGCGGTAACAGCGCCATTGGAGGACCACTGGCCTCCTGCACCTCTCGGAC
  CAGGACCCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206025 representing NM_053633
  Red=Cloning site Green=Tags(s)
  
  MMTAKAVDKIPVTLSGFMHQLPDSLYPVEDLAAPSVTIFPNGELGGPFDQMNGVAGDGMINIDMTGEKRP
  LDLPYPSSFAPISAPRNQTFTYMGKFSIDPQYPGASCYPEGIINIVSAGILQGVTPPASTTASSSVTSAS
  PNPLATGPLGVCTMSQTQPELDHLYSPPPPPPPYSGCTGDLYQDPSAFLSPPPTTSTSSLAYQPPPSYPS
  PKPAMDPGLIPMIPDYPGFFPSPCQRDPHGAAGPDRKPFPCPLDSLRVPPPLTPLSTIRNFTLGGPSAGV
  TGPGASGGGEGPRLPGSGSAAVTATPYNPHHLPLRPILRPRKYPNRPSKTPVHERPYPCPAEGCDRRFSR
  SDELTRHIRIHTGHKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDYCGRKFARSDERKRHTKIHLRQ
  KERKSSAPSSSASAQSSASGPGGSQAGGSLCGNSAIGGPLASCTSRTRTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053633
• ORF Size: 1410 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053633.1, NP_446085.1
• RefSeq Size: 2976 bp
• RefSeq ORF: 1413 bp
• Locus ID: 114090
• UniProt ID: P51774
• Cytogenetics: 20p11
• MW: 50.3 kDa
• Gene Summary: binds DNA; may play a role in learning and long term potentiation [RGD, Feb 2006]