Sc5d (NM_053642) Rat Tagged ORF Clone
CAT#: RR205356
- TrueORF®
Sc5dl (Myc-DDK-tagged ORF) - Rat sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (Sc5dl), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_053642" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Sc5d |
Synonyms | Sc5dl |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205356 representing NM_053642
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGGTTCTCAGTGCTGCCGACTACTATTTCTTCACTCCCTATGTGTATCCAGCCACATGGCCCG AGGACAACATCATCCGACAAACTGTTAGTCTCCTGGTTGTCACCAACCTGGGTGCTTACATCCTCTACTT CTTCTGCGCGACCCTTAGCTATTATTTTGTCTATGACCATTCATTAATGAAACACCCACAGTTTTTAAAG AATCAAGTCTCTCGAGAGATCATGTTCACTGTCAAGTCTTTGCCTTGGATCAGCATCCCCACTGTGTCAT TGTTCCTGCTGGAGCTGAGGGGTTACAGCAAACTCTATGATGACATAGGAGACTTTCCAAATGGCTGGAT TCATCTCATCATGAGCGTGATCTCCTTCCTCTTCTTCACCGACATGTTGATCTACTGGATTCATAGGGGC CTGCACCACAGACTGCTCTATAAGCACATACATAAACCTCATCATATTTGGAAGATCCCCACGCCGTTCG CAAGTCATGCTTTTCACCCTGTGGACGGCTTCCTTCAGAGTCTGCCTTACCATATCTACCCCTTTGTCTT CCCGCTGCACAAGGTGGTCTACTTAGGTTTATATGTCTTGGTTAATGTCTGGACAATTTCTATTCATGAT GGCGACTTCCGGGTTCCCCAGATCTTCAGGCCGTTCATTAACGGGTCGGCTCATCACACAGACCACCACA TGCTCTTTGACTATAACTATGGGCAGTACTTCACACTGTGGGATAGAATCGGAGGCTCTTTTAAACATCC TTCCTCTTTCGAAGGGAAAGGACCACATAGTTACGTGAAGAACATGACAGAAAAAGAATCCAACAGCCTT GCAGAAAATGGCTGCAAAAGCAAAAAATTATGCAATGGGGAGTTTACAAAGAACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205356 representing NM_053642
Red=Cloning site Green=Tags(s) MDLVLSAADYYFFTPYVYPATWPEDNIIRQTVSLLVVTNLGAYILYFFCATLSYYFVYDHSLMKHPQFLK NQVSREIMFTVKSLPWISIPTVSLFLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRG LHHRLLYKHIHKPHHIWKIPTPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD GDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGKGPHSYVKNMTEKESNSL AENGCKSKKLCNGEFTKNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053642 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053642.2, NP_446094.1 |
RefSeq Size | 2185 bp |
RefSeq ORF | 900 bp |
Locus ID | 114100 |
UniProt ID | Q9EQS5 |
Cytogenetics | 8q22 |
MW | 35.1 kDa |
Gene Summary | human homolog is an enzyme involved in catalyzing the conversion of lathosterol into 7-dehydrocholesterol; involved in the synthesis of cholesterol [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205356 | Sc5dl (untagged ORF) - Rat sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (Sc5dl), (10 ug) |
USD 330.00 |
|
RR205356L3 | Lenti ORF clone of Sc5dl (Myc-DDK-tagged ORF) - Rat sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (Sc5dl), (10 ug) |
USD 630.00 |
|
RR205356L4 | Lenti ORF clone of Sc5dl (mGFP-tagged ORF) - Rat sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (Sc5dl), (10 ug) |
USD 630.00 |
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