Col5a1 (NM_134452) Rat Tagged ORF Clone

CAT#: RR203050

Col5a1 (Myc-DDK-tagged ORF) - Rat collagen, type V, alpha 1 (Col5a1), (10 ug)

Product Images

Circular map for RR203050

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col5a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203050 representing NM_134452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTACACACCCGCTGGAAAGCTCCGCGCCCGGGCGCCCCGCTGCTGTCCTCGCCGCTGCTCCTGC
  TCCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAACAGATCTTCTGGAGATGCTAGA
  TTTTCACAATTTGCCCTCAGGCGTAACGAAAACCACAGGTTTCTGTGCCACTCGACGATCTTCCAAAGAG
  CCGGATGTTGCCTACCGAGTCTCTAAAGATGCACAGCTCAGCATGCCCACCAAGCAGCTGTACCCTGAGT
  CTGACTTTCCTGAGGACTTCTCCATCCTGACAACCGTGAAAGCCAAGAAAGGCAGTCAGGCCTTCCTGGT
  CTCCGTTTACAATGAGCAGGGCATCCAGCAGTTGGGACTGGAGCTGGGCCGCTCCCCTGTCTTCCTCTAT
  GAGGACCACACAGGGAAGCCCGGGCCTGAAGAGTATCCACTCTTCCCTGGCATCAACCTGTCCGATGGCA
  AGTGGCACCGAATCGCTATCAGTGTATACAAGAAAAATGTCACCTTGATCCTCGACTGTAAAAAGAAGAT
  CACGAAGTTCCTCAACCGCGGCGACCACCCCATAATAGATGTCAACGGTATCATCATGTTTGGCTCCCGG
  ATTCTGGATGATGAAATATTTGAGGGTGACATCCAACAGTTGCTCTTCGTCTCCGACCACCGAGCTGCCT
  ATGATTACTGTGAGCACTACAGCCCCGACTGTGACACAGCGGTCCCTGACACACCTCAGTCACAGGACCC
  TAACCCAGATGAATATTACCCAGAAGGAGAGGGTGAGACCTATTACTATGAGTACCCATATTATGAAGAC
  CCTGAAGACCCGGGAAAGGAGCCCGCCCCCTCTCAGAAGCCAGTGGAAGCCGCCAGAGAAACCACGGAGG
  TTCCTGAGGAGCAGACCCAGCCCCCATCCGAAGCCCCTACAGTGCCTGAGACCAGTGACACGGCTGGCAA
  GGAGGACAATCCAGGGATCGGGGACTACGACTATGTGCCCACTGACGACTACTACACTACATCCCCGTAT
  GAAGACTTCGGCTATGGCGAGGGCGTGGAGAACCCCGACCAGCCCACCAACCCTGACTCAGGGGCCGAGA
  TCCCCACCAGCACCAGTGTTACCTCCAATTCCTCCAATCCGGCTCCATCTCCAGAGGAAGGGAAGGATGA
  CCTGGGAGGTGAGTTCACCGAGGAGACCATCAAGAATCTAGATGAAAACTACTACGACCCGTACTTTGAC
  CCGGACTCCGACTCCAATGTCTCTCCATCGGAGATAGGGCCAGGCATGCCTGCTAACCAGGACACCATCT
  ATGAAGGGATTGGAGGACCCCGAGGTGAGAAAGGGCAAAAGGGAGAACCAGCCATCATTGAGCCGGGAAT
  GCTGATAGAGGGGCCCCCTGGCCCTGAAGGCCCTGCTGGTCTTCCAGGACCTCCAGGAACTACGGGTCCT
  ACTGGCCAAATGGGTGACCCTGGAGAAAGGGGTCCCCCTGGGCGCCCAGGTCTTCCTGGGGCTGATGGCT
  TGCCAGGTCCCCCCGGGACCATGCTCATGCTGCCGTTCCGTTTTGGAGGTGGTGGCGACGCCGGTTCTAA
  GGGCCCCATGGTCTCTGCCCAGGAATCCCAGGCACAGGCTATCCTTCAGCAAGCCAGGTTGGCACTAAGG
  GGACCAGCTGGTCCAATGGGTCTCACCGGGAGACCTGGCCCCATGGGTCCTCCTGGGAGTGGAGGTTTGA
  AGGGTGAGCCAGGAGACATGGGACCTCAGGGTCCTCGAGGTGTGCAAGGTCCACCCGGCCCAACAGGGAA
  GCCTGGAAGACGGGGCCGTGCTGGGAGTGATGGTGCCAGAGGCATGCCTGGACAAACAGGCCCCAAGGGT
  GACCGTGGCTTTGATGGTCTGGCTGGGTTGCCGGGAGAGAAAGGCCATAGAGGTGACCCTGGTCCTTCTG
  GCCCGCCGGGACTCCCAGGAGATGATGGAGAAAGGGGTGACGATGGAGAAGTTGGGCCCAGGGGGCTGCC
  TGGGGAGCCTGGACCACGTGGTCTGCTTGGGCCAAAAGGCCCCCCTGGCCCACCTGGACCTCCTGGTGTG
  ACGGGTATGGATGGCCAGCCTGGCCCCAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGGCCCCCAGGAC
  AGCAGGGTAATCCTGGTGCCCAGGGTCTTCCCGGTCCCCAGGGTGCCATTGGTCCTCCAGGAGAAAAGGG
  TCCTTTGGGGAAACCAGGTCTCCCAGGAATGCCCGGAGCTGATGGACCCCCGGGGCACCCTGGCAAAGAA
  GGTCCTCCAGGAGAGAAAGGAGGCCAGGGTCCTCCCGGCCCCCAGGGTCCCATTGGCTACCCAGGTCCAC
  GAGGAGTCAAGGGGGCAGATGGCATCCGAGGGCTGAAGGGCACCAAGGGAGAGAAGGGTGAAGACGGCTT
  CCCTGGGTTTAAAGGCGACATGGGAATAAAGGGTGACCGGGGGGAGATCGGCCCACCCGGCCCCCGAGGA
  GAAGATGGTCCTGAAGGCCCAAAAGGTCGAGGTGGTCCCAATGGTGATCCTGGTCCCCTGGGGCCCACCG
  GGGAGAAGGGGAAGCTCGGAGTGCCAGGATTACCAGGGTACCCAGGAAGACAAGGGCCAAAGGGTTCTAT
  TGGATTCCCTGGCTTCCCGGGCGCCAACGGAGAGAAAGGTGGCAGGGGGACACCTGGAAAGCCAGGACCA
  CGGGGACAGCGAGGCCCAACGGGCCCGAGGGGTGAACGAGGCCCACGGGGCATCACTGGGAAGCCTGGCC
  CTAAGGGCAACTCAGGAGGTGATGGCCCAGCCGGCCCTCCTGGTGAGCGGGGACCCAATGGACCCCAAGG
  CCCCACCGGCTTCCCTGGACCTAAGGGTCCCCCGGGTCCACCAGGCAAGGACGGGCTCCCCGGACATCCT
  GGGCAGAGAGGGGAGACTGGTTTCCAAGGCAAGACTGGCCCTCCAGGCCCTCCAGGAGTGGTTGGCCCTC
  AGGGTCCCACAGGCGAGACGGGTCCCATGGGGGAGCGTGGCCACCCTGGTCCTCCAGGCCCCCCTGGTGA
  ACAGGGCCTCCCAGGTGCTGCTGGGAAAGAAGGGACGAAGGGTGACCCAGGTCCTGCTGGCCTCCCTGGG
  AAGGATGGACCTCCAGGATTGCGTGGATTCCCTGGGGACCGAGGGCTCCCCGGCCCCGTGGGAGCACTCG
  GACTGAAAGGCAGCGAAGGCCCCCCTGGCCCCCCAGGTCCTGCGGGTTCTCCAGGGGAGAGAGGACCAGC
  TGGTGCTGCTGGACCCATTGGAATTCCAGGGAGACCTGGGCCTCAGGGACCTCCGGGGCCTGCTGGAGAG
  AAAGGGGTTCCTGGAGAGGAAGGTCCGCAAGGCCCGGCTGGCCGAGATGGCCTGCAAGGTCCCGTGGGGC
  TCCCTGGACCAGCCGGCCCCGTGGGTCCTCCTGGAGAAGATGGAGATAAGGGAGAGATCGGAGAGCCCGG
  GCAGAAAGGGAGCAAGGGCGACAAAGGCGAGCAGGGTCCTCCTGGGCCTACCGGTCCTCAAGGCCCCACT
  GGGCAGCCAGGCCCTTCGGGAGCAGATGGTGAACCTGGCCCTCGTGGACAGCAGGGCCTGTTTGGGCAGA
  AAGGAGATGAAGGTTCAAGAGGTTTCCCAGGACCGCCAGGGCCAGTGGGCTTGCAGGGTTTACCAGGACC
  TCCAGGAGAAAAGGGTGAGACAGGAGACGTGGGCCAGATGGGCCCTCCTGGTCCACCAGGCCCCCGAGGA
  CCCTCTGGAGCTCCAGGAGCTGATGGGCCACAGGGTCCTCCAGGAGGGATTGGCAACCCTGGTGCAGTCG
  GAGAAAAGGGAGAGCCTGGCGAAGCTGGAGAGCCTGGCCTTCCAGGAGAAGGAGGTCCCCTGGGACCTAA
  AGGAGAAAGAGGGGAGAAGGGAGAGGCTGGCCCCTCTGGTGCTGCTGGACCCCCTGGACCCAAAGGCCCA
  CCTGGAGATGATGGCCCCAAAGGCAGCCCCGGCCCCGTGGGCTTTCCTGGAGACCCTGGGCCCCCTGGAG
  AGCCAGGCCCAGCAGGTCAAGATGGCCCACCTGGTGACAAAGGGGATGATGGAGAACCTGGGCAGACGGG
  ATCCCCAGGCCCTACTGGTGAACCTGGTCCATCTGGGCCTCCAGGAAAGAGGGGTCCACCGGGCCCTGCA
  GGCCCTGAAGGCAGGCAGGGGGAGAAAGGAGCCAAGGGAGAAGCTGGCTTAGAAGGCCCTCCTGGGAAGA
  CTGGCCCCATTGGTCCCCAAGGGGCCCCTGGGAAGCCTGGCCCTGATGGTCTTCGTGGGATCCCTGGTCC
  TGTGGGTGAGCAAGGCCTCCCAGGATCCCCAGGCCCTGATGGTCCACCCGGCCCCATGGGTCCTCCAGGA
  CTCCCTGGCCTCAAAGGAGACTCCGGTCCCAAAGGTGAAAAGGGCCATCCGGGCCTGATTGGACTCATCG
  GACCTCCGGGCGAACAAGGTGAAAAAGGTGACCGAGGACTCCCAGGCCCCCAGGGCTCATCTGGTCCTAA
  AGGAGAACAGGGCATTACAGGTCCTTCTGGCCCGCTTGGGCCTCCTGGTCCCCCAGGCTTGCCGGGCCCT
  CCAGGTCCCAAAGGTGCTAAAGGTTCATCGGGTCCCACTGGCCCGAAGGGCGAGGCAGGCCATCCAGGAC
  TCCCTGGTCCACCCGGGCCTCCAGGCGAGGTCATCCAGCCCCTGCCAATCCAGGCGTCCAGGACTCGGCG
  GAACATTGATGCCAGTCAGCTCCTGGATGATGGGGCTGGGGAGAGCTACGTGGATTATGCAGATGGCATG
  GAAGAGATCTTTGGTTCCCTCAACTCCCTGAAGCTGGAGATTGAGCAGATGAAGCGGCCACTGGGCACAC
  AGCAGAACCCAGCCCGTACCTGCAAGGACCTTCAACTCTGCCATCCTGATTTCCCAGACGGCGAATACTG
  GGTCGATCCCAACCAAGGGTGCTCCAGGGACTCCTTCAAAGTCTACTGCAATTTCACAGCTGGAGGGTCC
  ACGTGCGTCTTCCCTGACAAGAAGTCTGAGGGAGCCAGAATCACTTCTTGGCCCAAAGAAAACCCAGGTT
  CCTGGTTCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGATGCTGAAGGCAACCCTGTGGGCGT
  GGTACAAATGACCTTCCTGCGGCTGCTGAGTGCCTCTGCCCAGCAGAACATCACCTACAACTGCTACCAG
  TCCGTGGCCTGGCAGGATGCCGCCACAGGCAGCTACGATAAGGCTATCCGCTTCTTGGGCTCCAACGATG
  AGGAAATGTCTTATGATAACAACCCCTACATCCGTGCCCTGGTGGATGGCTGTGCTACCAAGAAAGGCTA
  CCAGAAGACGGTGCTGGAGATTGACACGCCCAAAGTTGAGCAAGTCCCCATTGTGGACATCATGTTCACC
  GACTTTGGTGAAGCCTCACAGAAATTTGGATTTGAAGTAGGGCCAGCTTGCTTCCTAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203050 representing NM_134452
  Red=Cloning site Green=Tags(s)
  
  MDVHTRWKAPRPGAPLLSSPLLLLLLLLWAPPPSRAAQPTDLLEMLDFHNLPSGVTKTTGFCATRRSSKE
  PDVAYRVSKDAQLSMPTKQLYPESDFPEDFSILTTVKAKKGSQAFLVSVYNEQGIQQLGLELGRSPVFLY
  EDHTGKPGPEEYPLFPGINLSDGKWHRIAISVYKKNVTLILDCKKKITKFLNRGDHPIIDVNGIIMFGSR
  ILDDEIFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYED
  PEDPGKEPAPSQKPVEAARETTEVPEEQTQPPSEAPTVPETSDTAGKEDNPGIGDYDYVPTDDYYTTSPY
  EDFGYGEGVENPDQPTNPDSGAEIPTSTSVTSNSSNPAPSPEEGKDDLGGEFTEETIKNLDENYYDPYFD
  PDSDSNVSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGP
  TGQMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALR
  GPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKG
  DRGFDGLAGLPGEKGHRGDPGPSGPPGLPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGV
  TGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKE
  GPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRG
  EDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGP
  RGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHP
  GQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPG
  KDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGE
  KGVPGEEGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPT
  GQPGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRG
  PSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGP
  PGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPA
  GPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPG
  LPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPLGPPGPPGLPGP
  PGPKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGM
  EEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGS
  TCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAQQNITYNCYQ
  SVAWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFT
  DFGEASQKFGFEVGPACFLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134452
• ORF Size: 5520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134452.1, NP_604447.1
• RefSeq Size: 5551 bp
• RefSeq ORF: 5523 bp
• Locus ID: 85490
• UniProt ID: Q9JI03
• Cytogenetics: 3p12
• MW: 184 kDa
• Gene Summary: alpha chain of type V collagen; binds heparin with high affinity [RGD, Feb 2006]