Atf6 (NM_001107196) Rat Tagged ORF Clone

CAT#: RR204267

Atf6 (Myc-DDK-tagged ORF) - Rat activating transcription factor 6 (Atf6), (10 ug)

Product Images

Circular map for RR204267

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atf6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204267 representing NM_001107196
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGCCTTTTAGTCCGGTTCTTCCTCATGGACCAGGTGAAGACTGGGAGTCCACGTTGTTTGCTG
  AGCTTGGCTATTTCACAGACACGGATGATGTCCAGTTTGATGCAGCACATGAGACTTACGAAAATAATTT
  TGATCATCTTAATTTTGATTTGGATTTGATGCCTTGGGAGTCAGACATATGGAGCCCCAGCAGCCACTTC
  TGCTCAGACATTAAGGCAGAGCCCCAGCCTCTTTCTCCGGCTTCCTCCAGTTGTTCTGTCTCTTCTCCTC
  GGTCCACAGACTCCTGTTCTTCAACTCAGCACGTTCCTGAGGAGTTGGACTTGTTGTCTAGTTCTCAGTC
  TCCCCTTTCCTTGTATGGCGACAGCTGTCACAGCCCCTCCTCAGCAGAGCCACTGAAGGAAGAGAAGCCT
  GTCACTGGTCCTGGAAACAAAACGGAACATGGACTGACTCCAAAGAAAAAAATCCAGATGAGTTCAAAAC
  CTTCAGTTCAGCCCAAGCCTTTGTTACTTCCAGCAGCACCCAAGACTCCAGCAAACGCCAGTGTCCCAGC
  AAAGACCATCATCATTCAGACACTGCCAGCCCTGATGCCACTGGCAAAGCAGCAGTCGATTATCAGTATA
  CAGCCTGCGCCCACCAAAGGTCAGACTGTTTTGCTCTCTCAGCCTGCTGTGGTTCAACTTCAGACCCCTG
  GAGTTCTGCCCTCTGCTCAGCCAGTACTTGCTGTCACTGGCGGAGCCACACAGCTACCTAACCATGTGGT
  GAATGTGGTGCCAGCCCCGGTGGTGAACAGCCCGGTGAACGGAAAACTGTGTGTGACTAAACCTGTTCTA
  CAAAGCAGCACCAGAAGCACGGGTTCAGATATTGCCGTACTGAGGAGACAGCAGCGTATGATCAAGAACC
  GAGAGTCTGCTTGTCAGTCACGAAAGAAGAAGAAAGAGTATATGCTAGGCCTGGAGGCCAGGTTGAAGGC
  CGCCCTCTCCGAGAATGAGCAGCTGAAGAAGGAGAATGGGTCCCTGAAGCGACAGCTGGACCAGGTGGTG
  TCAGAGAACCAGAGGCTCAAAGTCCCAAGTCCAAAGCGCAGAGCTGTCTGTGTGATGATAGTATTAGCAT
  TTATCATGCTGAACTATGGGCCCATGAGCATGCTGGAGCAAGATTCCAGGAGAGTGAAACCTAGTGTGAG
  CCCTGCCAATCAAAGGAGGCATCTTTTAGAGTTTTCAGCAAAAGAAGTTAAAGACACGTCAGATGGTGAC
  AACCAGAAAAACAGTTACAGATATGATCATTCTGTGTCCAATGACAAAGCCTTAATGGTGCTAAGTGAAG
  AACCATTACTTTATATCCCTCCACCTCCATGTCAGCCCCTCATTAACACGACAGAGTCTCTCAGGTTGAA
  CCATGAACTTCGAGGCTGGGTTCATAGACATGAAGTGGAAAGGACCAAATCGAGAAGAATGACAAATAGC
  CAGCAGAAAACCCGCATTCTCCAGGGTGCTCTGGAACAGGGCTCGAATTCTCAGCTGATGGCTGTCCAGT
  ACACAGAAACCACTAGCATCAGTAGGAATTCTGGGAGTGAGCTGCAGGTGTATTACGCTTCGCCTGGAAG
  TTACCAAGGCTTCTTTGACGCCATCCGCAGGAGGGGAGATACATTTTACGTCGTATCATTTCGAAGGGAT
  CACCTGCTATTACCAGCTACCACCCATAACAAGACCACAAGACCGAAGATGTCCATTGTGTTACCAGCAA
  TAAACATAAATGATAACGTGATCAATGGGCAGGATTATGAAGTAATGATGCAGATTGACTGTCAGGTCAT
  GGACACCAGGATCCTCCACATCAAAAGCTCCTCGGTTCCCCCGTATCTCCGAGATCATCAGAGAAACCAA
  ACCAGCACCTTCTTCGGTTCCCCTCCCACAGCCACAGAGACGACCCATGTGGTCAGCACCCTCCCTGAGT
  CGGTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204267 representing NM_001107196
  Red=Cloning site Green=Tags(s)
  
  MESPFSPVLPHGPGEDWESTLFAELGYFTDTDDVQFDAAHETYENNFDHLNFDLDLMPWESDIWSPSSHF
  CSDIKAEPQPLSPASSSCSVSSPRSTDSCSSTQHVPEELDLLSSSQSPLSLYGDSCHSPSSAEPLKEEKP
  VTGPGNKTEHGLTPKKKIQMSSKPSVQPKPLLLPAAPKTPANASVPAKTIIIQTLPALMPLAKQQSIISI
  QPAPTKGQTVLLSQPAVVQLQTPGVLPSAQPVLAVTGGATQLPNHVVNVVPAPVVNSPVNGKLCVTKPVL
  QSSTRSTGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKAALSENEQLKKENGSLKRQLDQVV
  SENQRLKVPSPKRRAVCVMIVLAFIMLNYGPMSMLEQDSRRVKPSVSPANQRRHLLEFSAKEVKDTSDGD
  NQKNSYRYDHSVSNDKALMVLSEEPLLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNS
  QQKTRILQGALEQGSNSQLMAVQYTETTSISRNSGSELQVYYASPGSYQGFFDAIRRRGDTFYVVSFRRD
  HLLLPATTHNKTTRPKMSIVLPAININDNVINGQDYEVMMQIDCQVMDTRILHIKSSSVPPYLRDHQRNQ
  TSTFFGSPPTATETTHVVSTLPESVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107196
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107196.1, NP_001100666.1
• RefSeq Size: 3217 bp
• RefSeq ORF: 1971 bp
• Locus ID: 304962
• UniProt ID: G3V909
• Cytogenetics: 13q24
• MW: 72.7 kDa
• Gene Summary: Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina (By similarity).[UniProtKB/Swiss-Prot Function]