Kcnh7 (NM_131912) Rat Tagged ORF Clone

CAT#: RR204616

Kcnh7 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, subfamily H (eag-related), member 7 (Kcnh7), (10 ug)

Product Images

Circular map for RR204616

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnh7
• Synonyms: erg3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204616 representing NM_131912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTTCGCAGGGGGCATGTGGCACCGCAAAACACCTTCCTGGGGACCATCATACGGAAATTTGAAG
  GGCAGAATAAAAAATTTATCATTGCAAATGCCAGAGTGCAGAACTGTGCCATCATCTACTGCAATGATGG
  CTTCTGTGAGATGACAGGTTTCTCCAGGCCAGATGTCATGCAGAAGCCATGCACCTGCGACTTTCTCCAT
  GGGCCTGAGACCAAGAGGCATGATATTGCCCAGATTGCCCAGGCGCTGTTGGGGTCAGAGGAGAGGAAAG
  TGGAGGTCACCTACTATCACAAGAATGGTTCCACTTTTATTTGTAACACTCACATAATCCCAGTAAAGAA
  CCAAGAGGGTGTGGCTATGATGTTCATCATTAACTTTGAGTATGTGACAGATGAAGACAATGCTGCCTCC
  CCAGAGAGAGTCAACCCAATATTGCCAGTCAAATCTGTAAATCGGAAACTTTTTGGGTTCAAATTTCCTG
  GTCTGAGAGTTCTAACATACAGAAAGCAGTCCTTGCCGCAGGAAGACCCGGACGTGGTAGTCATTGATTC
  TTCTAAGCACAGCGATGACTCTGTGGCTATGAAGCACTTTAAGTCTCCCACGAAAGAAAGCTGCAGTCCC
  TCTGAAGCAGATGATACGAAGGCCTTGATACAGCCTAGCCAGTGTTCTCCCTTAGTGAACATATCAGGAC
  CTCTGGACCATTCCTCTCCCAAAAGGCAATGGGACCGCCTCTACCCTGACATGCTGCAGTCAAGTTCCCA
  ACTAACACACTCCAGGTCAAGGGAGAGCCTCTGTAGCATACGGAGAGCATCTTCAGTTCATGATATAGAA
  GGGTTCAATGTCCACCCCAAGAACATATTTAGAGATCGACATGCCAGTGAAGACAATGGTCGAAATGTCA
  AAGGACCTTTCAATCATATCAAGTCAAGCCTGTTGGGATCCACATCAGATTCAAACCTCAACAAATACAG
  CACCATTAACAAGATCCCGCAACTCACTCTGAACTTTTCAGATGTCAAAACTGAGAAAAAGAATACTTCC
  CCGCCTTCTTCAGACAAAACTATTATTGCACCCAAGGTTAAAGAGAGGACACACAACGTGACAGAGAAGG
  TAACTCAGGTTCTTTCTTTGGGAGCAGATGTCTTGCCAGAATACAAGCTGCAGACGCCACGCATCAACAA
  ATTTACAATATTGCACTACAGCCCTTTCAAAGCAGTGTGGGATTGGCTCATTTTACTCCTGGTCATTTAT
  ACTGCTATATTCACCCCCTACTCGGCAGCCTTTCTCCTCAACGACAGAGAGGAGCAGAAAAGACGAGAAT
  GTGGCTATTCTTGTAGCCCTTTGAATGTGGTAGACTTGATTGTGGATATTATGTTTATTATAGACATCCT
  AATAAACTTCAGAACAACCTATGTAAATCAGAACGAAGAAGTGGTAAGTGATCCTGCCAAAATAGCAGTA
  CACTACTTCAAAGGCTGGTTCCTGATTGACATGGTCGCAGCCATCCCTTTTGACTTGCTGATTTTCGGAT
  CAGGCTCTGATGAGACAACAACACTAATTGGTCTTTTGAAGACTGCACGTCTCCTGCGTCTTGTGCGTGT
  AGCCAGGAAACTGGACCGATACTCAGAATATGGTGCCGCTGTTCTAATGCTCTTGATGTGCATATTTGCC
  CTGATTGCCCACTGGCTGGCTTGCATCTGGTATGCGATTGGGAATGTAGAGAGGCCCTATCTGACTGACA
  AAATTGGATGGTTGGATTCCTTAGGAACACAAATTGGGAAACGTTACAATGACAGTGACTCGAGTTCTGG
  ACCGTCCATTAAAGACAAATACGTCACGGCACTTTACTTTACCTTCAGCAGTCTAACCAGTGTAGGATTT
  GGAAATGTGTCTCCTAACACCAATTCGGAGAAAATCTTTTCCATTTGTGTCATGTTGATTGGCTCTCTAA
  TGTATGCGAGCATTTTTGGGAATGTTTCTGCAATTATTCAAAGACTGTACTCGGGAACTGCCAGGTACCA
  CATGCAGATGCTGAGAGTGAAAGAGTTCATTCGCTTCCACCAAATCCCCAACCCTCTGAGGCAACGGCTT
  GAGGAGTATTTCCAGCACGCATGGACGTACACTAACGGCATTGACATGAACATGGTCTTAAAGGGATTTC
  CGGAATGTTTACAAGCTGACATTTGCCTGCATCTAAACCAGACTTTGCTCCAAAACTGCAAAGCCTTTCG
  AGGAGCAAGTAAAGGTTGCCTCAGAGCTCTGGCAATGAAGTTCAAAACCACCCATGCCCCTCCAGGAGAC
  ACCCTGGTTCACTGTGGGGACGTCCTAACTGCACTGTACTTCTTATCCAGAGGCTCCATTGAAATCCTCA
  AGGATGACATAGTGGTAGCTATTCTAGGAAAAAATGATATCTTTGGAGAAATGGTTCATCTTTACGCCAA
  GCCTGGCAAATCTAATGCAGATGTGAGAGCGCTCACGTATTGTGACCTGCATAAGATTCAGCGAGAAGAC
  TTATTAGAGGTCTTGGATATGTATCCGGAATTTTCTGATCACTTTCTGACAAATCTAGAACTGACTTTCA
  ACCTGAGACACGAAAGTGCAAAGTCCCAATCCATAAATGATTCTGAAGGGGACACCTGTAAGCTTCGAAG
  AAGGAGATTGTCCTTTGAAAGCGAAGGCGACAAAGATTTTAGCAAAGAAAACAGTGCAAATGACGCTGAC
  GATTCCACAGATACAATAAGACGTTATCAGAGTTCCAAGAAGCACTTCGAAGAGAAGAAAAGCAGATCGT
  CTTCCTTTATCTCCTCCATCGATGATGAGCAAAAGCCACTCTTCCTGGGAACAGTAGATTCCACTCCAAG
  AATGGTGAAAGCAAGCAGGCACCATGGTGAAGAAGCAGCGCCCCCCTCAGGAAGAATTCACACAGATAAA
  AGGAGTCACTCCTGCAAAGATATCACTGACACACACAGCTGGGAAAGAGAGCATGCCCGGGCTCAGCCTG
  AAGAATGCAGTCCCTCCGGACTTCAGAGAGCTGCCTGGGGCATCTCTGAGACCGAAAGCGACCTCACCTA
  TGGGGAAGTGGAGCAGAGGTTAGATTTGCTTCAAGAGCAACTTAACAGACTTGAATCCCAAATGACAACG
  GACATCCAGGCCATCTTACAGCTCCTGCAGAAACAAACCACCGTAGTCCCTCCAGCCTACAGCATGGTGA
  CCGCAGGAGCAGAGTACCAGAGGCCCATCCTCCGTCTGCTGAGAACCAGTCACCCTAGAGCATCCATTAA
  GACAGACCGGAGCTTCAGCCCCTCCTCACAATGTCCTGAATTTCTCGACCTTGAAAAGTCCAAACTCAAA
  TCCAAAGAATCACTGTCAAGCGGAAAGCGCCTGAACACGGCTTCAGAAGACAACTTGACTTCACTTTTAA
  AACAAGACAGTGATGCATCGTCAGAGCTTGACCCGCGGCAAAGAAAATCTTACCTTCATCCCATCCGGCA
  TCCGTCTCTGCCAGACTCCTCCCTAAGCACTGTAGGGATCTTGGGTCTCCATAGGCATGTTTCTGATCCT
  GGTCTTCCGGGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204616 representing NM_131912
  Red=Cloning site Green=Tags(s)
  
  MPVRRGHVAPQNTFLGTIIRKFEGQNKKFIIANARVQNCAIIYCNDGFCEMTGFSRPDVMQKPCTCDFLH
  GPETKRHDIAQIAQALLGSEERKVEVTYYHKNGSTFICNTHIIPVKNQEGVAMMFIINFEYVTDEDNAAS
  PERVNPILPVKSVNRKLFGFKFPGLRVLTYRKQSLPQEDPDVVVIDSSKHSDDSVAMKHFKSPTKESCSP
  SEADDTKALIQPSQCSPLVNISGPLDHSSPKRQWDRLYPDMLQSSSQLTHSRSRESLCSIRRASSVHDIE
  GFNVHPKNIFRDRHASEDNGRNVKGPFNHIKSSLLGSTSDSNLNKYSTINKIPQLTLNFSDVKTEKKNTS
  PPSSDKTIIAPKVKERTHNVTEKVTQVLSLGADVLPEYKLQTPRINKFTILHYSPFKAVWDWLILLLVIY
  TAIFTPYSAAFLLNDREEQKRRECGYSCSPLNVVDLIVDIMFIIDILINFRTTYVNQNEEVVSDPAKIAV
  HYFKGWFLIDMVAAIPFDLLIFGSGSDETTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLMLLMCIFA
  LIAHWLACIWYAIGNVERPYLTDKIGWLDSLGTQIGKRYNDSDSSSGPSIKDKYVTALYFTFSSLTSVGF
  GNVSPNTNSEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHMQMLRVKEFIRFHQIPNPLRQRL
  EEYFQHAWTYTNGIDMNMVLKGFPECLQADICLHLNQTLLQNCKAFRGASKGCLRALAMKFKTTHAPPGD
  TLVHCGDVLTALYFLSRGSIEILKDDIVVAILGKNDIFGEMVHLYAKPGKSNADVRALTYCDLHKIQRED
  LLEVLDMYPEFSDHFLTNLELTFNLRHESAKSQSINDSEGDTCKLRRRRLSFESEGDKDFSKENSANDAD
  DSTDTIRRYQSSKKHFEEKKSRSSSFISSIDDEQKPLFLGTVDSTPRMVKASRHHGEEAAPPSGRIHTDK
  RSHSCKDITDTHSWEREHARAQPEECSPSGLQRAAWGISETESDLTYGEVEQRLDLLQEQLNRLESQMTT
  DIQAILQLLQKQTTVVPPAYSMVTAGAEYQRPILRLLRTSHPRASIKTDRSFSPSSQCPEFLDLEKSKLK
  SKESLSSGKRLNTASEDNLTSLLKQDSDASSELDPRQRKSYLHPIRHPSLPDSSLSTVGILGLHRHVSDP
  GLPGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_131912
• ORF Size: 3585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_131912.1, NP_571987.1
• RefSeq Size: 3807 bp
• RefSeq ORF: 3588 bp
• Locus ID: 170739
• UniProt ID: O54852
• Cytogenetics: 3q21
• MW: 134.9 kDa
• Gene Summary: produces a current that has a large transient component at positive potentials [RGD, Feb 2006]