Vmp1 (NM_138839) Rat Tagged ORF Clone

CAT#: RR203664

Vmp1 (Myc-DDK-tagged ORF) - Rat transmembrane protein 49 (Tmem49), (10 ug)

Product Images

Circular map for RR203664

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vmp1
• Synonyms: Tmem49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203664 representing NM_138839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGAATGGAACAAATTGTGACCAGAGACGTGGAGCAATGAGTAAGGAACAGCATAATGGGAGTT
  TCACAGACCCCTCTTCAGTAAATGAGAAGAAGAGAAGGGATCGGGAAGAAAGACAGAATATCGTCCTGTG
  GAGACAGCCACTCATTACCTTGCAGTATTTCTCTCTGGAAACTCTTGTAGTTTTGAAGGAATGGACCTCA
  AAATTGTGGCATCGTCAAAGCATGGTGGTGTCCTTTTTCCTGCTGCTTGCTGCGCTTGTAGCTACGTATT
  ATGTGGAAGGAGCGCACCAGCAGTATGTGCAGCGGATAGAGAAGCAGTTTCTTTTATATGCCTACTGGAT
  AGGCCTGGGGATTCTGTCCTCTGTTGGTCTTGGAACAGGATTGCACACCTTTCTGCTTTATCTGGGCCCA
  CATATAGCTTCAGTTACATTAGCTGCTTATGAATGCAATTCGGTTAATTTCCCTGAGCCACCCTATCCTG
  ACCAGATTATCTGCCCAGACGAAGAAGGCACTGAAGGAGCCATATCTTTGTGGAGTATCATCTCAAAAGT
  TAGAATTGAAGCCTGCATGTGGGGCATTGGGACAGCAATTGGGGAGCTGCCTCCATATTTCATGGCCAGG
  GCAGCTCGCCTCTCAGGTGCTGAACCAGATGATGAAGAGTATCAGGAATTTGAAGAAATGCTGGAACACG
  CAGAGACTGCACAAGACTTTGCATCACGAGCTAAACTGGCAGTTCAAAAACTAGTACAGAAAGTTGGATT
  TTTTGGAATTTTGGCCTGTGCTTCTATTCCAAATCCCCTGTTCGACCTGGCTGGAATAACATGTGGACAC
  TTCCTTGTACCTTTTTGGACCTTCTTTGGTGCAACCCTGATTGGAAAAGCAATCATTAAAATGCATATCC
  AGAAAATCTTTGTTATAGTAACTTTCAGCAAACACATCGTGGAGCAGATGGTGGCTTTCATTGGGGCTGT
  ACCCGGCATAGGTCCGTCTCTGCAGAAGCCTTTTCAGGAATACCTGGAGGCCCAGCGGCAGAAGCTCCAC
  CATAGAAGCGAAGCAGGCACAGCGCAGGGAGAAAACTGGTTATCCTGGACGTTTGAGAAGCTGGTCGTTG
  CCATGGTGTGTTACTTTATCCTGTCTATCATTAACTCCATGGCACAAAGCTATGCCAAACGAATCCAGCA
  GCGCTTGAACTCAGAGGAGAAGACTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203664 representing NM_138839
  Red=Cloning site Green=Tags(s)
  
  MAENGTNCDQRRGAMSKEQHNGSFTDPSSVNEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTS
  KLWHRQSMVVSFFLLLAALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGP
  HIASVTLAAYECNSVNFPEPPYPDQIICPDEEGTEGAISLWSIISKVRIEACMWGIGTAIGELPPYFMAR
  AARLSGAEPDDEEYQEFEEMLEHAETAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGH
  FLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLH
  HRSEAGTAQGENWLSWTFEKLVVAMVCYFILSIINSMAQSYAKRIQQRLNSEEKTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138839
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138839.2, NP_620194.1
• RefSeq Size: 1993 bp
• RefSeq ORF: 1221 bp
• Locus ID: 192129
• UniProt ID: Q91ZQ0
• Cytogenetics: 10q26
• MW: 45.9 kDa
• Gene Summary: stress-induced gene; may promote formation of intracellular vacuoles followed by cell death [RGD, Feb 2006]