Itga1 (NM_030994) Rat Tagged ORF Clone

CAT#: RR205013

Itga1 (Myc-DDK-tagged ORF) - Rat integrin alpha 1 (Itga1), (10 ug)

Product Images

Circular map for RR205013

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205013 representing NM_030994
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCCAGGCGTCCTGCCAGCCTAGAGGTCACTGTAGCCTGCATATGGCTTCTCACGGTCATCCTAG
  GCTTCTGCGTCTCCTTCAATGTTGATGTGAAAAACTCAATGAGTTTCAGTGGCCCAGTAGAGGACATGTT
  TGGATACACTGTTCAACAATATGAAAACGAAGAAGGCAAATGGGTTCTTATTGGTTCTCCTTTAGTTGGC
  CAACCCAAAGCAAGAACTGGAGATGTCTATAAGTGTCCGGTTGGGAGAGAGAGAGCAATGCCTTGCGTGA
  AGTTGGACTTGCCAGTTAACACATCGATCCCCAATGTCACAGAAATAAAGGAAAACATGACATTTGGATC
  AACTTTAGTCACCAACCCGAATGGAGGATTTCTGGCATGTGGGCCCTTGTATGCCTATAGATGTGGACAT
  TTGCATTATACAACTGGAATATGTTCTGATGTCAGTCCTACATTTCAAGTTGTGAACTCCTTTGCCCCTG
  TACAAGAATGCAGCACCCAGCTGGACATAGTCATCGTCCTGGATGGCTCCAACAGCATCTACCCCTGGGA
  AAGTGTCATCGCCTTTTTAAACGACCTTCTTAAGAGGATGGATATTGGCCCTAAGCAGACACAGGTCGGG
  ATTGTACAGTATGGAGAGAATGTAACCCATGAGTTCAACCTCAATAAGTATTCATCCACAGAAGAGGTCC
  TTGTCGCAGCAAACAAAATAGGCCGACAGGGAGGCCTCCAAACGATGACAGCCCTTGGAATAGACACAGC
  CAGGAAAGAGGCATTCACTGAAGCTCGGGGTGCCAGGAGGGGAGTTAAAAAAGTCATGGTTATTGTGACC
  GACGGAGAATCGCATGACAACTATCGCCTGAAACAGGTCATCCAAGACTGCGAGGACGAAAACATTCAGC
  GATTTTCCATAGCTATCCTTGGCCACTATAACAGGGGGAACTTAAGCACTGAAAAATTTGTGGAGGAAAT
  AAAATCGATCGCAAGCGAGCCCACGGAAAAGCACTTCTTCAATGTCTCGGATGAGTTGGCCCTGGTCACT
  ATTGTTAAAGCTCTGGGAGAAAGGATATTCGCTTTGGAAGCGACAGCTGACCAGTCAGCAGCTTCATTTG
  AGATGGAAATGTCTCAGACTGGCTTCAGTGCTCACTACTCCCAGGACTGGGTCATGCTTGGAGCGGTGGG
  AGCCTATGACTGGAACGGAACTGTGGTCATGCAGAAGGCTAACCAGATGGTCATCCCTCATAACACCACC
  TTTCAAACTGAGCCCGCCAAGATGAACGAGCCTCTGGCTTCTTATTTAGGTTACACAGTGAACTCGGCCA
  CCATCCCTGGAGATGTGCTCTACATCGCTGGGCAGCCTCGGTACAATCATACGGGCCAGGTCGTCATCTA
  CAAGATGGAGGATGGGAACATCAACATTCTGCAGACACTCGGCGGAGAGCAGATTGGTTCCTACTTTGGT
  AGTGTCTTAACAACAATTGACATCGACAAAGATTCTTATACTGATCTGCTTCTCGTCGGGGCCCCCATGT
  ACATGGGGACAGAGAAAGAGGAACAGGGCAAGGTGTACGTGTACGCTGTGAATCAGACAAGGTTTGAATA
  TCAAATGAGCCTGGAACCAATTAGGCAGACCTGCTGCTCATCCCTGAAGGATAATTCATGCACGAAAGAA
  AACAAGAATGAGCCCTGCGGGGCCCGCTTCGGAACAGCAATTGCTGCTGTAAAAGACCTCAACGTGGATG
  GATTTAATGACGTCGTGATTGGAGCTCCGCTGGAAGATGACCACGCAGGAGCTGTGTACATTTATCATGG
  CAGTGGCAAGACCATAAGGGAGGCGTATGCACAACGCATTCCATCAGGTGGGGATGGCAAGACCCTGAAA
  TTTTTCGGCCAGTCTATCCACGGAGAGATGGATTTAAATGGTGACGGTCTGACTGACGTGACCATTGGAG
  GCCTTGGTGGAGCAGCCCTCTTCTGGGCCAGAGATGTGGCTGTAGTTAAAGTGACCATGAATTTTGAACC
  CAATAAAGTGAATATTCAAAAGAAAAACTGCCGTGTGGAGGGCAAAGAAACAGTGTGCATAAATGCTACA
  ATGTGTTTTCATGTGAAATTAAAGTCTAAAGAGGACTCAATTTACGAGGCTGATCTGCAGTACCGTGTCA
  CCCTTGATTCACTGAGGCAGATATCACGGAGCTTTTTTTCTGGAACTCAGGAAAGGAAGATTCAAAGAAA
  TATCACCGTTCGAGAATCAGAATGCATCAGGCACTCCTTCTACATGTTGGACAAACATGACTTTCAGGAC
  TCTGTGAGAGTGACTCTGGATTTTAATCTCACTGATCCAGAAAATGGTCCTGTACTTGATGACGCTCTGC
  CAAACTCAGTCCACGAACACATTCCCTTTGCCAAAGACTGTGGAAACAAGGAAAGATGCATTTCAGACCT
  CACTCTGAATGTGTCCACCACAGAAAAGAGCCTGCTGATCGTCAAGTCCCAGCATGACAAGTTCAACGTT
  AGCCTCACCGTCAAAAACAAAGGAGACAGTGCGTACAACACCAGGACAGTGGTGCAGCATTCACCAAATC
  TGATTTTTTCGGGAATTGAGGAGATCCAAAAAGATAGCTGTGAATCTAATCAAAATATCACTTGCAGAGT
  TGGATATCCTTTCCTAAGAGCAGGAGAAACGGTTACCTTCAAAATAATATTCCAGTTTAACACATCCCAT
  CTCTCGGAAAATGCAATCATTCACTTAAGTGCAACAAGTGACAGTGAGGAGCCCCTGGAATCTCTTAATG
  ATAATGAAGTAAATATTTCCATCCCAGTAAAATATGAAGTTGGACTGCAGTTTTACAGTTCTGCGAGTGA
  ACATCACATTTCAGTCGCTGCCAATGAGACGATCCCTGAGTTTATTAACTCCACTGAGGACATTGGGAAT
  GAAATTAATGTCTTCTATACGATTAGAAAGAGGGGGCATTTCCCAATGCCAGAACTTCAGCTGTCAATTT
  CATTCCCCAATTTGACGGCAGATGGTTATCCTGTACTGTACCCAATTGGATGGTCATCTTCAGATAATGT
  GAACTGTAGACCCCGGAGCCTTGAGGACCCCTTTGGCATCAACTCTGGGAAGAAAATGACAATATCGAAG
  TCTGAGGTTCTCAAAAGAGGCACAATCCAGGACTGCAGTAGTACGTGTGGAGTTGCCACCATCACGTGTA
  GCCTCCTTCCTTCCGACCTGAGTCAAGTGAATGTCTCGCTCCTCCTGTGGAAACCGACTTTCATAAGAGC
  ACATTTTTCCAGCTTAAACCTTACTCTAAGAGGAGAACTTAAGAGTGAAAATTCATCGCTGACTTTAAGT
  AGCAGCAACCGGAAGCGAGAGCTGGCTATTCAGATATCCAAAGACGGGCTCCCAGGCAGAGTGCCGCTGT
  GGGTTATCCTCCTGAGCGCCTTCGCGGGGCTACTGCTGCTAATGCTCCTTATATTGGCTCTGTGGAAGAT
  TGGATTCTTCAAAAGGCCACTGAAGAAGAAAATGGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205013 representing NM_030994
  Red=Cloning site Green=Tags(s)
  
  MVPRRPASLEVTVACIWLLTVILGFCVSFNVDVKNSMSFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVG
  QPKARTGDVYKCPVGRERAMPCVKLDLPVNTSIPNVTEIKENMTFGSTLVTNPNGGFLACGPLYAYRCGH
  LHYTTGICSDVSPTFQVVNSFAPVQECSTQLDIVIVLDGSNSIYPWESVIAFLNDLLKRMDIGPKQTQVG
  IVQYGENVTHEFNLNKYSSTEEVLVAANKIGRQGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVT
  DGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVT
  IVKALGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKANQMVIPHNTT
  FQTEPAKMNEPLASYLGYTVNSATIPGDVLYIAGQPRYNHTGQVVIYKMEDGNINILQTLGGEQIGSYFG
  SVLTTIDIDKDSYTDLLLVGAPMYMGTEKEEQGKVYVYAVNQTRFEYQMSLEPIRQTCCSSLKDNSCTKE
  NKNEPCGARFGTAIAAVKDLNVDGFNDVVIGAPLEDDHAGAVYIYHGSGKTIREAYAQRIPSGGDGKTLK
  FFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWARDVAVVKVTMNFEPNKVNIQKKNCRVEGKETVCINAT
  MCFHVKLKSKEDSIYEADLQYRVTLDSLRQISRSFFSGTQERKIQRNITVRESECIRHSFYMLDKHDFQD
  SVRVTLDFNLTDPENGPVLDDALPNSVHEHIPFAKDCGNKERCISDLTLNVSTTEKSLLIVKSQHDKFNV
  SLTVKNKGDSAYNTRTVVQHSPNLIFSGIEEIQKDSCESNQNITCRVGYPFLRAGETVTFKIIFQFNTSH
  LSENAIIHLSATSDSEEPLESLNDNEVNISIPVKYEVGLQFYSSASEHHISVAANETIPEFINSTEDIGN
  EINVFYTIRKRGHFPMPELQLSISFPNLTADGYPVLYPIGWSSSDNVNCRPRSLEDPFGINSGKKMTISK
  SEVLKRGTIQDCSSTCGVATITCSLLPSDLSQVNVSLLLWKPTFIRAHFSSLNLTLRGELKSENSSLTLS
  SSNRKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030994
• ORF Size: 3540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030994.2, NP_112256.1
• RefSeq Size: 3963 bp
• RefSeq ORF: 3543 bp
• Locus ID: 25118
• UniProt ID: P18614
• Cytogenetics: 2q14
• MW: 130.8 kDa
• Gene Summary: alpha subunit of the integrin molecule; involved in binding laminin and collagen [RGD, Feb 2006]