Cdh20 (NM_001012748) Rat Tagged ORF Clone

CAT#: RR204105

Cdh20 (Myc-DDK-tagged ORF) - Rat cadherin 20 (Cdh20), (10 ug)

Product Images

Circular map for RR204105

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdh20
• Synonyms: Cad20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204105 representing NM_001012748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACTACAGGTAGAATGAGCAATGCAAAGAGCTGGCTTGGACTTGGCACATCCTTGTACTTCTGGG
  CACTGATGGACCTTGCGACCACCGTTCTGTCCAGCACTCCGATGCCGGAAGTCGAGTTAGATACACTCTT
  CTCAGGAAAGCCACAGTCACATCAGCGGAGCAGGAGGAGCTGGGTTTGGAACCAATTTTTTGTCCTGGAA
  GAGTACACTGGGACTGACCCTCTGTATGTCGGCAAGCTTCATTCAGACATGGACAGGGGAGATGGATCCA
  TCAAATACATCCTTTCAGGAGAAGGAGCAGGGATCGTGTTTACCATTGATGACACCACTGGAGACATCCA
  TGCCATTCAGAGGCTTGATCGAGAGGAAAGAGCCCAATATACTCTAAGAGCTCAAGCCCTAGACAGGAGA
  ACAGGCCGGCCAATGGAGCCAGAATCAGAGTTTATTATCAAAATTCAAGATATCAATGACAACGAGCCCA
  AGTTCCTGGATGGACCTTACGTTGCCACTGTGCCAGAAATGTCACCAGTAGGTACCTCTGTTATCCAAGT
  GACAGCCACAGATGCTGATGACCCAACCTATGGCAACAGTGCCCGAGTTGTGTACAGCATTCTCCAGGGC
  CAGCCATATTTTTCTGTGGACTCCAAAACAGGTATAATCAGAACAGCGCTCATGAACATGGACAGAGAAG
  CCAAGGAATACTATGAAGTGATTATCCAAGCCAAGGATATGGGGGGTCAACTTGGAGGATTGGCTGGGAC
  CACAACAGTCAACATCACACTCTCAGATGTCAATGATAACCCACCCCGGTTTCCACAGAAACATTATCAG
  ATGAGTGTGCTGGAGTCCGCTCCTGTTAGCTCGACTGTGGGGAGAGTGTTTGCCAAAGACCTGGACGAAG
  GCATCAATGCAGAAATGAAGTACACCATTGTGGATGGAGATGGTGCAGACGCTTTTGATATCAATACAGA
  CCCGAACTTCCAAGTTGGCATCATAACTGTGAAGAAGCCTTTGAGTTTTGAAAGCAAGAAAAGCTACACC
  CTGAAGGTGGAAGGATCCAATCCTCACGTAGAGATGCGTTTTCTGAACCTTGGCCCATTTCAGGACACAA
  CAACAGTGCACATCATTGTGGAGGATGTGGACGAGCCCCCTGTGTTTGAGCCCCGCTTTTATTTTGTGGA
  GGTGCCAGAGGATGTGACAATTGGAACAACCATACAGATCATTTCTGCCAAGGACCCAGACATGACCAAC
  AACTCAATCAGATACTCCATTGACAGAGGCAGCGACCCTGGAAGATTTTTCTATGTTGACATTACAACAG
  GTGCTCTAATGACTGCAAGACCACTAGACAGAGAAGAGTTTTCTTGGCATAATATAACTGTTCTTGCTAT
  GGAAATGAACAACCCCTCCCAAGTGGGAAGTGTTGCTGCCACAATCAAAGTCCTAGATGTGAATGACAAT
  GCTCCAGAGTTTCCCAGATTCTATGAAGCTTTCATCTGTGAAAATGCCAAAGCGGGACAGCTGATCCAGA
  CAGTGAGTGCTGTGGATCAAGATGACCCTCACAATGGTCAACATTTCTACTACAGTTTGGCTCCTGAGGC
  TGCTAACAACCCCAACTTCACTGTCAGGGACAACCAAGATAATACCGCTCGCATTCTGACCAGGAGGTCC
  GGCTTCCGACAGCAGGAGCAGAGCGTCTTCTACCTGCCCATCCTCATAGCAGACAGCGGCCAGCCGGTCC
  TCAGCAGCACCGGCACACTTACCATTCAAGTGTGCAGCTGCAGTGACGATGGCCACGTCATGTCCTGCAG
  CCCAGAGGCATATATGCTCCCAGTCAGTTTGAGTCGGGGTGCCCTCATCGCCATCCTGGCCTGCGTCTTT
  GTTCTCTTAGTATTGGTGCTGCTCATCCTGTCCATGAGGCGCCACCGAAAACAACCCTACATCATCGACG
  ACGACGAGAACATCCACGAGAACATTGTGCGCTATGACGACGAGGGGGGAGGCGAAGAGGACACAGAGGC
  CTTCGACATCGCCGCCATGTGGAACCCGCGCGAGGCGCAGGCGGGCGCCGCCCCGAAGACGCGGCAGGAC
  ATGCTCCCCGAGATCGAGAGCCTCTCCCGCTACGTGCCTCAGACGTGCGCGGTCAGCAGCACGGTCCACA
  GCTACGTGCTGGCCAAGCTCTACGAGGCCGACATGGACCTGTGGGCCCCGCCCTTCGATTCCCTGCAGAC
  CTACATGTTTGAGGGGGACGGCTCCGTGGCCGGCTCGCTCAGCTCGCTTCAGTCAGCCACCTCGGACTCA
  GAGCAGAGCTTTGATTTCCTCACGGACTGGGGACCTCGCTTCCGGAAACTGGCAGAACTCTACGGGGCGT
  CGGAGGGGCCCTCGCCCTTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204105 representing NM_001012748
  Red=Cloning site Green=Tags(s)
  
  MWTTGRMSNAKSWLGLGTSLYFWALMDLATTVLSSTPMPEVELDTLFSGKPQSHQRSRRSWVWNQFFVLE
  EYTGTDPLYVGKLHSDMDRGDGSIKYILSGEGAGIVFTIDDTTGDIHAIQRLDREERAQYTLRAQALDRR
  TGRPMEPESEFIIKIQDINDNEPKFLDGPYVATVPEMSPVGTSVIQVTATDADDPTYGNSARVVYSILQG
  QPYFSVDSKTGIIRTALMNMDREAKEYYEVIIQAKDMGGQLGGLAGTTTVNITLSDVNDNPPRFPQKHYQ
  MSVLESAPVSSTVGRVFAKDLDEGINAEMKYTIVDGDGADAFDINTDPNFQVGIITVKKPLSFESKKSYT
  LKVEGSNPHVEMRFLNLGPFQDTTTVHIIVEDVDEPPVFEPRFYFVEVPEDVTIGTTIQIISAKDPDMTN
  NSIRYSIDRGSDPGRFFYVDITTGALMTARPLDREEFSWHNITVLAMEMNNPSQVGSVAATIKVLDVNDN
  APEFPRFYEAFICENAKAGQLIQTVSAVDQDDPHNGQHFYYSLAPEAANNPNFTVRDNQDNTARILTRRS
  GFRQQEQSVFYLPILIADSGQPVLSSTGTLTIQVCSCSDDGHVMSCSPEAYMLPVSLSRGALIAILACVF
  VLLVLVLLILSMRRHRKQPYIIDDDENIHENIVRYDDEGGGEEDTEAFDIAAMWNPREAQAGAAPKTRQD
  MLPEIESLSRYVPQTCAVSSTVHSYVLAKLYEADMDLWAPPFDSLQTYMFEGDGSVAGSLSSLQSATSDS
  EQSFDFLTDWGPRFRKLAELYGASEGPSPLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012748
• ORF Size: 2403 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012748.1, NP_001012766.1
• RefSeq Size: 2450 bp
• RefSeq ORF: 2406 bp
• Locus ID: 363948
• UniProt ID: Q5DWV1
• Cytogenetics: 13p11
• MW: 89.1 kDa
• Gene Summary: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity).[UniProtKB/Swiss-Prot Function]