Hexim1 (NM_001025136) Rat Tagged ORF Clone

CAT#: RR205491

Hexim1 (Myc-DDK-tagged ORF) - Rat hexamethylene bis-acetamide inducible 1 (Hexim1), (10 ug)

Product Images

Circular map for RR205491

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hexim1
• Synonyms: Clp-1; Clp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205491 representing NM_001025136
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGCCACTCTTGTCAGAACATCAACACCAGCCTCAAACTAGCAACTGTACAGGTGCTGCTGTTG
  TCCATGAGGAGCAGAACTCTGAGCGCCCCCCAAGCGCGGAGGAACGGGTGCCTAAGGAGGACAGTAGGTG
  GCAATCGAGAGCGTCCTTGCAGTCGGGTAGCCGTCCAGGGCAGGAGGGAGACGGGGGCCTGAAGCACCAG
  CTGCCCCCATTGCAGACGAATGCCTGTCCAGAATTGAGCTGCTTGGAAAAGGGCGAGAAGGGCCAGAATG
  GGGAGGACTTATCCACTGGCGGTGCCTCGCCTTCGGCGGAGGGGGAGCCGATGTCAGAGTCCCTCGTGCA
  ACCAGGTCATGACTCGGAGGCTACTAAGCTGGAGGCTCCTGTCGCTGGAGTAGAGGAGCCATGGGGACAG
  CAACAGAGACAGCTGGGCAAGAAAAAACACCGGAGACGCCCCTCCAAGAAGAAGCGACATTGGAAGCCCT
  ATTACAAGCTGACTTGGGAGGAGAAGAAAAAGTTCGACGAGAAGCAAAGCCTGCGAGCTTCGCGAGTTCG
  AGCCGAGATGTTCGCCAAGGGCCAGCCGGTTGCGCCCTATAACACCACGCAGTTCCTCATGGATGACCAT
  GATCAGGAGGAGCCTGATCTCAAAACCGGCCTTTACCCTAAGCGGGCAGCCGCCAAATCCGACGACACCA
  GCGATGAGGATTTCGTGGAAGAAGCTGGTGAGGAAGACGGAGGCAGCGATGGCATGGGAGGGGACGGCAG
  CGAGTTTCTGCAGCGGGACTTTTCCGAGACTTACGAGCGGTACCACGCCGAGAGCCTGCAGAACATGAGC
  AAGCAGGAGCTCATCAAAGAGTACCTGGAGCTGGAGAAGTGCCTCTCCCGCAAGGAGGACGAAAATAACC
  GGCTGCGGTTGGAAAGCAAGCGGTTGGGTGGCGTCGATGCGCGAGTGCGGGAGCTGGAGCTAGAGCTGGA
  CCGACTGCGCGCCGAGAACCGCCAGCTGCTGACCGAAAACGAACTGCACCGGCAGCAGGAGCGAGCGCCT
  CCTTCCAAGTTCGGCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205491 representing NM_001025136
  Red=Cloning site Green=Tags(s)
  
  MAEPLLSEHQHQPQTSNCTGAAVVHEEQNSERPPSAEERVPKEDSRWQSRASLQSGSRPGQEGDGGLKHQ
  LPPLQTNACPELSCLEKGEKGQNGEDLSTGGASPSAEGEPMSESLVQPGHDSEATKLEAPVAGVEEPWGQ
  QQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRVRAEMFAKGQPVAPYNTTQFLMDDH
  DQEEPDLKTGLYPKRAAAKSDDTSDEDFVEEAGEEDGGSDGMGGDGSEFLQRDFSETYERYHAESLQNMS
  KQELIKEYLELEKCLSRKEDENNRLRLESKRLGGVDARVRELELELDRLRAENRQLLTENELHRQQERAP
  PSKFGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025136
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025136.1, NP_001020307.1
• RefSeq Size: 2143 bp
• RefSeq ORF: 1071 bp
• Locus ID: 498008
• UniProt ID: Q5M9G1
• Cytogenetics: 10q32.1
• MW: 40.3 kDa
• Gene Summary: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.[UniProtKB/Swiss-Prot Function]