Chml (NM_001109524) Rat Tagged ORF Clone

CAT#: RR203636

Chml (Myc-DDK-tagged ORF) - Rat choroideremia-like (Rab escort protein 2) (Chml), (10 ug)

Product Images

Circular map for RR203636

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chml
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203636 representing NM_001109524
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACAAACTTCCCACAGAGTTTGATGTGGTTATAATAGGGACTGGTTTGCCTGAGTCCATCCTGG
  CAGCTGCATGTTCAAGAAGTGGACAGAGAGTTCTGCACGTTGACTCAAGGAGTTACTATGGAGGGAATTG
  GGCGAGTTTCAGCTTTACAGGTTTGCTGTCGTGGCTGAAGGACTATCAACAGAACCATGACAGCGAGGGA
  GACGTGACTGGCACATGGCAAGACCTGATCGATGAAACCGAAGAAGCCATCGCTCTTTGCAAGAAGGACG
  AAACTATTCAGCACACGGAAGTCTTCTGTTATGCTAGTCAAGATGTGGAAGATAGCATGCAAGAGACCGA
  CCCTCTGCAGAAAAGTCCCTCCCCAGAGGCATCTGCTACTCCGGCGGACTCTCTGGATACTGCAAGCTTG
  CCCAAAGAAAGGCACTCAGAAGACTGTACAAGCTACGAGGCGCCTTCTAGACGTACAGAGGGAAGTGATG
  GAGCAGTTTCACTCTCCTCCGCTGATGCACAGGACTCACTGGAGAAAGAAAAGTTCTGTGGAGATGAAAC
  CGGGATGCACACAGTTTCAGATGGAGAAGAAGGTGAACACAAACTTGCTGCAGAAGACAACGTAGGACAA
  CCAAAGAGAAATACGATTACTTACTCGCAGATGGTCAAGGAGAGCCGGACATTCAACATTGACTTGGTAT
  CAAAGCTGCTGTATTCTCAAGGCTCACTGATCGATCTTCTAATCAAATCAAACGTTAGTCGTTATGCAGA
  GTTTAAGAATGTTACTAGGATCCTTGCATTTCGGGAAGGGAAGGTAGAACAGGTGCCTTGCTCCAGAGCA
  GATGTGTTTAACAGTAAAGAGCTCACTATGGTTGAGAAGAGAATGCTAATGAAGTTTCTCACGTTCTGTT
  TAGACTACGAGCAACATTCAGATGAATACCAAGATTTCAAACAATGTTCATTTTCTGACTACTTAAAAAC
  TAAAAAGCTAACGCCTAACCTCCAACACTTCATACTCCACTCAATTGCAATGTCGTCAGACTCATCCTGC
  ACTACATTAGATGGCCTTCAAGCAACAAAGAACTTCCTTCGGTGTCTTGGACGGTTTGGCAACACTCCCT
  TTATATTCCCTTTATACGGCCAAGGAGAAATTCCCCAGTGTTTCTGCAGGATGTGCGCAGTTTTTGGTGG
  AGTCTATTGCCTTCGCCATAAGGTACAATGTCTTGTAGTTGACAGAGACTCTGGAAGATGTAAAGGAATC
  ATAGATGGCTTTGGGCAGAGAATAAGTGCCAACTATTTTATCGTGGAGGACAGTTACCTTTCTAAGGAAA
  TGTGTTCAAACATTCAGTATAAGCAGATCTCAAGGGCAGTGCTTATCACAGATCAGTCCATCCTGAAGAC
  TGATTCAGACCAGCAGATTTCCATTTTGGTAGTGCCTCCTCTGGAGCCGGGAACTGCTTCGGTTCGGGTC
  ATGGAATTATGTTCCTCAACCATGACATGCATGAAGGACAGCTATCTGGTCCACTTGACCTGTTTCTCTT
  CAAAAACAGCCCGAGAAGACTTGGAACCCGTGGTGAAGCAGTTATTCATCCCTTTTGCAGAAGCAGAAGC
  AGACAAAGAGGAACTCAGAAAACCAAGACTTTTGTGGGCCCTTTATTTTAATATGAGAGATTCCTCAGGG
  GTGAGCCGAAGCTCATACTGTAGCTTACCTTCCAATGTATACATCTGCTCTGGTCCTGACTGTGGACTGG
  GGAATGAGCATGCAGTCAAGCAAGCGGAAACACTGTTCCAGGAGATCTTTCCCAGTGAAGAGTTCTGCCC
  TCCTCCACCAAACCCTGAAGACATTATCTTTGAAGCGGAGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203636 representing NM_001109524
  Red=Cloning site Green=Tags(s)
  
  MADKLPTEFDVVIIGTGLPESILAAACSRSGQRVLHVDSRSYYGGNWASFSFTGLLSWLKDYQQNHDSEG
  DVTGTWQDLIDETEEAIALCKKDETIQHTEVFCYASQDVEDSMQETDPLQKSPSPEASATPADSLDTASL
  PKERHSEDCTSYEAPSRRTEGSDGAVSLSSADAQDSLEKEKFCGDETGMHTVSDGEEGEHKLAAEDNVGQ
  PKRNTITYSQMVKESRTFNIDLVSKLLYSQGSLIDLLIKSNVSRYAEFKNVTRILAFREGKVEQVPCSRA
  DVFNSKELTMVEKRMLMKFLTFCLDYEQHSDEYQDFKQCSFSDYLKTKKLTPNLQHFILHSIAMSSDSSC
  TTLDGLQATKNFLRCLGRFGNTPFIFPLYGQGEIPQCFCRMCAVFGGVYCLRHKVQCLVVDRDSGRCKGI
  IDGFGQRISANYFIVEDSYLSKEMCSNIQYKQISRAVLITDQSILKTDSDQQISILVVPPLEPGTASVRV
  MELCSSTMTCMKDSYLVHLTCFSSKTAREDLEPVVKQLFIPFAEAEADKEELRKPRLLWALYFNMRDSSG
  VSRSSYCSLPSNVYICSGPDCGLGNEHAVKQAETLFQEIFPSEEFCPPPPNPEDIIFEAEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109524
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109524.1, NP_001102994.1
• RefSeq Size: 1866 bp
• RefSeq ORF: 1866 bp
• Locus ID: 689102
• Cytogenetics: 13q25
• MW: 69.4 kDa