Mccc1 (NM_001009653) Rat Tagged ORF Clone

CAT#: RR204283

Mccc1 (Myc-DDK-tagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) (Mccc1), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR204283

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mccc1
• Synonyms: MGC95141
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204283 representing NM_001009653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCTCTGTTGGCTGCGGTGGACAGGAACCAGTTGCGGCGGGTCCCGATCCTGCTGCTGC
  AGCCGAGGGAATGGCCTTGGAAGCACAGAACCGTGAAATATGGAACAACCCCAGGAGGAAGCATCACCAA
  GGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGGTGATACGAACAGCCAGAAAAATGGGTGTACAA
  TCTGTGGCTGTTTACAGCGAGGCCGACAGGAATTCCATGCATGTAGATATGGCAGACGAGGCGTATTCCA
  TTGGCCCTGCGCCATCTCAGCAGAGCTACCTCGCAATGGAGAAAATCATCCAAGTGGCCAAGAGCTCTGC
  AGCACAGGCCATCCATCCAGGATATGGTTTTCTTTCAGAAAATATGGAGTTTGCTGAATTTTGTAAGCAA
  GAAGGAATTATTTTTATAGGTCCTCCTTCAACTGCAATTAGAGACATGGGTATAAAGAGCACATCCAAGT
  CCATCATGGCTGCCGCTGGAGTACCTGTTGTGGAAGGTTACCATGGCAACGACCAATCTGATGAGTGCCT
  GAAAGAGCATGCAGGGAAAATCGGGTATCCTGTGATGATCAAAGCCATCCGTGGTGGAGGAGGAAAAGGC
  ATGAGGATCATTAGGTCAGAAAAAGAATTCCAAGAGCAGCTAGAATCAGCCCGGAGGGAGGCAAAGAAGT
  CTTTCAACGATGACGCAATGCTGATCGAGAAGTTCGTGGACACGCCAAGGCATGTGGAAGTCCAGGTGTT
  TGGTGATCACCATGGCAATGCAGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGGCGACATCAGAAG
  ATCATTGAGGAGGCCCCAGCGCCTGGTATTGATCCTGAAGTAAGAAGGAGGCTGGGAGAGGCTGCAGTCA
  GAGCTGCGAAGGCTGTCAACTATGTGGGAGCAGGAACTGTGGAATTCATTATGGACTCAAAGCACAATTT
  TTATTTCATGGAGATGAACACAAGGCTACAAGTGGAGCATCCGGTCACTGAGATGATCACGGGGACTGAC
  TTGGTGGAGTGGCAGCTCAGGATTGCAGCAGGGGAAAAGATCCCTTTGAGCCAGGAAGAAATCCCTCTGC
  AGGGCCATGCCTTTGAGGCCAGAATCTATGCAGAGGATCCTGACAATAACTTCATGCCAGGAGCTGGACC
  ATTAGTTCACCTCTCTACCCCTCCGCCAGACATGTCTACAAGGATCGAAACTGGAGTACGGCAAGGAGAT
  GAAGTCTCGGTGCATTACGACCCCATGATCGCAAAGCTGGTGGTGTGGGCCTCAGATCGCCAGTCAGCCC
  TGTCAAAACTGAGATACAGTCTTCATCAGTACAACATTGTTGGCTTGCGCACCAATGTGGACTTCCTGCT
  CCGGCTCTCCGGCCACTCAGAGTTTGAAGCTGGGAATGTACACACTGACTTCATCCCTCAACACCACAAG
  GACCTGCTGCCCACTCACAGCACCATAGCCAAGGAATCTGTGTGCCAGGCAGCCTTGGGGCTTATCCTCA
  AAGAGAAAGAAATGACCAGCGCTTTTAAACTCCACACTCAAGATCAATTCTCTCCGTTTTCATTCAGCAG
  TGGGAGAAGACTGAATATCTCTTACACCAGGAACATGACTTTAAGAAGTGGTAAAAACGATATAATCATA
  GCTGTGACGTATAACCGAGATGGGTCATATGACATGCAGATTGAAAATAAGTTATTCCGAGTCCTGGGCG
  ATCTTTCCAATGAGGATGGCTATACCTACCTGAAGTCTTCTGTTAATGGAGTAGCCAGTAAATCCAAGTT
  CATCCTCCTGGACAACACCATCTACCTGTTTTCTATGGAAGGCAGTATTGAAGTTGGCATTCCAGTACCC
  AAGTACTTGTCTCCAGTGAGTGCAGAAGGAACTCAGGGAGGCACCATCGCTCCCATGACTGGAACCATTG
  AAAAGGTGTTCGTGAAGGCTGGAGACAGAGTAAAGGCTGGAGACGCTCTGATGGTTATGATCGCCATGAA
  GATGGAGCACACCATAAAAGCCCCAAAGGACGGCAGGATAAAAAAGGTGTTTTTCAGTGAAGGAGCCCAA
  GCCAACAGACATGCACCTTTAGTGGAGTTTGAAGAGGAGGAAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204283 representing NM_001009653
  Red=Cloning site Green=Tags(s)
  
  MAAAALLAAVDRNQLRRVPILLLQPREWPWKHRTVKYGTTPGGSITKVLIANRGEIACRVIRTARKMGVQ
  SVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAEFCKQ
  EGIIFIGPPSTAIRDMGIKSTSKSIMAAAGVPVVEGYHGNDQSDECLKEHAGKIGYPVMIKAIRGGGGKG
  MRIIRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQK
  IIEEAPAPGIDPEVRRRLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTD
  LVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPPPDMSTRIETGVRQGD
  EVSVHYDPMIAKLVVWASDRQSALSKLRYSLHQYNIVGLRTNVDFLLRLSGHSEFEAGNVHTDFIPQHHK
  DLLPTHSTIAKESVCQAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKNDIII
  AVTYNRDGSYDMQIENKLFRVLGDLSNEDGYTYLKSSVNGVASKSKFILLDNTIYLFSMEGSIEVGIPVP
  KYLSPVSAEGTQGGTIAPMTGTIEKVFVKAGDRVKAGDALMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQ
  ANRHAPLVEFEEEEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009653
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009653.1, NP_001009653.1
• RefSeq Size: 2409 bp
• RefSeq ORF: 2148 bp
• Locus ID: 294972
• UniProt ID: Q5I0C3
• Cytogenetics: 2q25
• MW: 79.3 kDa
• Gene Summary: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.[UniProtKB/Swiss-Prot Function]